Changes in CD4+CD8–/CD4–CD8+ Ratio and Humoral Immune Functions in Vitamin B12-Deficient Rats

Funada, Ulala; Wada, Masahiro; Kawata, Toshitsugu; Mori, Kazumi; Tamai, Hiroko; Kawanishi, Tomiko; Kunou, Akiko; Tanaka, Nobuo; Tadokoro, Tadahiro; Maekawa, Akio

doi:10.1024/0300-9831.70.4.167

Cited by 18 publications

(17 citation statements)

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“…Two studies reported that serum C3, IgG, and IgM levels declined in vitamin B 12 -deficient rats and this decline was restored after vitamin B 12 administration [14,15] . Our patients also showed a significant increase in serum levels of C3, IgG, IgA, and IgM, and a slight elevation in C4 level after vitamin B 12 treatment.…”

Section: Discussionmentioning

confidence: 99%

Effects of Cyanocobalamin on Immunity in Patients with Pernicious Anemia

Erkurt

Aydoğdu

Dikilitaş³

et al. 2008

Med Princ Pract

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Objective: The aim of the study was to evaluate the role of vitamin B₁₂ in patients with pernicious anemia. Materials and Methods: This study was conducted prospectively at the Turgut Özal Medical Center, Department of Hematology, between April and November 2002. Absolute numbers and ratio of the surface antigens of T and B lymphocyte subgroups, CD4/CD8 ratio were calculated in order to evaluate changes in leukocyte and lymphocyte numbers; natural killer (NK) cell count, serum C3, C4, and levels of immunoglobulins G, A, and M were also measured to evaluate vitamin B₁₂ effect on immunity. Values obtained before treatment with cyanocobalamin were compared with those found during peak reticulocyte count. Results: In vitamin B₁₂-deficient patients, absolute numbers of CD4+ and especially CD8+ lymphocytes were found to be decreased; CD4/CD8 ratio increased, and NK cell activity was depressed. After cyanocobalamin treatment, absolute numbers and percentage of lymphocyte subgroups were elevated. Increased CD4/CD8 ratio and depressed NK cell activity were restored and levels of C3, C4, and immunoglobulins were elevated. Conclusion: These findings suggest that vitamin B₁₂ has important immunomodulatory effects on cellular immunity, and abnormalities in the immune system in pernicious anemia are restored by vitamin B₁₂ replacement therapy.

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Section: Discussionmentioning

confidence: 99%

Effects of Cyanocobalamin on Immunity in Patients with Pernicious Anemia

Erkurt

Aydoğdu

Dikilitaş³

et al. 2008

Med Princ Pract

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“…Vitamin A deficiency has also been shown to reduce the CD4 ϩ cell proportion in humans (39). A few in vivo studies showed a decrease in CD8 ϩ cell population with deficiencies in vitamin D (40), vitamin E (41), and vitamin B12 (42)(43)(44). Interestingly, vitamin B12 plays an important role in DNA metabolism and B12 deficiency can cause uracil misincorporation into DNA by the same mechanism as folate deficiency…”

Section: Discussionmentioning

confidence: 99%

Folate Deficiency Inhibits the Proliferation of Primary Human CD8+ T Lymphocytes In Vitro

Courtemanche

Elson-Schwab

Mashiyama

et al. 2004

The Journal of Immunology

148

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Folate is required for one-carbon transfer reactions and the formation of purines and pyrimidines for DNA and RNA synthesis. Deficiency of folate can lead to many clinical abnormalities, including macrocytic anemia, cardiovascular diseases, birth defects, and carcinogenesis. The nucleotide imbalance due to folate deficiency causes cell cycle arrest in the S phase and uracil misincorporation into DNA, which may result in DNA double-strand breaks during repair. The role of folate in the immune system has not been fully characterized. We cultured PHA-activated human T lymphocytes in varying concentrations of folate, and measured proliferation, cell cycle, apoptosis, uracil misincorporation, and proportions of Th cells (CD4+) and cytotoxic T (CD8+) cells. Folate deficiency reduced proliferation of T lymphocytes, induced cell cycle arrest in the S phase, induced apoptosis, and increased the level of uracil in DNA. Folate deficiency also increased the CD4+ to CD8+ ratio due to a marked reduction of CD8+ cell proliferation. Folate or nucleoside repletion of folate-deficient cells rapidly restored T lymphocyte proliferation and normal cell cycle, reduced the DNA uracil content, and lowered the CD4+ to CD8+ ratio. These data suggest that folate status may affect the immune system by reducing the capacity of CD8+ cells to proliferate in response to activation.

