Changes in Chemical Structure of n-Acyl Homoserine Lactones and Their Effects on Microcystin Expression from <i>Microcystis aeruginosa</i> PCC7806

Samanamud, Gisella Rossana Lamas; Reeves, Tony E.; Tidwell, Michael; Bohmann, Jonathan; Lange, Kenneth; Shipley, Heather J.

doi:10.1089/ees.2021.0089

Cited by 9 publications

(6 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There was a concern that the small volume used in these experiments would be affected by evaporation, and this must be considered. However, previous experiments conducted in a similar way [ 42 , 43 ] were also affected by the evaporation rate, which was not measured in this experiment. Future studies can consider different types of inoculation volumes and compare these results statistically to those found in this study.…”

Section: Resultsmentioning

confidence: 75%

“…In fact, several studies have determined the importance of these genes in toxin production [ 31 , 32 , 33 , 34 , 35 , 36 , 37 ]. A preliminary study [ 42 ] revealed that even if you have a depletion of other genes, there is still a chance of finding microcystin. However, when there is the depletion of mcyB , there is little or no microcystin production.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

The Role of Quorum Sensing in the Development of Microcystis aeruginosa Blooms: Gene Expression

et al. 2023

Self Cite

View full text Add to dashboard Cite

Microcystis aeruginosa (M. aeruginosa) is the dominant cyanobacterial species causing harmful algal blooms in water bodies worldwide. The blooms release potent toxins and pose severe public health hazards to water bodies, animals, and humans who are in contact with or consume this water. The interaction between M. aeruginosa and heterotrophic bacteria is thought to contribute to the development of the blooms. This study strives to provide a specific answer to whether quorum sensing is also a potential mechanism mediating the interaction of different strains/species and the expression by gene luxS or gene mcyB in M. aeruginosa growth. The luxS gene in M. aeruginosa PCC7806 is associated with quorum sensing and was tested by q-PCR throughout a 30-day growth period. The same was performed for the mcyB gene. Heterotrophic bacteria were collected from local water bodies: Cibolo Creek and Leon Creek in San Antonio, Texas. Results revealed that in algal bloom scenarios, there is a similar concentration of gene luxS that is expressed by the cyanobacteria. Gene mcyB, however, is not directly associated with algal blooms, but it is related to cyanotoxin production. Toxicity levels increased in experiments with multiple algal strains, and the HSL treatment was not effective at reducing microcystin levels.

show abstract

Section: Resultsmentioning

confidence: 75%

Section: Resultsmentioning

confidence: 99%

The Role of Quorum Sensing in the Development of Microcystis aeruginosa Blooms: Gene Expression

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…Readily identifiable homologues of the LuxI/LuxR family are not encoded in most sequenced cyanobacterial genomes . Nonetheless, AHLs are routinely found as extracellular metabolites in microbial communities dominated by cyanobacteria, such as cyanobacterial blooms, , and exogenously added AHL signals have been shown to alter growth or other physiological characteristics in a limited number of axenic cyanobacterial cultures. − The cyanobacterial species Microcystis aeruginosa and Gloeothece sp. PCC 6909 have been shown to directly secrete some AHL molecules, , although their physiological function is poorly understood.…”

Section: Resultsmentioning

confidence: 99%

Developing Cyanobacterial Quorum Sensing Toolkits: Toward Interspecies Coordination in Mixed Autotroph/Heterotroph Communities

et al. 2022

View full text Add to dashboard Cite

There has been substantial recent interest in the promise of sustainable, light-driven bioproduction using cyanobacteria, including developing efforts for microbial bioproduction using mixed autotroph/heterotroph communities, which could provide useful properties, such as division of metabolic labor. However, building stable mixed-species communities of sufficient productivity remains a challenge, partly due to the lack of strategies for synchronizing and coordinating biological activities across different species. To address this obstacle, we developed an inter-species communication system using quorum sensing (QS) modules derived from well-studied pathways in heterotrophic microbes. In the model cyanobacterium, Synechococcus elongatus PCC 7942 (S. elongatus), we designed, integrated, and characterized genetic circuits that detect acyl-homoserine lactones (AHLs), diffusible signals utilized in many QS pathways. We showed that these receiver modules sense exogenously supplied AHL molecules and activate gene expression in a dose-dependent manner. We characterized these AHL receiver circuits in parallel with Escherichia coli W (E. coli W) to dissect species-specific properties, finding broad agreement, albeit with increased basal expression in S. elongatus. Our engineered “sender” E. coli strains accumulated biologically synthesized AHLs within the supernatant and activated receiver strains similarly to exogenous AHL activation. Our results will bolster the design of sophisticated genetic circuits in cyanobacterial/heterotroph consortia and the engineering of QS-like behaviors across cyanobacterial populations.

show abstract

“…3-OH-C4-HSL BG-11 [42] M. aeruginosa PCC7806 C3-HSL BG-11 C4-HSL BG-11 [43] M. aeruginosa FACHB 905 AHLs(C13H19O8N) BG-11…”

Section: Articlementioning

confidence: 99%

Cyanobacterial Blooms Formation by Enhanced Ecological Adaptability and Competitive Advantage of <em>Microcystis</em>— Non-Negligible Role of Quorum-Sensing

Zhang,

2024

Preprint

View full text Add to dashboard Cite

Microcystis-dominated cyanobacterial blooms (MCBs) frequently occur in freshwaters worldwide due to massive Microcystis colonies formation and severely threaten human and ecosystems health. Quorum sensing (QS) is a direct cause of Microcystis colonies formation, possibly regulating MCBs occur and Microcystis population behaviors [1, 2]. Many novel findings in fundamental knowledge of Microcystis QS phenomenon and the signaling molecules have been documented. However, little effort has devoted to comprehensively summarize and discuss research progresses and exploration direction on QS signaling molecules-mediated QS system in Microcystis. This article summarizes action process of N-acyl homoserine lactones (AHLs) as major signaling molecules in Microcystis and discussed the detailed roles of AHLs-mediated QS system in cells aggregation, colonies formation, ecological adaptability and competitive advantage. And the progress was summarized in research on the QS mechanism in Microcystis. Compared to other QS system, the LuxI/LuxR-type QS system is more likely to found in Microcystis. Also, we introduced Quorum quenching (QQ) — QS blocking process in Microcystis, with emphasizing on the potential of QS inhibitors in MCBs control. Finally, in response to the research deficiencies and gaps in Microcystis QS and QQ, we proposed several future research directions in these fields. This review may deepen the understanding of Microcystis QS knowledge and provide theoretical guidance for the development of strategies to monitor, control and harness MCBs.

show abstract

Changes in Chemical Structure of n-Acyl Homoserine Lactones and Their Effects on Microcystin Expression from Microcystis aeruginosa PCC7806

Cited by 9 publications

References 46 publications

The Role of Quorum Sensing in the Development of Microcystis aeruginosa Blooms: Gene Expression

The Role of Quorum Sensing in the Development of Microcystis aeruginosa Blooms: Gene Expression

Developing Cyanobacterial Quorum Sensing Toolkits: Toward Interspecies Coordination in Mixed Autotroph/Heterotroph Communities

Cyanobacterial Blooms Formation by Enhanced Ecological Adaptability and Competitive Advantage of <em>Microcystis</em>— Non-Negligible Role of Quorum-Sensing

Contact Info

Product

Resources

About