Changes in complement responses in Gilthead seabream (Sparus aurata) and European seabass (Dicentrarchus labrax) under crowding stress, plus viral and bacterial challenges

Mauri, Isabella; Romero, Alejandro; Acerete, Laura; Mackenzie, Simon; Roher, Nerea; Callol, A.; Cano, Irene; Álvarez, M. Carmen; Tort, L.

doi:10.1016/j.fsi.2010.10.006

Cited by 83 publications

(53 citation statements)

References 37 publications

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“…Furthermore, the option that mucus proteins are not produced by epidermal cells can be also considered but needs further confirmation. Our results are in an agreement with a previous study which demonstrated that overcrowding stress did not modify c3 mRNA levels in liver from gilthead seabream [67].…”

Section: Accepted M Manuscriptsupporting

confidence: 94%

Differential proteome profile of skin mucus of gilthead seabream (Sparus aurata) after probiotic intake and/or overcrowding stress

Cordero¹,

Morcillo

Cuesta

et al. 2016

Journal of Proteomics

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Differential proteome profile of skin mucus of gilthead seabream (sparus aurata) after probiotic intake and/or overcrowding stress, Journal of Proteomics (2015Proteomics ( ), doi: 10.1016Proteomics ( /j.jprot.2015 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A C C E P T E DM A N U S C R I P T ACCEPTED MANUSCRIPT A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT2 AbstractGilthead seabream (Sparus aurata L.) is the major cultured fish species in the Mediterranean area. High density stocking causes stress and increases the impact of diseases leading to economic losses. Probiotics could represent a solution to prevent diseases through several mechanisms such as improving the immune status and/or mucosal microbiota or competing with pathogens. The probiotic Shewanella putrefaciens, also known as Pdp11, was firstly isolated from the skin of healthy gilthead seabream. Our study focuses on the skin mucus proteome after dietary probiotic Pdp11 intake in fish maintained under normal or overcrowding conditions. 2-DE of skin mucus followed by LC-MS/MS analysis was done for each experimental group and differentially expressed proteins were identified. The results showed differentially expressed proteins especially involved in immune processes, such as lysozyme, complement C3, natural killer cell enhancing factor and nonspecific cytotoxic cell receptor protein 1, whose transcript profiles were studied by qPCR. A consistency between lysozyme protein levels in the mucus and lysozyme mRNA levels in skin were found. Further research is necessary to unravel the implications of skin mucosal immunity on fish welfare and disease. Biological significanceThe present work reveals the proteomic changes, which are taking place in the skin mucus of stressed and non-stressed gilthead seabream after Pdp11 probiotic intake. The study contributes to improve the knowledge on skin mucosal immunology of this relevant farmed fish species.Furthermore, the paper shows for the first time how a suitable proteomic methodology, in this case 2-DE followed by LC-MS/MS is useful to perform a comparative study with a noninvasive technique of skin mucus of gilthead seabream.

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Section: Accepted M Manuscriptsupporting

confidence: 94%

Differential proteome profile of skin mucus of gilthead seabream (Sparus aurata) after probiotic intake and/or overcrowding stress

Cordero¹,

Morcillo

Cuesta

et al. 2016

Journal of Proteomics

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“…6 shows that viral treatment or high density plus infection would differentially upregulate esbC3_2 isoform whereas the esbC3_1 isoform would be upregulated under non-specific challenges/stressors, since either high density or bacterial exposure induce differentially its expression. In previous works such stress-induced complement activation was already observed [62,63], and it was suggested that the immuneendocrine connection could play a role, and in particular hormones such as cortisol. In the present work, although cortisol levels could not be analyzed in all experimental animals, the available data reveals that both high density and challenge increased plasma cortisol by a rate of 20e40 fold the basal levels, which is in accordance with previous results on in vitro and in vivo stress challenges in fish species [64e67].…”

Section: Discussionmentioning

confidence: 85%

Molecular cloning and characterization of European seabass (Dicentrarchus labrax) and Gilthead seabream (Sparus aurata) complement component C3

Mauri

Roher

Mackenzie

et al. 2011

Fish & Shellfish Immunology

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“…In addition, common aquaculture practices may include long term chronic stressors and non optimum rearing conditions like unpreferable stocking densities, water quality deterioration, unfavorable social hierarchies, exposure to pathogens which affect the physiological stress response and may lead to severe negative consequences on performance, disease resistance and welfare (Ashley, 2007;Barton and Iwama, 1991;Conte, 2004;Di Marco et al, 2008;Ellis et al, 2002;Mauri et al, 2011;Pickering, 1993;Sammouth et al, 2009;Schreck, 1981;Sumpter, 1993;Wendelaar Bonga, 1997).…”

Section: Discussionmentioning

confidence: 99%

Early life stress and effects at subsequent stages of development in European sea bass (D. labrax)

2015

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Changes in complement responses in Gilthead seabream (Sparus aurata) and European seabass (Dicentrarchus labrax) under crowding stress, plus viral and bacterial challenges

Cited by 83 publications

References 37 publications

Differential proteome profile of skin mucus of gilthead seabream (Sparus aurata) after probiotic intake and/or overcrowding stress

Differential proteome profile of skin mucus of gilthead seabream (Sparus aurata) after probiotic intake and/or overcrowding stress

Molecular cloning and characterization of European seabass (Dicentrarchus labrax) and Gilthead seabream (Sparus aurata) complement component C3

Early life stress and effects at subsequent stages of development in European sea bass (D. labrax)

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