Changes in cell wall structure and protein set in Candida maltosa grown on hexadecane

Zvonarev, Anton; Farofonova, Vasilina; Kulakovskaya, Ekaterina; Kulakovskaya, T. V.; Мачулин, А. В.; Sokolov, Svyatoslav S.; Дмитриев, В. В.

doi:10.1007/s12223-020-00840-2

Cited by 4 publications

(9 citation statements)

References 36 publications

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“…Electron microscopic analysis of the cell surface and their environment during growth on glucose revealed as expected nothing unusual (Figure 2a-c). The cells utilizing hexadecane had modified cell wall zones on the surface (canals) similar to observed earlier in hexadecane-grown C. maltosa [20][21][22][23]. The presence of extracellular vesicles was also confirmed, as well as with fluorescence microscopy.…”

Section: Fluorescent and Scanning Electron Microscopy Of Yeasts Cellssupporting

confidence: 80%

“…An increase in the content of the acid-insoluble fraction was observed earlier for another strain of C. maltosa cultivated on hexadecane [23].…”

Section: Polyphosphate Content In the Cells Cultivated On Glucose Or ...supporting

confidence: 74%

“…contribution to the increase in the content of polyP was made by the acid-insoluble fraction (Table 1). An increase in the content of the acid-insoluble fraction was observed earlier for another strain of C. maltosa cultivated on hexadecane [23].…”

Section: The Treatment With Polyphosphatasesupporting

confidence: 74%

“…In addition, it has been found that similar structures are also formed in the yeast cell wall during carbon starvation. Electron microscopic and biochemical studies revealed for the first time the formation of canals in the cell wall during the growth on hydrocarbons [ 20 , 21 , 22 , 23 ]. The unique properties of canals—substrate dependence, hydrophobicity, high concentration of oxidizing enzymes and strong chemical and mechanical connection with other components of the cell wall—indicate that these structures are the key components of metabolic interaction with the environment.…”

Section: Introductionmentioning

confidence: 99%

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The Extracellular Vesicles Containing Inorganic Polyphosphate of Candida Yeast upon Growth on Hexadecane

Zvonarev,

Trilisenko,

Farofonova

et al. 2023

JoX

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The cell wall of Candida yeast grown on presence of hexadecane as a sole carbon source undergoes structural and functional changes including the formation of specific supramolecular complexes—canals. The canals contain specific polysaccharides and enzymes that provide primary oxidization of alkanes. In addition, inorganic polyphosphate (polyP) was identified in Candida maltosa canals. The aim of the work was a comparative study of the features of cell walls and extracellular structures in yeast C. maltosa, C. albicans and C. tropicalis with special attention to inorganic polyphosphates as possible part of these structures when grown on the widely used xenobiotic hexadecane (diesel fuel). Fluorescence microscopy with DAPI has shown an unusual localization of polyP on the cell surface and in the exovesicles in the three yeast species, when growing on hexadecane. Electron-scanning microscopy showed that the exovesicles were associated with the cell wall and also presented in the external environment probably as biofilm components. Treatment of hexadecane-grown cells with purified Ppx1 polyphosphatase led to the release of phosphate into the incubation medium and the disappearance of polyP in vesicles and cell wall observed using microscopic methods. The results indicate the important role of polyP in the formation of extracellular structures in the Candida yeast when consuming hexadecane and are important for the design of xenobiotic destructors based on yeast or mixed cultures.

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Section: Fluorescent and Scanning Electron Microscopy Of Yeasts Cellssupporting

confidence: 80%

“…An increase in the content of the acid-insoluble fraction was observed earlier for another strain of C. maltosa cultivated on hexadecane [23].…”

Section: Polyphosphate Content In the Cells Cultivated On Glucose Or ...supporting

confidence: 74%

Section: The Treatment With Polyphosphatasesupporting

confidence: 74%

Section: Introductionmentioning

confidence: 99%

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The Extracellular Vesicles Containing Inorganic Polyphosphate of Candida Yeast upon Growth on Hexadecane

Zvonarev,

Trilisenko,

Farofonova

et al. 2023

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“…Some species, such as Y. lipolytica , are known to produce biosurfactants to overcome those limitations (Soong et al, 2019 ; Yalçın et al, 2018 ). Physiological changes have been documented in other species, such as C. maltosa , to improve contact with and utilization of hydrophobic carbon sources (Zvonarev et al, 2021 ). Some of the other strains could be employing similar mechanisms, but uptake mechanisms for hydrophobic substrates are poorly characterized for the yeast species studied here.…”

Section: Resultsmentioning

confidence: 99%

Survey of nonconventional yeasts for lipid and hydrocarbon biotechnology

Ocasio

Khalid

Truka

et al. 2022

Journal of Industrial Microbiology and Biotechnology

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Non-conventional yeasts have an untapped potential to expand biotechnology and enable process development necessary for a circular economy. They are especially convenient for the field of lipid and hydrocarbon biotechnology because they offer faster growth than plants, easier scalability than microalgae and exhibit increased tolerance relative to some bacteria. The ability of industrial organisms to import and metabolically transform lipids and hydrocarbons are crucial in such applications. Here, we assessed the ability of 14 yeasts to utilize 18 model lipids and hydrocarbons from six functional groups and three carbon chain lengths. The studied strains covered 12 genera from nine families. Nine non-conventional yeast performed better than Saccharomyces cerevisiae, the most common industrial yeast. Rhodotorula toruloides, Candida maltosa, Scheffersomyces stipis and Yarowia lipolytica were observed to grow significantly better and on more types of lipid and lipid-molecules than other strains. They were all able to utilize mid to long-chain fatty acids, fatty alcohols, alkanes, alkenes and dicarboxylic acids, including 28 previously unreported substrates across the four yeasts. Interestingly, a phylogenetic analysis showed a short evolutionary distance between the R. toruloides and C. maltosa and S. stipitis, even though R. toruloides is classified under a different phylum. This work provides valuable insight into the lipid substrate range of non-conventional yeasts that can inform species selection decisions and viability of lipid feedstocks.

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Mycoremediation of n-alkanes under aerobic conditions – A review

Palmgren,

Ivarsson

2024

Fungal Interactions

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Changes in cell wall structure and protein set in Candida maltosa grown on hexadecane

Cited by 4 publications

References 36 publications

The Extracellular Vesicles Containing Inorganic Polyphosphate of Candida Yeast upon Growth on Hexadecane

The Extracellular Vesicles Containing Inorganic Polyphosphate of Candida Yeast upon Growth on Hexadecane

Survey of nonconventional yeasts for lipid and hydrocarbon biotechnology

Mycoremediation of n-alkanes under aerobic conditions – A review

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