Changes in calmodulin and its mrna accompany reentry of quiescent (G0) cells into the cell cycle

Chafouleas, James G.; Lagacé, Lisette; Bolton, Wade E.; Boyd, A. E.; Means, Anthony R.

doi:10.1016/0092-8674(84)90075-8

Cited by 223 publications

(83 citation statements)

References 18 publications

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“…This is in agreement with observations from in vitro experiments, demonstrating new synthesis of calmodulin when quiescent cultured cells (CHO-Kl) re-enter in the cell cycle [27]. …”

Section: Calmodulin Mrna Measurementsupporting

confidence: 81%

Increased calmodulin synthesis in the pre‐replicative phase of rat liver regeneration

et al. 1988

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A cytosolic calmodulin wave has been described in the pre-replicative phase of rat liver regeneration.Here we demonstrate that this calmodulin increase is reduced by injection of cycloheximide (translation inhibitor) and actinomycin D (transcription inhibitor). In addition, we found that calmodulin mRNA levels are increased during the early pre-replicative period of liver regeneration, and this increase is affected by actinomycin D. Together, these results strongly indicate that the calmodulin wave observed in the pre-replicative phase of liver regeneration is due to de novo synthesis of this protein.

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“…This is in agreement with observations from in vitro experiments, demonstrating new synthesis of calmodulin when quiescent cultured cells (CHO-Kl) re-enter in the cell cycle [27]. …”

Section: Calmodulin Mrna Measurementsupporting

confidence: 81%

Increased calmodulin synthesis in the pre‐replicative phase of rat liver regeneration

et al. 1988

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“…Several studies have demonstrated that genes involved in DNA synthesis undergo transient expression during the cell cycle in mammalian cells and yeast (6,20,22,34,38). The levels of mRNA coding for calmodulin (11), and the transcription product of the tsl 1 gene which complements the tsl 1 mutation (18), a temperature-sensitive mutation in mammalian cells, were increased in the period from the GI to the early S phase. Expression of the mRNA that encodes p53 (33) and the mRNA that encodes PCNA (2), increased at the G,/S boundary.…”

Section: Discussionmentioning

confidence: 99%

Phase-Specific Polypeptides and Poly(A)⁺ RNAs during the Cell Cycle in Synchronous Cultures of Catharanthus roseus Cells

Kodama

Kawakami²,

Watanabe³

et al. 1989

Plant Physiol.

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This study shows an overall analysis of gene expression during the cell cycle in synchronous suspension cultures of Catharanthus roseus cells. First, the cellular cytoplasmic proteins were fractionated by two-dimensional gel electrophoresis and visualized by staining with silver. Seventeen polypeptides showed qualitative or quantitafive changes during the cell cycle. Second, the rates of synthesis of cytoplasmic proteins were also investigated by autoradiography by labeling cells with [S]methionine at each phase of the cell cycle. The rates of synthesis of 13 polypeptides were found to vary during the cell cycle. The silverstained electrophoretic pattem of proteins in the G2 phase in particular showed characteristic changes in levels of polypeptides, while the rates of synthesis of polypeptides synthesized during the G2 phase did not show such phase-specific changes.This result suggests that posttranslational processing of polypeptides occurs during or prior to the G2 phase. In the G1 and S phases and during cytokinesis, several other polypeptides were specifically synthesized. Finally, the variation of mRNAs was analyzed from the autoradiograms of In vitro translafton products of poly(A)+ RNA isolated at each phase. Three poly(A)+ RNAs increased in amount from the GI to the S phase and one poly (A)+ RNA increased preferentially from the G2 phase to cytokinesis.The cell cycle in eukaryotes consists of an ordered sequence of events, which include DNA synthesis, replication of organelles, and mitosis, and which are controlled by the ordered expression of genes. Many studies have been undertaken to examine the changes in protein patterns through which the sequential action of genes during the cell cycle is reflected, and to demonstrate that genes involved in cell proliferation are expressed transiently during the cell cycle. Two-dimensional gel electrophoresis has revealed the presence of several phase-specific proteins in mammalian cells which appear in the G2 phase (1) or which are synthesized in the S phase (10) and the G2/M phase (41). There are also reports (8,15,29) that no unique proteins are synthesized during the cell cycle in HeLa cells and yeast.

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“…One important role of growth factors is to induce or increase the levels of proteins which are essential for cell cycle progression (41). A number of genes with known products are cell cycle regulated, such as histones (40), thymidine kinase (27), calmodulin (9), and ornithine decarboxylase (19). Other genes whose expression is induced by growth factors or serum and may be cell cycle regulated have been identified (8,10,14,16,21,24,38).…”

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confidence: 99%

Isolation of the human gene that complements a temperature-sensitive cell cycle mutation in BHK cells.

Ittmann¹,

Greco²,

Basilico³

1987

Mol. Cell. Biol.

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We have cloned the human genomic DNA and the corresponding cDNA for the gene which complements the mutation of tsBN51, a temperature-sensitive (Ts) cell cycle mutant of BHK cells which is blocked in Gl at the nonpermissive temperature. After transfecting human DNA into tsBN51 cells and selecting for growth at 39.5°C, Ts+ transformants were identified by their content of human Alul repetitive DNA sequences. Following two additional rounds of transfection, a genomic library was constructed from a tertiary Ts' transformant and a recombinant phage containing the complementing gene isolated by screening for human AluI sequences. A genomic probe from this clone recognized a 2-kilobase mRNA in human and tertiary transformant cell lines, and this probe was used to isolate a biologically active cDNA from the Okayama-Berg cDNA expression library. Sequencing of this cDNA revealed a single open reading frame encoding a polypeptide of 395 amino acids. The deduced BN51 gene product has a high proportion of acidic and basic amino acids which are clustered in four hydrophilic domains spaced at 60-to 80-amino-acid intervals. These domains have strong sequence homology to each other. Thus, the tsBN51 protein consists of periodic repetitive clusters of acidic and basic amino acids.

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Changes in calmodulin and its mrna accompany reentry of quiescent (G0) cells into the cell cycle

Cited by 223 publications

References 18 publications

Increased calmodulin synthesis in the pre‐replicative phase of rat liver regeneration

Increased calmodulin synthesis in the pre‐replicative phase of rat liver regeneration

Phase-Specific Polypeptides and Poly(A)⁺ RNAs during the Cell Cycle in Synchronous Cultures of Catharanthus roseus Cells

Isolation of the human gene that complements a temperature-sensitive cell cycle mutation in BHK cells.

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Changes in calmodulin and its mrna accompany reentry of quiescent (G0) cells into the cell cycle

Cited by 223 publications

References 18 publications

Increased calmodulin synthesis in the pre‐replicative phase of rat liver regeneration

Increased calmodulin synthesis in the pre‐replicative phase of rat liver regeneration

Phase-Specific Polypeptides and Poly(A)+ RNAs during the Cell Cycle in Synchronous Cultures of Catharanthus roseus Cells

Isolation of the human gene that complements a temperature-sensitive cell cycle mutation in BHK cells.

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Phase-Specific Polypeptides and Poly(A)⁺ RNAs during the Cell Cycle in Synchronous Cultures of Catharanthus roseus Cells