Changes in carotenoid biosynthesis caused by variations of growth conditions in cultures of Rhodopseudomonas acidophila strain 7050

Heinemeyer, Ernst-August; Schmidt, Karin

doi:10.1007/bf00407761

Cited by 17 publications

(18 citation statements)

References 14 publications

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“…The carotenoid composition of this strain is rather independent of the light-intensity at which the cells are grown (Cogdell et al 1983). The antenna complexes of strain 7050 show a similar pattern of regulation with respect to light-intensity to strain 7750, however at low light-intensities their carotenoid composition is markedly altered and the cells go from a red-brown colour to a deep purple colour (Heinemeyer and Schmidt 1983, Cogdell et al 1983, Angerhofer et al 1986). …”

Section: Introductionmentioning

confidence: 86%

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The effect of growth conditions on the light-harvesting apparatus in Rhodopseudomonas acidophila

1993

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The detailed effect on the light-harvesting apparatus of three different wild-type strains of Rhodopseudomonas acidophila in response to changes in both light-intensity and temperature have been investigated. In all three strains at high light-intensities (160 μmol s m(2) and above) the only LH2 antenna complex synthesised is the B800-850 complex. In strains 7050 and 7750 as the light-intensity is lowered the B800-850 complex is gradually replaced by another type of LH2 the B800-820 complex. However, at no light-intensities studied is this changeover complete when the cells are grown at 30°C. If however, the light-intensity is lowered at temperatures below 25°C with strain 7750 there is a complete replacement of the B800-850 complex by the B800-820 complex. At all light-intensities and temperatures tested, strain 10050 only synthesised the B800-850 complex. Strain 7050 also responded to changes in light-intensity by altering its carotenoid composition. At high light-intensity the major carotenoids were rhodopin and rhodopin-glucoside, while at low light-intensities the major ones were rhodopinal and rhodopinal-glucoside. This change in carotenoid content started to occur at rather higher light-intensities than the switchover from B800-850 to B800-820.

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Section: Introductionmentioning

confidence: 86%

“…It is an example of a group of purple bacteria which is able to synthesise more than one type of variable (or LH2) light-harvesting complex depending on how the cells are grown (Thornber 1970, Hayashi et al 1982, Heinemeyer and Schmidt 1983, Cogdell et al 1983, Angerhofer et al 1986, Tadros and Waterkamp 1989.…”

Section: Introductionmentioning

confidence: 99%

The effect of growth conditions on the light-harvesting apparatus in Rhodopseudomonas acidophila

1993

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“…At HL, the major carotenoid in LH2 is rhodopin/rhodopin glucoside whereas at LL the major carotenoid is rhodopinal/ rhodopinal glucoside. 24,30 Interestingly, rhodopinal is about 50% more efficient as Fig. 4 Difference spectra of the NIR absorption from the Rhodopseudomonas acidophila 7050 membranes presented in Fig.…”

mentioning

confidence: 99%

“…Other factors such as the carbon source on which the cells are grown also affect the response to growth at different light intensities. 30 Rps. acidophila 7050 cells were grown under identical conditions at the same LL intensity but in one case using succinate as the carbon source and the other in the presence of pyruvate.…”

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confidence: 99%

Adaptation of Rhodopseudomonas acidophila strain 7050 to growth at different light intensities: what are the benefits to changing the type of LH2?

2018

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Typical purple bacterial photosynthetic units consist of light harvesting one/reaction centre 'core' complexes surrounded by light harvesting two complexes. Factors such as the number and size of photosynthetic units per cell, as well as the type of light harvesting two complex that is produced, are controlled by environmental factors. In this paper, the change in the type of LH2 present in the Rhodopsuedomonas acidophila strain 7050 is described when cells are grown at a range of different light intensities. This species contains multiple pucBA genes that encode the apoproteins that form light-harvesting complex two, and a more complex mixture of spectroscopic forms of this complex has been found than was previously thought to be the case. Femtosecond time resolved absorption has been used to investigate how the energy transfer properties in the membranes of high-light and low-light adapted cells change as the composition of the LH2 complexes varies.

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“…The detailed effects of growth conditions on the light-harvesting apparatus of Rps. acidophila has been reported in Gardiner et al (1993) and Heinemeyer and Schmidt (1983).…”

Section: Introductionmentioning

confidence: 99%

The purple photosynthetic bacterium Rhodopseudomonas acidophila contains multiple puc peripheral antenna complex (LH2) genes: Cloning and initial characterisation of four ?/? pairs

et al. 1996

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The genome of the purple non-sulphur photosynthetic bacterium Rhodopseudomonas acidophila has been found to contain multiple copies of puc light-harvesting (LH2) peripheral antenna complex genes. Three wild-type isolates each exhibiting dissimilar peripheral antenna complex phenotypes in response to growth at reduced light intensity, were found to contain different numbers of these genes. Twenty-three puc cross-hybridising clones were isolated from a genomic library constructed from Rhodopseudomonas acidophila strain 7050; two of which were examined further; 2.6kb from one clone was sequenced and found to contain three β/α gene pairs designated puc (1)BA, puc (2)BA and puc (3)BA. The putative translated polypeptides are very like, but not identical to those from B800-820 complexes and upstream sequence homologies suggests that this treble gene cluster has arisen through a relatively recent gene duplication event. From the other clone 0.6kb was sequenced and found to contain a further gene pair, puc (4)BA, which is capable of encoding apoproteins for a B800-850-like complex. When the cells are grown at 'high' or 'low' light intensity Northern analyses showed that only puc (4)BA is expressed under 'high' light conditions. Furthermore, pucBA mRNA transcripts were detected in all three species in the range 500-780 nt. In Rhodopseudomonas acidophila post-transcriptional regulatory mechanisms also play a role in determining the amount of peripheral antenna present in the intra-cytoplasmic membrane.

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Changes in carotenoid biosynthesis caused by variations of growth conditions in cultures of Rhodopseudomonas acidophila strain 7050

Cited by 17 publications

References 14 publications

The effect of growth conditions on the light-harvesting apparatus in Rhodopseudomonas acidophila

The effect of growth conditions on the light-harvesting apparatus in Rhodopseudomonas acidophila

Adaptation of Rhodopseudomonas acidophila strain 7050 to growth at different light intensities: what are the benefits to changing the type of LH2?

The purple photosynthetic bacterium Rhodopseudomonas acidophila contains multiple puc peripheral antenna complex (LH2) genes: Cloning and initial characterisation of four ?/? pairs

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