Changes in culture medium pH by cell suspension cultures of Dioscorea deltoidea

Butenko, R. G.; Lipsky, Alexander; Chernyak, N. D.; Arya, H. C.

doi:10.1016/0304-4211(84)90230-x

Cited by 20 publications

(9 citation statements)

References 10 publications

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“…In cell suspension cultures of Dioscorea deltoidea , there was an acidification of the culture medium during the lag phase, an alkalization during the exponential phase, and a steady state of pH in the stationary phase. At the same time, the exponential growth of the cell culture medium may be associated with the development of alkaline reactions that occur during metabolism [ 36 ]. The observed changes in pH values may be related to the use of ammonium and nitrate or the absorption of sugar by the transport mechanism with H + [ 37 ].…”

Section: Resultsmentioning

confidence: 99%

Establishment of a Cell Suspension Culture of Ageratina pichinchensis (Kunth) for the Improved Production of Anti-Inflammatory Compounds

Sánchez-Ramos

Álvarez

Romero-Estrada

et al. 2020

Plants

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Ageratina pichinchensis (Kunth) is a plant used in traditional Mexican medicine to treat multiple ailments. However, there have not been biotechnological studies on producing compounds in in vitro cultures. The aim of this study was to establish a cell suspension culture of A. pichinchensis, quantify the anti-inflammatory constituents 2,3-dihydrobenzofuran (2) and 3-epilupeol (3), evaluate the anti-inflammatory potential of its extracts, and perform a phytochemical analysis. Cell suspension cultures were established in a MS culture medium of 30-g L−1 sucrose, 1.0-mg L−1 α-naphthaleneacetic acid, and 0.1-mg L−1 6-furfurylaminopurine. The ethyl acetate extract of the cell culture analyzed by gas chromatography (GC) revealed that the maximum production of anti-inflammatory compounds 2 and 3 occurs on days eight and 16, respectively, improving the time and previously reported yields in callus cultures. The anti-inflammatory activity of these extracts exhibited a significant inhibition of nitric oxide (NO) production. Furthermore, a phytochemical study of the ethyl acetate (EtOAc) and methanol (MeOH) extracts from day 20 led to the identification of 17 known compounds. The structures of the compounds were assigned by an analysis of 1D and 2D NMR data and the remainder by GC–MS. This is the first report of the production of (-)-Artemesinol, (-)-Artemesinol glucoside, encecalin, and 3,5-diprenyl-acetophenone by a cell suspension culture of A. pichinchensis.

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Section: Resultsmentioning

confidence: 99%

Establishment of a Cell Suspension Culture of Ageratina pichinchensis (Kunth) for the Improved Production of Anti-Inflammatory Compounds

Sánchez-Ramos

Álvarez

Romero-Estrada

et al. 2020

Plants

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“…Higher 'H' ion concentration induced precipitation of phosphates, gelatinization of agar and destruction of vitamins and growth regulators. Though majority of plant tissues have optimum pH from 5.0 to 5.5 (Butenko et al 1984) Arya et al (2006) found that shoot growth was well in the pH range of 4.5 to 5.8 in D. giganteus, however, best shoot multiplication rate was obtained in the medium with pH of 4.5.…”

Section: Medium Phmentioning

confidence: 99%

Limitations, progress and prospects of application of biotechnological tools in improvement of bamboo—a plant with extraordinary qualities

Singh

Singh²,

Kalia

et al. 2012

Physiol Mol Biol Plants

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Bamboos (family Poaceae) are the most beautiful and useful plants on the Earth, mainly found in the tropical and sub-tropical regions of the world. Bamboos are fast growing and early maturing, but lack of proper management of bamboo resources is leading to rapid reduction of the existing bamboosetum. Bamboo propagation through seeds is limited due to long flowering cycle of upto 120 years, seed sterility and short seed viability. Infrequent and unpredictable flowering events coupled with peculiar monocarpic behaviour i.e. flowering once before culm death, and extensive genome polyploidization are additional challenges for this woody group. Similarly, vegetative propagation by cuttings, offsets and rhizomes are also inadequate to cope up with the demand of planting stock due to large propagule size, limited availability, seasonal dependence, low multiplication rate and rooting percentage. Therefore, attempts have been made to propagate bamboos through in vitro techniques. In vitro flowering has also been achieved successfully in some bamboo species. Classification systems proposed to date need further support, as taxonomic delineation at lower levels is still lacking sufficient resolution. Tremendous advancement in molecular markers holds the promise to address the needs of bamboo taxonomy (systematics and identification) and diversity studies. Successful application of molecular marker techniques has been achieved in several bamboo species although, more studies are required to understand the population structure and genetic diversity of bamboos in a better way. In addition, some efforts have also been made to clone important genes from bamboos and also for genetic transformation using Agrobacterium and particle bombardment methods. An overview of the recent developments made in improvement of bamboos through in vitro propagation, molecular marker technologies, cloning, and transformation and transgenics has been presented. The future potential of improvement of bamboos using modern biotechnological tools has also been discussed.

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“…This fact would indicate a high capacity of the protoplasts to excrete into the medium anions rather than cations. The observed changes of pH during culture (data not shown), may be related to ammonium and nitrate utilization, or solute absorption by the mechanism of cotransport with H +, and this fact ensures the normal physiological status of protoplasts, while alkalinization was observed in the degradation phase [7].…”

Section: Discussionmentioning

confidence: 99%

Modified culture conditions for increased viability and cell wall synthesis in grapevine (Vitis vinifera L. cv. Sultanina) leaf protoplasts

Katsirdakis

Roubelakis‐Angelakis

1992

Plant Cell Tiss Organ Cult

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Studies were undertaken to determine optimum conditions for grapevine protoplast culture. Highest viability was obtained at 25 ° in the dark, and at initial pH values of 5.2 to 7.2, in the presence of 1 or 2% (w/v) sucrose and 0.6 or 0.7M mannitol or osmolality between 729 and 930mOsmolkg -~. Optimum plant growth regulators were 6-BAP/NAA at 2.3 x 10 6/10-15 × 10 -6 M, respectively. 35S-Methionine incorporation into de novo synthesized proteins for protoplasts of Nicotiana tabacum cv Xanthi (a readily regenerating species) and for the recalcitrant grapevine was studied. In tobacco the rate of protein synthesis showed 3 maxima which coincided with the distinct stages of naked protoplasts, cell wall reconstitution and induction of cell division. In grapevine protoplasts the first peak exceeded the second one, whereas no peak corresponding to the induction of cell division was observed.

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Changes in culture medium pH by cell suspension cultures of Dioscorea deltoidea

Cited by 20 publications

References 10 publications

Establishment of a Cell Suspension Culture of Ageratina pichinchensis (Kunth) for the Improved Production of Anti-Inflammatory Compounds

Establishment of a Cell Suspension Culture of Ageratina pichinchensis (Kunth) for the Improved Production of Anti-Inflammatory Compounds

Limitations, progress and prospects of application of biotechnological tools in improvement of bamboo—a plant with extraordinary qualities

Modified culture conditions for increased viability and cell wall synthesis in grapevine (Vitis vinifera L. cv. Sultanina) leaf protoplasts

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