Changes in cellular thiol content and intracellular Zn2+ level by 1,4-naphthoquinone in rat thymocytes

Fukunaga, Eri; Ishida, Shiro; Oyama, Yasuo

doi:10.1016/j.cbi.2014.08.007

Cited by 5 publications

(7 citation statements)

References 36 publications

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“…NAPH causes in vitro and in vivo toxicities (Wilson et al, 1996;Lamé et al, 2003;Ishihara et al, 2011) via the formation of reactive oxygen species (Kumar et al, 2009;Srinivas et al, 2011). In our previous study (Fukunaga et al, 2014), we showed that NAPH at sublethal concentrations increased the intracellular Zn 2+ levels in rat thymocytes. This phenomenon is one of the common features of cells affected by oxidative stress because the conversion of thiols to disulfides by oxidative stress releases Zn 2+ (Maret, 1994), resulting in an increase in intracellular Zn 2+ levels (Kinazaki et al, 2011).…”

Section: Introductionmentioning

confidence: 74%

“…In our previous study, NAPH significantly increased cell lethality at a concentration ≥ 3 μM (Fukunaga et al, 2014). The experiments in the current study were carried out with sublethal concentrations (0.1-1 μM) of NAPH.…”

Section: The Effect Of Naph On the H 2 O 2 -Induced Increase In Cell mentioning

confidence: 92%

“…The simultaneous incubation with NAPH and H 2 O 2 increased the fluorescence to a lesser extent compared to that with H 2 O 2 alone. Both NAPH and H 2 O 2 are known to increase intracellular Zn 2+ levels in rat thymocytes (Matsui et al, 2010;Fukunaga et al, 2014). If the elevation of the intracellular Zn 2+ level would disturb the binding of Ca 2+ to Fluo-3 because of the higher affinity of Zn 2+ to Fluo-3, it would attenuate the augmentation of the Fluo-3 fluorescence.…”

Section: (B) (A)mentioning

confidence: 99%

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The paradoxical effect of 1,4-naphthoquinone on the process of cell death induced by hydrogen peroxide in rat thymocytes

Ohtani

Fukunaga

Oyama

et al. 2014

Fundam. Toxicol. Sci.

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-1,4-Naphthoquinone (NAPH) is found in diesel exhaust particles and it is an active metabolite of naphthalene, a fumigant insecticide. This compound is known to cause oxidative stress. Therefore, it is plausible to suggest that NAPH increases cell vulnerability to oxidative stress in an additive or synergistic manner. We tested this possibility using rat thymocytes with flow-cytometric techniques and appropriate fluorescent probes. NAPH attenuated the increase in cell lethality induced by hydrogen peroxide (H 2 O 2). The combination of NAPH and H 2 O 2 promoted the transition from normal cells to apoptotic living cells, but attenuated further transition to cell death. Thus, the process of cell death induced by H 2 O 2 was not completed in the presence of NAPH. However, NAPH did not attenuate certain lethal cellular events such as decrease in the cellular content of non-protein thiols and increases in intracellular Ca 2+ and Zn 2+ levels, induced by H 2 O 2. The inhibitory effect of NAPH on the increase in cell lethality induced by H 2 O 2 was also observed when caspase activity was suppressed. In the present study, the mechanism underlying the NAPH-induced attenuation of cell death in cells affected by H 2 O 2-generated oxidative stress was, however, not fully elucidated. Since both H 2 O 2 and NAPH elevated intracellular Ca 2+ and Zn 2+ levels, and since Zn 2+ is known to partly attenuate Ca 2+-dependent cell death, the intracellular interaction between Ca 2+ and Zn 2+ may complicate the process of cell death induced by oxidative stress.

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Section: Introductionmentioning

confidence: 74%

Section: The Effect Of Naph On the H 2 O 2 -Induced Increase In Cell mentioning

confidence: 92%

Section: (B) (A)mentioning

confidence: 99%

See 1 more Smart Citation

The paradoxical effect of 1,4-naphthoquinone on the process of cell death induced by hydrogen peroxide in rat thymocytes

Ohtani

Fukunaga

Oyama

et al. 2014

Fundam. Toxicol. Sci.

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“…However, some cautions may be necessary to interpret Zn 2+ -mediated actions. We showed that small increase in intracellular Zn 2+ concentrations increased cellular content of nonprotein thiols [14,28,42]. The Zn 2+ -induced increase in cellular content of nonprotein thiols (glutathione) by chemical compounds may be cytoprotective against oxidative stress.…”

Section: Toxicological Implicationsmentioning

confidence: 84%

“…Zn 2+ influx into cells under control conditions was significantly reduced at low temperatures [14]. To determine if the Ziram-induced influx of Zn 2+ is sensitive to temperature, the effect of 0.3-1 lM Ziram on FluoZin-3 fluorescence was examined under cold conditions (3-4°C).…”

Section: Ziram-induced Augmentation Of Fluozin-3 Fluorescence Under Cmentioning

confidence: 99%

Dithiocarbamate fungicides increase intracellular Zn2+ levels by increasing influx of Zn2+ in rat thymic lymphocytes

Kanemoto-Kataoka

Oyama

Ishibashi

et al. 2015

Chemico-Biological Interactions

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Modification of cell vulnerability to oxidative stress by N-(3-oxododecanoyl)-L-homoserine-lactone, a quorum sensing molecule, in rat thymocytes

Nishimura-Danjobara¹,

Oyama

et al. 2019

Chemico-Biological Interactions

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Changes in cellular thiol content and intracellular Zn2+ level by 1,4-naphthoquinone in rat thymocytes

Cited by 5 publications

References 36 publications

The paradoxical effect of 1,4-naphthoquinone on the process of cell death induced by hydrogen peroxide in rat thymocytes

The paradoxical effect of 1,4-naphthoquinone on the process of cell death induced by hydrogen peroxide in rat thymocytes

Dithiocarbamate fungicides increase intracellular Zn2+ levels by increasing influx of Zn2+ in rat thymic lymphocytes

Modification of cell vulnerability to oxidative stress by N-(3-oxododecanoyl)-L-homoserine-lactone, a quorum sensing molecule, in rat thymocytes

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