Changes in conjugated urinary bile acids across age groups

Satô, Keiko; Kakiyama, Genta; Suzuki, Mitsuyoshi; Naritaka, Nakayuki; Takei, Hiroyuki; Sato, Hiroaki; Kimura, Akihiko; Murai, Tsuyoshi; Kurosawa, Takao; Pandak, William M.; Nittono, Hiroshi; Shimizu, Toshiaki

doi:10.1016/j.steroids.2020.108730

Cited by 10 publications

(17 citation statements)

References 34 publications

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“…Tetrahydroxy bile acids detect among urinary bile acids between seven days and one year after birth, with the highest concentration in the first month, and their concentrations stay steady from two to one year, as seen in the present study in cases of intra-hepatic and extra-hepatic cholestasis after one year of age the level of Tetrahydroxy bile acids decreases in compared to before one year old, which specifies that almost all neonates and infants with cholestasis capable of synthesizing a noteworthy amount of Tetrahydroxy bile acids, which have little toxicity, and may connects with a good outcome (Sato et al, 2020).…”

Section: Discussionsupporting

confidence: 80%

Urinary tetrahydroxy bile acids investigation in infantile cholestasis

Al-Bassam¹

2022

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Background: Tetrahydroxy bile acids present at negligible or undetectable levels in healthy human adults but present at elevated levels in urine of infants with cholestasis clinically are the most valued marker of cholestasis next to serum tests. Methods: Twenty subjects with cholestasis, age range between seven days and one year, the study measured urinary tetrahydroxy bile acids for all cases. Results: Study found the presence of urinary tetrahydroxy bile acids in new-borns and infants with infantile cholestasis.New-borns and infants with a good prognosis had higher tetrahydroxy bile acids levels than those with a poor prognosis according to their outcomes. Conclusion:The study found the incidence of urinary tetrahydroxy bile acids in new-borns and infants with infantile intra-hepatic and extra-hepatic cholestasis. Extra-hepatic cholestatic patients had higher tetrahydroxy bile acids urine level than intra-hepatic, Cytomegalovirus and idiopathic neonatal hepatitis patients had a lesser tetrahydroxy bile acids level than in patients with genetic cholestasis. Tetrahydroxy bile acids play a role in clinical managing and treatment, in addition to their possible defensive properties against hepatic injury.

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Section: Discussionsupporting

confidence: 80%

Urinary tetrahydroxy bile acids investigation in infantile cholestasis

Al-Bassam¹

2022

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“…Free bile acids can combine with glycine or taurine and form the conjugated bile acids (such as TCA, GCA and GDCA, etc.) [ 36 , 37 ]. Herein we screened the regulatory roles of these bile acids in SPX expression by using the SMMC7721 and BEL-7402 cells.…”

Section: Discussionmentioning

confidence: 99%

Mechanisms for Bile Acids CDCA- and DCA-Stimulated Hepatic Spexin Expression

Lai

Bai

et al. 2022

Cells

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Spexin (SPX) is a novel peptide involved in glucose and lipid metabolism and suppresses hepatic total bile acid levels by inhibiting hepatic cholesterol 7α-hydroxylase 1 expression. As important mediators for glycolysis/gluconeogenesis and lipid metabolism, the effects of bile acids on SPX expression is yet to be understood. By using SMMC7721 and BEL-7402 cell lines, we screened the effects of bile acids and found that chenodeoxycholic acid (CDCA) and deoxycholic acid (DCA) can stimulate SPX gene transcription. Both CDCA and DCA were able to stimulate SPX mRNA expression in the liver but not colon and ileum in mice. In SMMC7721 and BEL-7402 cells, CDCA- and DCA-induced SPX promoter activity was mimicked by bile acid receptor FXR and TGR5 activation and suppressed by FXR and TGR5 silencing. Adenylate cyclase (AC)/cyclic adenosine monophosphate (cAMP) activators significantly increased SPX promoter activity whereas the inhibitors for AC/CAMP/protein kinase A (PKA) and mitogen-activated protein kinases (MAPK) pathway attenuated CDCA- and DCA-induced SPX transcription. Thus, CDCA and DCA stimulate SPX expression at the hepatic level through FXR and TGR5 mediated AC/cAMP/PKA and MAPK cascades.

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“…BA analysis was performed according to our previous method ( 24 ). An InertSustain C18 (150 mm × 2.1 mm internal diameter, 3 μm particle size; GL Sciences Inc., Tokyo, Japan) was employed at 40°C.…”

Section: Methodsmentioning

confidence: 99%

“…An LCMS-8050 tandem mass spectrometer equipped with an ESI probe and Nexera X2 ultra-highpressure liquid chromatography system (Shimadzu Co, Kyoto Japan) was used for the following runs: BA analysis (Condition I): BA analysis was performed according to our previous method (24). An InertSustain C18 (150 mm × 2.1 mm internal diameter, 3 μm particle size; GL Sciences Inc., Tokyo, Japan) was employed at 40°C.…”

Section: Lc/ms Analysismentioning

confidence: 99%

“…There are a number of reports that have measured fecal BA profiles in healthy and diseased states such as colorectal cancer ( 19 ), liver cancer ( 20 ), ulcerative colitis ( 21 ), cirrhosis ( 22 ), and in centenarians ( 23 ). Although recent advances in LC/MS technology have dramatically improved BA profiling ( 24 ), the presence of many isomers as well as various types of conjugated forms still complicates the separation and detection of fecal BAs. In fact, esterified BAs are routinely omitted from the measurements.…”

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confidence: 99%

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