Changes in bone microstructure and serum Tracp-5b level in male rats exposed to cadmium

Environmental level of cadmium exposure stimulates osteoclasts formation in male rats

“…Studies in vitro have indicated that low level of Cd could enhance osteoclast formation (Wilson et al, 1996;Chen et al, 2009aChen et al, , 2011Chen et al, , 2012. In this study, we observed that environmental level of Cd exposure could stimulate osteoclasts formation in male rats and RANKL/OPG may play important role.…”

“…It has been demonstrated that Cd could enhance the differentiation of osteoclasts from their progenitor cells and increased the activity of mature osteoclasts (Wilson et al, 1996). Our previous study also suggested that Cd could induce differentiation of RAW264.7 cells into osteoclasts in the presence of RANKL or osteoblasts (Chen et al, 2011(Chen et al, , 2012. Few studies show that Cd exposure could increase the number of TRAP positive osteoclasts in ovariectomized rats (Uriu et al, 2000).…”

“…Procedure of sample collection could see our previous studies (Chen et al, 2011). Briefly, at the 12th week, 24-h urine was obtained from all of rats using metabolic cages, and then all of rats were sacrificed by anesthesia and blood was collected from carotid artery.…”