Changes in CD34+ cell count in peripheral blood after whole blood donation

Pala, Çiğdem; Mumcuoglu, Haluk; Kurnaz, Fatih; Şıvgın, Serdar; Kaynar, Leylagül; Keklik, Muzaffer; Akyol, Gulsah; Koker, Mustafa Yavuz; Eser, Bülent

doi:10.1016/j.transci.2013.04.038

Cited by 3 publications

(2 citation statements)

References 5 publications

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“…Previous studies have majorly evaluated the effects of regular blood donation on hematopoiesis through the measurement of iron-associated blood parameters and have introduced iron deficiency as its main side effect ( 18 , 25 ). Although one study has reported that there is no significant difference between the number of CD34 + cells isolated from donors before and immediately after a blood donation, it was a short-term study and could not predict long-term effects of blood donation ( 26 ). Here, we analyzed the data related to donors whose health was determined by regular examinations in the transfusion centers before donation and showed that there was 1.19-fold reduction in the number of CD34 + cells from the regular donors while the myeloid to leucocyte (M/L) ratio did not significantly change ( Table 3 ).…”

Section: Discussionmentioning

confidence: 99%

Long-term Effects of Regular Whole Blood Donation on Peripheral Blood CD34+ Cells Population with using Leukoreduction Filters

et al. 2022

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Scan to discover onlineBackground & Objective: Trapped cell population in leukoreduction filters (LRFs) contains such a significant number of CD34 + hematopoietic stem cells that can be recovered to be used in research studies.Methods: Samples (n=20) were obtained from 10 first-time donors and 10 regular blood donors with more than 30 times blood donation. After separating leukocytes from LRFs by backflushing, total leukocyte number and differential count were determined in both groups using an automated haemocytometer. Then cell viability and CD34 + cell quantification were assessed using 7-amino-actinomycin D and fluorescent-labeled monoclonal antibodies using flow cytometry, respectively.Results: Total leukocyte count was 665±164.92×10 6 in the first-time blood donors and 883±233.89×10 6 in the regular donors, which were not significantly different (P=0.08). While the number of CD34 + cells was significantly reduced in the regular donors compared to the first-time donors (0.58±0.20×10 6 /µL vs. 0.36±0.22×10 6 /µL; P=0.034). There was no significant difference in terms of absolute neutrophil count (10.58±3.66×0 6 vs. 13.17±6.45×10 6 /µL; P=0.349), lymphocytes (7.75±3.11×10 6 vs. 10.38±3.77×10 6 /µL; P=0.917), and monocytes (2.31±0.88×10 6 vs. 2.59±1.09×10 6 /µL; P=0.591) between the first-time and regular donor groups, respectively. Based on the correlation coefficients, the participants' age had no significant effect on these variables. Conclusion:The results of this study depicted that regular blood donation reduces the number of CD34 + cells in the peripheral blood (PB) of regular donors while it has no significant effect on the ratio of myeloid to lymphoid cells of the two groups.

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Section: Discussionmentioning

confidence: 99%

Long-term Effects of Regular Whole Blood Donation on Peripheral Blood CD34+ Cells Population with using Leukoreduction Filters

et al. 2022

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“…Changes in lymphocyte subsets have been observed early following blood donation. For example, human stem cells (HSCs), the principal marker of which is CD34, increased significantly at 6 h following blood donation and returned to baseline at 24 h [6]. CD16-56 (NK) cells have been observed to decrease following blood donation [7].…”

Section: Introductionmentioning

confidence: 99%

Changes in hematological indices and lymphocyte subsets in response to whole blood donation in healthy male donors

Borai

Livingstone

Alsobhi

et al. 2017

Scandinavian Journal of Clinical and Laboratory Investigation

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Whole blood donation has immunomodulatory effects, and most of these have been observed at short intervals following blood donation. This study aimed to investigate the impact of whole blood donation on lymphocyte subsets over a typical inter-donation interval. Healthy male subjects were recruited to study changes in complete blood count (CBC) (n = 42) and lymphocyte subsets (n = 16) before and at four intervals up to 106 days following blood donation. Repeated measures ANOVA were used to compare quantitative variables between different visits. Following blood donation, changes in CBC and erythropoietin were as expected. The neutrophil count increased by 11.3% at 8 days (p < .001). Novel changes were observed in lymphocyte subsets as the CD4/CD8 ratio increased by 9.2% (p < .05) at 8 days and 13.7% (p < .05) at 22 days. CD16-56 cells decreased by 16.2% (p < .05) at 8 days. All the subsets had returned to baseline by 106 days. Regression analysis showed that the changes in CD16-56 cells and CD4/CD8 ratio were not significant (Wilk's lambda = 0.15 and 0.94, respectively) when adjusted for BMI. In conclusion, following whole blood donation, there are transient changes in lymphocyte subsets. The effect of BMI on lymphocyte subsets and the effect of this immunomodulation on the immune response merit further investigation.

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Effects of blood components of preoperative autologous blood donation on functions of myelopoietic stem cells

Bai,

Su,

Wang

et al. 2023

Hematology

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Changes in CD34+ cell count in peripheral blood after whole blood donation

Cited by 3 publications

References 5 publications

Long-term Effects of Regular Whole Blood Donation on Peripheral Blood CD34+ Cells Population with using Leukoreduction Filters

Long-term Effects of Regular Whole Blood Donation on Peripheral Blood CD34+ Cells Population with using Leukoreduction Filters

Changes in hematological indices and lymphocyte subsets in response to whole blood donation in healthy male donors

Effects of blood components of preoperative autologous blood donation on functions of myelopoietic stem cells

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