Changes in blood lymphocyte populations in experimental bowel allograft rejection

Navarro-Zorraquino, Marta; Güemes, Antonio; Lozano, Ricardo; Larrad, Luis; Morandeira, M.J.; Salinas, José; Pastor, C.

doi:10.1007/978-3-662-00818-8_70

Cited by 1 publication

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“…The increase of CD8+25+Foxp3+ cell percentage was greater than the increase of CD4+25+Foxp3+ cell percentage. The previous studies [37][38][39] showed that earliness of rejection correlated with the percentage of CD8+ cells and the intensity of rejection with numbers of CD8+ cells; in addition, we observed a significant correlation between apoptosis and rejection, between CD8+ and CD54+ with apoptosis and with rejection, and between CD8+ and CD54+ 38 . Therefore, CD8+25+ cells are essential in rejection.…”

Section: Discussionsupporting

confidence: 75%

Implant of mesenchymal cells decreases acute cellular rejection in small bowel transplantation

Navarro-Zorraquino¹,

Pastor²,

Stringa³

et al. 2020

CIRU

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Objective: The objective of the study was to show adipose tissue-derived mesenchymal stem cells (AD-MSCs) immunomodulatory effects in small bowel transplantation (SBTx). Materials and methods: Forty Wistar Han rats (age: 10-12 weeks): were allogenic receptor rats and were allotted in 2 groups. Control group: rats undergoing orthopic SBTx ; AD-MSCs group: rats undergoing orthotopic SBTx plus AD-MSCs. Male Lewis rats were allogeneic small bowel donors. Rejection was confirmed by histological study of the explanted intestine, enterocyte apoptosis was determined in crypts and the lamina propria of the small bowel. Cytokine concentration levels (enzyme-linked immunosorbent assay) (interleukin , tumor necrosis factor-alpha, and transforming growth factor [TGF]-b1) and cell percentages (flow cytometry) (CD3+ CD4+, CD8+, CD4+/25+, CD8+/25+, CD4+/25+/Foxp3+, and CD8+/25+/Foxp3+) were assessed in peripheral blood preoperatively and after death. Results: Treatment with AD-MSCs produced a significantly lower risk of rejection in the first 7 postoperative days (five rejection cases among 20 rats in the control group and only one case in the AD-MSCs group). T reg cells and TGFb1 levels showed a significant increase in the AD-MSCs group. Conclusions: The local implantation of AD-MSC in the anastomosis and the intestinal lumen can induce a regulatory immune response, by increasing the percentages of Treg cells and TGb-1 levels, leading to a lower risk of acute rejection by cell mediation, in the first 7 days of the intestinal transplant. We think that the implantation of AD-MSCs, in the anastomoses and in the lumen of the donor intestine, could give rise to a chimera of donor-recipient cells.

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Section: Discussionsupporting

confidence: 75%