Changes in Auxin and Abscisic Acid-Like Activities in Grape Berries

Niimi, Yoshiyuki; Ohkawa, Makio; Torikata, Hirotaka

doi:10.2503/jjshs.46.139

Cited by 9 publications

(11 citation statements)

References 21 publications

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“…This stimulation of mitochondrial 02 uptake by ABA (Figs. 1 and 2) coincides with the observation by Niimi et al (9) and Niimi and Torikata (10) that in the ripening process of grape berries, the respiratory rate and ABA content in the berries increased in parallel. Senescence of many organs or tissues in plants is related to ABA level (4), and the increase of ABA is known to induce changes in the cellular level of organic acids and sugars (1 1).…”

Section: Resultssupporting

confidence: 74%

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Activation of O₂ Uptake and NAD-Specific Isocitrate Dehydrogenase in Mitochondria Isolated from Cotyledons of Castor Bean by cis, trans-Abscisic Acid

Tezuka

Yamamoto²,

Kondo³

1990

Plant Physiol.

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The rate of 02 uptake and the activity of NAD-specific isocitrate dehydrogenase (NAD-ICDH) of mitochondria isolated from castor bean cotyledons were increased by added cis,trans-abscisic acid (ABA) in an in vitro system, while the NADP-specific isocitrate dehydrogenase (NADP-ICDH) was not affected by cis,trans-ABA. Trans,trans-ABA showed only a slightly inhibitory effect on 02 uptake. The Vmax value for the isotherm of isocitrate by the enzyme was also increased by cis,trans-ABA. The isocitrate Km value for the enzyme with cis,trans-ABA was calculated to be approximately 249.8 micromolar, while the S05 for the enzyme without the ABA was 151.6 micromolar. The n value calculated from the slopes of Hill plots of the reaction velocity of NAD-ICDH against isocitrate concentration was 1.5 in the mitochondrial fraction in the absence of ABA, and cis,trans-ABA treatment decreased the value to 1.0. Cis,trans-ABA also partly overcame the inhibition of NAD-ICDH activity by ATP. It has been reported that the respiratory rate (10) and ABA content (9) in grape, expressed on a per berry basis, increased during the ripening process after veraison (which means the onset of maturation [13]). These phenomena suggest a close relationship between ABA content and respiratory metabolism in mature tissues and organs of plants.Goldthwaite (5) observed that ABA stimulates oxygen consumption during senescence of Rumex leaf discs. Hourmant and Penot (6) reported that the rate of respiration of aerated potato tuber discs increased after a 24-h aging period with ABA in the dark. Tillberg et al. (16) found that respiration in Lemna gibba L. was significantly stimulated 2 to 7 d after ABA treatment. In the unicellular green algae, Chlorellafusca and Ankistrodesmus brauni, dark respiration was increased by ABA (17).In the present work, we studied the stimulating action of ABA on respiration of mitochondria isolated from cotyledons of castor beans and found evidence for the regulation of enzyme activity by ABA in an in vitro system. MATERIALS AND METHODS Plant MaterialsCastor beans (Ricinus communis L.) were grown under the conditions described previously by Kondo and Sugahara (7).The plants were grown for 2 to 3 weeks at 25 ± 0.5°C during the day and 20 ± 0.5°C at night with 70 ± 5% RH in an environmentally controlled glasshouse under natural light conditions. The growth substratum was composed of vermiculite: peat moss:perlite:fine gravel (2:2:1:1, v/v/v/v). A nutrient solution containing 8 g of magnesia lime and 4 g of hyponex per liter was supplied twice a week to each 1.9 L pot. Isolation of MitochondriaFive pairs of castor bean cotyledons were cut into small pieces (about 15 g) and homogenized with 45 mL of 25 mM Hepes buffer (pH 7.8) containing 0.35 M mannitol, 0.25 M sucrose, and 0.1 mg Na-2-mercaptobenzothiazole and 1 mg BSA per mL (14) using a Homo blender (18,000 rpm, 100 W, Sakuma Seisakusho, Ltd., Tokyo) at a quarter of maximum speed for 20 s. The homogenate was squeezed through four layers of gauze and centrifuged for 10...

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Section: Resultssupporting

confidence: 74%

“…It has been reported that the respiratory rate (10) and ABA content (9) in grape, expressed on a per berry basis, increased during the ripening process after veraison (which means the onset of maturation [13]). These phenomena suggest a close relationship between ABA content and respiratory metabolism in mature tissues and organs of plants.…”

mentioning

confidence: 99%

Activation of O₂ Uptake and NAD-Specific Isocitrate Dehydrogenase in Mitochondria Isolated from Cotyledons of Castor Bean by cis, trans-Abscisic Acid

Tezuka

Yamamoto²,

Kondo³

1990

Plant Physiol.