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“…Nutritional status was assessed by anthropometry and by the presence of signs of nutritional deficiencies (51) . Due to funding limitations, serum biochemical analyses for vitamins A, B 12 , riboflavin, Fe and Zn were performed only at baseline and the end of year one for Cohort I. Analytical methods used have been fully described previously (52) . Stool samples were examined for intestinal ova and parasites using the formal-ether sedimentation method and Lugol's stain (53) .…”

Section: Child Measuresmentioning

confidence: 99%

“…Children with severe protein-energy malnutrition often have high case fatality rates, particularly from measles, pneumonia, diarrhoea, malaria and tuberculosis (2,4) . Deficiencies of Fe, Zn, vitamin A and vitamin B 12 can impair cell-mediated immunity and granulocyte function, resulting in disruption of anatomic barriers to pathogens (10)(11)(12)(13)(14) . Multiple micronutrient fortification of milk has been shown to reduce instances of pneumonia, diarrhoea, measles and high fever (15) .…”

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confidence: 99%

“…Fesupplemented pre-school children (aged 1-35 months) in an area with endemic malaria showed an increased risk of death or hospitalization from malarial adverse events (43) . A wide variety of ASF (including blood, offal, fish, insects and molluscs) contain energy, complete protein and micronutrients such as bioavailable Fe and Zn, vitamin B 12 and preformed vitamin A (5) . Several food-based ASF interventions have demonstrated reductions in morbidity, namely fever, respiratory and diarrhoeal infections and anaemia (44)(45)(46) .…”

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confidence: 99%

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School snacks decrease morbidity in Kenyan schoolchildren: a cluster randomized, controlled feeding intervention trial

Neumann

Bwibo

Jiang

et al. 2013

Public Health Nutr.

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Objective: To examine the effects of three different school snacks on morbidity outcomes. Design: Twelve schools were randomized to either one of three feeding groups or a Control group. There were three schools per group in this cluster randomized trial. Children in feeding group schools received school snacks of a local plant-based dish, githeri, with meat, milk or extra oil added. The oil used was later found to be fortified with retinol. Physical status, food intake and morbidity outcomes were assessed longitudinally over two years. Setting: Rural Embu District, Kenya, an area with high prevalence of vitamin A deficiency. Subjects: Standard 1 schoolchildren (n 902; analytic sample) enrolled in two cohorts from the same schools one year apart. Results: The Meat and Plain Githeri (i.e. githeri 1 oil) groups showed the greatest declines in the probability of a morbidity outcome (PMO) for total and severe illnesses, malaria, poor appetite, reduced activity, fever and chills. The Meat group showed significantly greater declines in PMO for gastroenteritis (mainly diarrhoea) and typhoid compared with the Control group, for jaundice compared with the Plain Githeri group, and for skin infection compared with the Milk group. The Milk group showed the greatest decline in PMO for upper respiratory infection. For nearly all morbidity outcomes the Control group had the highest PMO and the least decline over time. Conclusions: The intervention study showed beneficial effects of both animalsource foods and of vitamin A-fortified oil on morbidity status. KeywordsKenya Supplemental school feeding Randomized controlled trial Morbidity Infection Schoolchildren Globally, the negative health consequences of malnutrition and infection are a leading cause of morbidity and mortality in children, contributing to 53 % of all deaths in children under 5 years of age (1)(2)(3)(4) . Malnutrition is estimated to contribute to 45 % of measles deaths, 52 % in pneumonia, 57 % in malaria and 61 % in diarrhoea (1)(2)(3)(4)

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Changes in CD4⁺CD8^–/CD4^–CD8⁺ Ratio and Humoral Immune Functions in Vitamin B₁₂-Deficient Rats

Cited by 18 publications

References 0 publications

Effects of Cyanocobalamin on Immunity in Patients with Pernicious Anemia

Effects of Cyanocobalamin on Immunity in Patients with Pernicious Anemia

Folate Deficiency Inhibits the Proliferation of Primary Human CD8+ T Lymphocytes In Vitro

School snacks decrease morbidity in Kenyan schoolchildren: a cluster randomized, controlled feeding intervention trial

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