Changes in blood chemistry, hematology, and histology caused by a selenium/vitamin E deficiency and recovery in chicks

Abstract

Exudative diathesis, a condition caused by a selenium (Se)/vitamin E deficiency, was studied in chicks. Trios of chicks that showed clinical signs of exudative diathesis were matched for severity. One was injected subcutaneously with 0.5 mL distilled water, and the other two received 15 microg of Se in 0.5 mL distilled water. A chick fed a diet with supplemental Se also received 0.5 mL distilled water. Blood was collected from three chicks 2 d after injection, and from the other chick, 6 d after injection. After blood was collected, pectoral muscle and bone marrow were collected. Deficient chicks showed varying degrees of necrosis in pectoral muscle, whereas recovering chicks had extensive fibrosis in pectoral muscle. An analysis of blood showed differences in CO2, glucose, Se, glutathione peroxidase, alanine aminotransferase, aspartate aminotransferase, and creatine kinase. Heterophils and monocytes were increased in deficient chicks; lymphocytes, basophils, and hemoglobin decreased. After 6 d of recovery, all of the changes noted above were correcting toward normal. Eosinophils, in contrast, were unaffected by a deficiency, but increased in recovering chicks. It is hypothesized that cytokines associated with the inflammatory response accentuate the clinical signs of exudative diathesis.

Mechanism of radioprotection by δ-tocotrienol: pharmacokinetics, pharmacodynamics and modulation of signalling pathways

“…Previous studies have shown that treatment with vitamin E increased the number of colony forming units of erythroid precursors (CFU-E), enhanced erythropoiesis and haemoglobin levels, and corrected experimentally induced anaemia in laboratory animals [20][21][22]. Our data indicate that the stimulatory effect of DT3 is not limited to erythropoiesis, but extends to leukopoiesis and megakaryopoiesis.…”

“…During irradiation, the animals were placed in well-ventilated Plexiglas chambers made specifically for mouse irradiation. The mid-line dose to the animals was delivered at a dose rate of 0.6 Gy min 21 . An alanine/electron spin resonance (ESR) dosimetry system (American Society for Testing and Material Standard E 1607) was used to measure dose rates (to water) in the cores of acrylic mouse phantoms.…”

“…An aliquot of 50 ml was injected into the high-performance liquid chromatography (HPLC) system (equipped with a 20A-T solvent pump, autosampler, and in-line 20AD UV and 10Rxl fluorescent detectors; Prominence, Shimadzu, Columbia, MD). The pump was run at a flow rate of 0.8 ml min 21 and the mobile phase was composed of acetonitrile:water (90:10). The total run time was 9 min and chromatographic separation was carried out by a Kinetex C18 reverse phase column (2.6 mm, 4.6 mm610 cm; Phenomenex, Terrance, CA).…”

“…The calibration curve for DT3 was found to be linear in the range of 1-300 mM (r 2 50.999) (Figure 1a). The plasma concentration-time curve for DT3 following a single sc administration of 300 mg kg 21 to CD2F1 mice is depicted in Figure 1b. The peak plasma concentration (Cmax) of DT3 was 194¡25.4 mM l…”

Synthesis and Characterization of Selenium-sorbed Ca2+-exchanged Zeolite A for High-performance Feed

“…동물이나 사람은 셀 레늄 결핍으로 영양성 질병이 유발되는데, 생리적으로 성장 지연, 피부병, 탈모, 시각장애, 번식장애, 간이나 근육의 괴 사가 일어나며, 산란계에서는 셀레늄 결핍이 산란율과 부화 율을 떨어뜨린다 (Cator and Scott, 1974). 셀레늄 결핍은 혈액 조성에도 영향을 주어 닭에서 미성숙 혈구의 증가를 야기하며 (Bartholomew et al, 1998), 닭에 셀레늄과 비타 민 E가 결핍된 사료를 급여할 경우 심출성 소질 (Nouguchi et al, 1973;Bartholomew et al, 1998), 뇌연화증 (Century and Hurwitt, 1964;Combs and Hady, 1991), 췌장위축 (Thompson and Scott, 1969;Cantor et al, 1975 (Fig. 3).…”