Changes in ATP level and iron-induced ultra-weak photon emission in bull spermatozoa, caused by membrane peroxidation during thermal stress.

Gumińska, M; Kedryna, T; Laszczka, A.; Godlewski, M.; Sławiński, Janusz; Szczęśniak-Fabiańczyk, B.; Kwiecinska, T.; Rajfur, Zenon; Wierzuchowska, D.

doi:10.18388/abp.1997_4450

Cited by 7 publications

(3 citation statements)

References 20 publications

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“…Measurement of ATP level may provide information on the efficiency of metabolic mechanisms that control energy production and consumption in the cell. However, data concerning the relationship between ATP level, sperm motility and fertility are inconsistent (GUMIÑSKA et al 1997;MINELLI et al 1999;TORRES-FLORES et al 2011) and this issue needs further study.…”

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confidence: 97%

Motility Parameters and Intracellular ATP Content of Rabbit Spermatozoa Stored for 3 Days at 15°C

Gogol¹

2013

folia biol (krakow)

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The effect of semen storage duration on motility parameters and ATP content of rabbit spermatozoa were investigated. Ejaculates were collected from 9 New Zealand White male rabbits and diluted with a commercial rabbit semen extender Galap. Semen was stored at 15 degrees C for 3 days. On each day of storage sperm motility and intracellular ATP content were evaluated. Sperm motility parameters were assessed using the computer-assisted sperm analysis (CASA) system and ATP content using the bioluminescence method. The time of storage had a significant effect on sperm motility parameters (except straight-line velocity) and ATP content. A significant correlation was observed between motility parameters and sperm ATP content. The motility parameters most strongly correlated with ATP content were total motile spermatozoa (r = 0.6364), progressively motile spermatozoa (r = 0.529), amplitude of lateral head displacement (r = 0.4178), curvilinear velocity (r = 0.4111) and average path velocity (r = 0.3743). Results show that motility parameters determined using the CASA system and intracellular ATP content are sensitive indicators of sperm quality during in vitro storage and may be useful for estimation of in vivo fertilizing ability of rabbit semen.

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confidence: 97%

Motility Parameters and Intracellular ATP Content of Rabbit Spermatozoa Stored for 3 Days at 15°C

Gogol¹

2013

folia biol (krakow)

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“…However, abnormal sperm midpiece organization may be unrelated to mitochondrial energy efficiency. DFX may help to remove iron overload from sperm mitochondria, which has been associated with low ATP generation and alteration of sperm motility as well as with increased ROS generation [ 4 , 54 – 58 ]. It is of interest to note that in agreement with our observations, Perera et al reported that an increase in sperm DNA damage was recorded upon initiation of iron chelation therapy in β -thalassemic patients with iron overload [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

Deferasirox, an Iron‐Chelating Agent, Improves Testicular Morphometric and Sperm Functional Parameters in a Rat Model of Varicocele

Rahmani

Tavalaee

Hosseini

et al. 2021

Oxidative Medicine and Cellular Longevity

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Varicocele is characterized by testicular dysfunction that originates from hyperthermia and hypoxia, leading to defects in testicular tissue and altered spermatozoa structure and function. The varicocele testis is characterized by the presence of intracellular iron deposits that contribute to the associated oxidative stress. Therefore, we tested the hypothesis that administration of an iron-chelating agent, such as deferasirox (DFX), could potentially mitigate the consequences of varicocele on testicular tissue and spermatozoa. Using a well-established rat model of varicocele (VCL), we show that treatment with DFX partially improved the structure and function of the testis and spermatozoa. In particular, sperm motility was markedly restored whereas abnormal sperm morphology was only partially improved. No significant improvement in sperm count was observed that could be associated with the proapoptotic response observed following iron chelation treatment. No reduction in oxidative damage to spermatozoa was observed since lipid peroxidation and DNA integrity were not modified. This was suggested to be a result of increased oxidative stress. Finally, we also saw no indication of attenuation of the endoplasmic reticulum/unfolded protein (ER/UPR) stress response that we recently found associated with the VCL testis in rats.

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“…The chemical energy required for sperm motility is supplied by the mitochondria in the form of adenosine triphosphate (ATP). The firefly luciferin-luciferase assay has been used to quantity ATP content in sperm from a wide variety of species, including boar (Aalbers et al, 1985), bull (Guminska et al, 1997) and human (Mendeluk et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Comparison of Motility, Acrosome, Viability and ATP of Boar Sperm with or without Cold Shock Resistance in Liquid Semen at 17째C and 4째C, and Frozen-thawed Semen

Yi¹,

Li²,

Kim³

et al. 2008

Asian Australas. J. Anim. Sci

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This study was designed to analyze boar sperm to compare motility, acrosome morphology, viability and ATP by various preservation methods between Duroc boar A with cold shock resistance sperm and Duroc boar B with cold shock sensitivity sperm. Semen volume, sperm concentration, motility and normal acrosome between Duroc boar A and B did not show any differences within 2 h after collection. There were no differences in sperm motility and normal acrosome between boar A and B at 1 day of preservation at 17°C and 4°C, respectively. However, sperm motility and normal acrosome from 2 day of preservation at 17°C and 4°C, respectively, were higher for boar A than boar B. The frozen-thawed sperm motility and normal acrosome were higher for boar A than boar B. The sperm viability and ATP concentration according to storage period of liquid semen at 17°C and 4°C were higher for boar A than boar B. Also, the sperm viability and ATP concentration of frozen-thawed semen were higher for boar A than boar B. In conclusion, we found out that the original quality of boar semen with cold shock resistance sperm played an important role.

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Changes in ATP level and iron-induced ultra-weak photon emission in bull spermatozoa, caused by membrane peroxidation during thermal stress.

Cited by 7 publications

References 20 publications

Motility Parameters and Intracellular ATP Content of Rabbit Spermatozoa Stored for 3 Days at 15°C

Motility Parameters and Intracellular ATP Content of Rabbit Spermatozoa Stored for 3 Days at 15°C

Deferasirox, an Iron‐Chelating Agent, Improves Testicular Morphometric and Sperm Functional Parameters in a Rat Model of Varicocele

Comparison of Motility, Acrosome, Viability and ATP of Boar Sperm with or without Cold Shock Resistance in Liquid Semen at 17째C and 4째C, and Frozen-thawed Semen

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