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“…Many attempts have been made to describe and explain the mechanisms that control the changes in fruit growth rate, in clud ing hypotheses that developmental changes in the pericarp are linked to changes in seeds or result from competition with vegetative growth (Matsui et al, 1985;Niimi and Torikata, 1979;Novello et al, 1999;Staudt et al, 1986). Several plant hormones may play an important role in the growth and development of grape berries (Brenner et al, 1989;Cawthon and Morris, 1982;Inaba et al, 1976;Niimi et al, 1977;Pandey and Singh, 1989;Tromp, 1989). Much attention and were trained on a double vertical trellis with a fan system widely used in commercial vineyards in China.…”

Section: Methodsmentioning

confidence: 99%

Growth and Developmental Responses of Seeded and Seedless Grape Berries to Shoot Girdling

Zhang¹,

Luo²,

Wang³

et al. 2003

jashs

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The relationship of assimilate supply to grape (Vitis vinifera L.) berry growth and development was studied with a seeded (`Kyoho') and a seedless (`Seedless Wuhehong') cultivar. A single shoot girdling between the second and third nodes below the basal cluster at the end of Stage I of berry growth shortened Stage II (the lag phase) of `Kyoho' grape berries by 10 days, and eliminated Stage II in `Seedless Wuhehong' grape berries. Double shoot girdling between the second and third nodes below the basal cluster and above the upper cluster, respectively, at the same time at the end of Stage I, advanced Stage II by 3 days in both cultivars. Normal accumulation of dry weight in the `Kyoho' grape berry is in a double sigmoidal pattern, but it became a single sigmoidal pattern in response to a single basal girdling. The highest percent moisture in berries was at 20 days after full bloom. Rapid changes in berry pectin substances lagged behind those of soluble solids and titratable acidity, and behind the onset of berry softening at veraison in `Kyoho', but not in `Seedless Wuhehong', for which the three processes were concurrent. It is suggested that the slow growth of the berries during Stage II is a result of a decrease in the rate of water accumulation on a whole berry basis and a decrease in accumulation of dry matter in the skin and flesh (pericarp) due to assimilate competition within grapevines and within berries. The relationships between levels of endogenous hormones (IAA, GA3, zeatin, zeatin riboside, and ABA) and berry growth were also studied with `Kyoho' grapes. The results showed that the slow growth of grape berries during Stage II was associated with assimilate competition between the skin-flesh (pericarp) and seeds, and with peak shifts of concentrations of IAA, GA3, zeatin and zeatin riboside. Changes in ABA levels were closely associated with ripening and senescence during late Stage III.

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“…Auxin and ABA-like activities in the extracts were determined by the slightly modified Avena coleoptile straight growth test (24,25). Activities of cytokinin-like substance (14) and gibberellin-like substance (4) were determined by the radish cotyledon assay and the barley endosperm assay, respectively.…”

Section: Bioassay Of Endogenous Hormonesmentioning

confidence: 99%

Changes in Endogenous Plant Hormones in the Xylem Sap of Grapevines during Development

Niimi

Torikata

1978

Engei Gakkai zasshi

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Changes in Auxin and Abscisic Acid-Like Activities in Grape Berries

Cited by 9 publications

References 21 publications

Activation of O₂ Uptake and NAD-Specific Isocitrate Dehydrogenase in Mitochondria Isolated from Cotyledons of Castor Bean by cis, trans-Abscisic Acid

Activation of O₂ Uptake and NAD-Specific Isocitrate Dehydrogenase in Mitochondria Isolated from Cotyledons of Castor Bean by cis, trans-Abscisic Acid

Growth and Developmental Responses of Seeded and Seedless Grape Berries to Shoot Girdling

Changes in Endogenous Plant Hormones in the Xylem Sap of Grapevines during Development

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Changes in Auxin and Abscisic Acid-Like Activities in Grape Berries

Cited by 9 publications

References 21 publications

Activation of O2 Uptake and NAD-Specific Isocitrate Dehydrogenase in Mitochondria Isolated from Cotyledons of Castor Bean by cis, trans-Abscisic Acid

Activation of O2 Uptake and NAD-Specific Isocitrate Dehydrogenase in Mitochondria Isolated from Cotyledons of Castor Bean by cis, trans-Abscisic Acid

Growth and Developmental Responses of Seeded and Seedless Grape Berries to Shoot Girdling

Changes in Endogenous Plant Hormones in the Xylem Sap of Grapevines during Development

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Activation of O₂ Uptake and NAD-Specific Isocitrate Dehydrogenase in Mitochondria Isolated from Cotyledons of Castor Bean by cis, trans-Abscisic Acid

Activation of O₂ Uptake and NAD-Specific Isocitrate Dehydrogenase in Mitochondria Isolated from Cotyledons of Castor Bean by cis, trans-Abscisic Acid