Changes in Blood Components in Aphtha Patients with Excess Heat

Lu, Qin; Li, Yan; Jiao, Yifeng; Fu, Danqing; Li, Ye; Ji, Jinjun; Xie, Guanqun; Fan, Yongsheng; Xu, Li

doi:10.1155/2016/7219257

Cited by 4 publications

(3 citation statements)

References 15 publications

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“…Main symptoms of heat syndrome are manifested on the head and face, including aphtha, oral ulcer, glossitis, and swollen gums. A few researchers have explored the major manifestations of TCM heat syndrome, such as oral ulcer [6], but the biological essence of heat syndrome is not completely understood yet. In the study, AZC herbs were used to set up heat syndrome rat model [5, 7], and we observed manifestation of heat syndrome.…”

Section: Discussionmentioning

confidence: 99%

The Pungent and Hot Chinese Herbs Cause Heat Syndrome in Rats by Affecting the Regulatory T Cells

Xie

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Heat syndrome is a folk saying in China, which is used to describe people with symptoms such as aphtha, oral ulcer, glossitis, swelling and aching of gingiva, and dry eye. Aconitum carmichaelii Debx. (A), Zingiber officinale Rosc. (Z), and Cinnamomum cassia Presl (C) are the representatives of pungent and hot Chinese herbs which may cause heat syndrome. In order to explore the mechanism of pungent herbs-induced heat syndrome, rats were treated with AZC extracts at different concentrations and at different time periods. A series of cytokines were determined using the cytokine antibody array; some immunosuppressive cytokines, including TGF-β, IL-10, and IL-35, significantly increased in AZC group as compared with control group. Higher mRNA expressions of Foxp3, TGF-β, IL-10, and IL-35 were found in the spleen and thymus of rats after treatment for 18 days based on RT-PCR. Flow cytometry result revealed that the percentage of CD4+CD25+ Treg cells and Foxp3+CD4+CD25+ Treg cells in spleen lymphocytes showed an increasing trend from the 3rd day to the 18th day after treatment with middle dose of AZC extracts. It is speculated that extracts of AZC herbs may affect the development of heat syndrome by influencing Treg cells and immunosuppressive cytokines.

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Section: Discussionmentioning

confidence: 99%

The Pungent and Hot Chinese Herbs Cause Heat Syndrome in Rats by Affecting the Regulatory T Cells

Xie

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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“…Aphtha is a common and frequently occurring disease related to the immune system; it can be divided into excess heat and Yin deficiency in TCM. Qin et al [53] found abnormal immune regulation in aphtha patients induced by excess heat, such as abnormal serum protein expression (interleukin-4 (IL-4), matrix metalloproteinase (MMP)-19, MMP-9, and activin A) and a higher percentage of CD4+ CD25+ regulatory T cells (Treg cells) in the peripheral blood lymphocytes, with obvious swelling and pain, red tongue, and yellow coating.…”

Section: Close Correlation Between Tongue Coating and Diseasementioning

confidence: 99%

Biology of the Tongue Coating and Its Value in Disease Diagnosis

et al. 2017

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Tongue diagnosis is one of the most important diagnostic tools in traditional Chinese medicine and has been verified for thousands of years. However, its subjectivity and repeatability has been disputed continuously. The tongue coating as the primary coverage of tongue diagnosis provides more objectivity and reproducibility due to its relatively clear molecular basis; it also has a close relationship with many system diseases and may be used as a potentially valuable disease diagnostic tool. This article describes the material basis of the tongue coating, including its biology (epithelial cells, blood cells, vascular endothelial cells, and bacteria) and its metabolites; moreover, we summarize the diseases that are most correlated with the tongue coating. This will be valuable not only for fundamental research of tongue diagnosis but also for the diagnosis and differential diagnosis of disease. We suppose that the tongue coating could serve as a valuable auxiliary diagnosis tool in many diseases, and more research should focus on how to colligate the various information about the tongue and provide useful information for disease diagnosis.

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“…This syndrome classi cation enables individualized treatment clinically. Herbs for excess-heat syndrome of RAS mainly involves fructus forsythia, folium isatidis, radix scutellariae, rhizoma coptidis, and for yinde ciency syndrome mainly involves herba asari, radix rehmanniae, cortex phellodendri, radix ophiopogonis, cortex cinnamomi, and other herbs [8]. The treatment in RAS based on syndrome differentiation has won the international acceptance, but the molecular mechanism of difference syndrome still remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Identification and Functional Analysis of Serum Specific MiRNAs in Recurrent Aphthous Somatitis Patients With Excess-heat or Yin-deficiency

Bao

Zhu

Zhang

et al. 2020

Preprint

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Background:To identity key miRNAs as signatures for recurrent aphthous stomatitis(RAS)with Excess-heat or Yin-deficiency bymiRNA microarrays. Method: Serum samples were collected from patients meeting the RAS diagnostic criteria of excess-heat oryin-deficiencysyndrome and healthy individuals. CoremicroRNAs (miRNAs) were then identified under miRNA microarray analyses. Target prediction and bioinformatic analyses were carried out andgene-pathway-networks werevisualized to better understand the relationship between differentgenes and pathways.Result:(1) 90 individuals meeting the inclusion criteria were collected in this study, of which 30 were normal control, 30 were patients of excess-heat syndrome and the rest were patients ofyin-deficiency syndrome. Among them, 9 miRNAs werescreened out in excess-heat syndrome group, with 1 upregulated and 8 downregulated. And four randommiRNAs(hsa-miR-20b-5p, hsa-miR-122-5p, hsa-miR-483-5p and hsa-miR-3197) were validatedby real-time PCR method. 14 miRNAs werescreened out in yin-deficiency syndrome group(7 upregulated and 7 downregulated). And hsa-miR-17-5p, hsa-miR-106-5p and hsa-miR-20b-5p were validated. (2)A total of 4776 target genes were identified for the validated 9 miRNAs in excess-heat syndrome group.These targets were enriched inGO categories including nervous system development, homophilic cell adhesion via plasma membrane adhesion molecules, and calcium ion binding and KEGG pathway such as proteoglycans in cancer, P13K-AKT signaling pathway and Calcium signaling pathway. 10172 target genes were identified for the validated 14 miRNAs in yin-deficiency syndrome group. The enrichedGO categories included protein binding, positive regulation of transcription from RNA polymerase II promoter and membrane andenrichedKEGG pathway included pathways in cancer, MAPK signaling pathway and Ras signaling pathway.Conclusion:Hsa-miR-20b-5p in patients with RAS could act as the novel target for syndromeclassification of the disease. It is upregulated in RAS patients with excess-heat syndrome while downregulated in patients with yin-deficiency syndrome. The PI3K-Akt signaling pathway and MAPK signaling pathway and related target genes may provide new insights into the molecular mechanisms of RAS with excess-heat syndrome or yin-deficiency syndrome, respectively.

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Changes in Blood Components in Aphtha Patients with Excess Heat

Cited by 4 publications

References 15 publications

The Pungent and Hot Chinese Herbs Cause Heat Syndrome in Rats by Affecting the Regulatory T Cells

The Pungent and Hot Chinese Herbs Cause Heat Syndrome in Rats by Affecting the Regulatory T Cells

Biology of the Tongue Coating and Its Value in Disease Diagnosis

Identification and Functional Analysis of Serum Specific MiRNAs in Recurrent Aphthous Somatitis Patients With Excess-heat or Yin-deficiency

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