Changes in antioxidant levels in Oryza sativa L. roots subjected to NaCl-salinity stress

Khan, Mohammad Hussain; Singha, Lal Bihari; Panda, Sanjib Kumar

doi:10.1007/s11738-002-0004-x

Cited by 89 publications

(66 citation statements)

References 22 publications

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“…Such observation was described in leaves of tomato [49,50], sugar [51], sesame [52], plantain [53] and rice [54] and in the roots of rice [55]. Increase in H 2 O 2 and TBARS in response to salt stress was observed in wheat [56], rice [57] and tomato [58,59]. Regarding these results we conclude that lupine embryo axes experienced oxidative stress and a transient increase in TBARS was a consequence of salt shock occurring in first hours of NaCl treatment.…”

Section: Discussionsupporting

confidence: 69%

Ultrastructural and antioxidative changes in lupine embryo axes in response to salt stress

2013

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Embryo axes of lupine (Lupinus luteus L. 'Mister') were subjected to 0.1 M NaCl salt stress for 24 and 48 h. The ultrastructure modification and adjustment of antioxidant enzymes activities and izoenzymes profiles were observed. In cells of lupine embryo axes grown for 48 hours in medium with 0.1 M NaCl mitochondria took the forked shape and bulges of the outer mitochondrial membranes appeared. Moreover, the inflating and swelling of rough endoplasmic reticulum (RER) lumen and fragmentation of RER were noticed. The level of H 2 O 2 was higher in salt treated embryo axes after 24 hours and increase of thiobarbituric acid reactive substances was observed after both 24 and 48 h of salt treatment. Native gel electrophoresis showed increased intensities of bands for catalase isozymes in response to salt stress, whereas activity of catalase was higher only in embryo axes grown for 48 h in control conditions. Appearance of two new isoforms of ascorbate peroxidase was observed after 48 h only under control condition, however increased activities were stated for both control and salt-stress condition after 48 h. No changes in isozymes pattern for superoxide dismutase were observed, but significant decrease in superoxide dismutase activity was noticed in relation to time and salt stress. Possible role of these enzymes in salt stress tolerance is discussed. The 0.1 M salt stress is regarded as a middle stress for lupine embryo axes and the efficiency of stress prevention mechanisms is proposed. Keywords: salt stress, lupine, ultrastructure, antioxidantJournal homepage: pbsociety.org.pl/journals/index.php/asbp ORIGINAL RESEARCH PAPER Received: 2013.04.18 Accepted: 2013.09.26 Published electronically: 2013.11.12 Acta Soc Bot Pol 82(4):303-311 DOI: 10.5586/asbp.2013 Ultrastructural and antioxidative changes in lupine embryo axes in response to salt stress Łukasz Wojtyla*, Magda Grabsztunowicz, Małgorzata Garnczarska

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Section: Discussionsupporting

confidence: 69%

Ultrastructural and antioxidative changes in lupine embryo axes in response to salt stress

2013

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“…Proline accumulation may also help in nonenzymic free radical detoxifications [18; 7, 13]. Increasing proline level is considered to help the cells in osmoprotection as well as in regulating the redox potential, scavenging hydroxyl radicals and gives the protection against denaturation of various macromolecules [13]. In the present context, a uniform increase in the proline level was noticed in 9 and 11 days old rice seedlings subjected to Cr +6 (100 µM) treatment.…”

Section: Results and Discussion Growth Parameter Studymentioning

confidence: 71%

“…Bioinfo Publications Proline accumulation is an important parameter to recognize the stress impact on plants [14,24]. For example, stress induced proline biosynthesis was observed in many plants in response to salt stress [13,16]. Proline accumulation may also help in nonenzymic free radical detoxifications [18; 7, 13].…”

Section: Results and Discussion Growth Parameter Studymentioning

confidence: 99%

Effect of Cr+6 and Chelating Agents on Growth, Pigment Status, Proline Content and Chromium Bioavailability in Rice Seedlings

Mohanty¹,

Hk²

2011

Int J of Biot Appl

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Abstract-Hexavalent Chromium (Cr +6 ) induced several toxicological changes in the developing rice (Oryza sativa L.) seedlings as showed from present in vitro hydroponic study. Growth parameters, physiological and biochemical alterations in rice were studied in response to the application of toxic concentration of Cr +6 with addition of chelating agents. Rice seedlings growing in nutrient solutions supplemented with Cr +6 (100µM) showed noticeable decrease in root and shoot length, fresh and dry matter; whereas addition of chelating agents like EDTA, DTPA and EDDHA with Cr +6 at 10 µM stimulates the growth. Chlorophyll and carotenoid biosynthesis was also declined with the treatment of Cr +6 (100µM), but application of Cr +6 -EDTA (10µM) amplified the pigment biosynthesis. Rice seedlings treated with Cr +6 (100µM) showed increased proline content and high Cr accumulation in root whereas treatments of Cr +6 -DTPA (10µM) enhanced the shoot Cr bioaccumulation as compared to other treatments. Supplementation of Cr +6 -EDTA (10µM) showed increased phytoextraction of Cr in roots of rice seedlings. Transportation index (Ti) of seedlings grown in Cr +6 (10µM) and Cr +6 -DTPA (10µM) was found more in comparison to others. The variations in the total accumulation rate (TAR) of Cr of different seedlings were negligible.

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“…In addition to having a role as an osmolyte, proline is able to increase stability and solidity of the membrane in different situations as an enzymatic protector. Also, accumulation of proline has a non-enzymatic role in detoxification of free radicals (Khan et al, 2002). It seems increasing in proline concentration is not the reason for salt 4.531** 3.174** 3.171** T ** And ns represent significant at 1% and non-significant, respectively Fig.…”

Section: Protein Identificationmentioning

confidence: 94%

Canola Seedling Response to NaCl Stress – a Proteomic Approach

Alagoz

Toorchi

Bandehagh

2016

Not Bot Horti Agrobo

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Salinity is a major abiotic stress worldwide, which causes a tremendous loss in the quality and quantity of agricultural products. In order to identify defense mechanisms to salinity-induced stress, a two-dimensional electrophoresis approach was used to investigate proteins achieved from canola cultivar 'Option 500' seedlings, after plants had been treated with NaCl. The 300 mmol NaCl treatment caused a significant decrease in fresh and dry weight as well as plant height and increment of proline content compared with control group. There were no significant changes in the quality of catalase and peroxidase activity. Out of 110 repeatable protein spots, 44 were registering change, according to induction factor index. 7 spots were recognized significant at 1% probability level statistically. These proteins were involved in energy production, ROS scavenging and suppressor, and those involved in photosynthesis. Production of chloroplastic fructose-bisphosphate aldolase enzyme along with carbohydrate metabolisms of Kelvin cycle and glycolysis increases under salinity condition. The amount of Cu/Zn SOD in chloroplast reduces in response to salinity which results from an increase in OH -radicals. Expression of photosynthesis proteins have been reduced probably as a consequence of the drop in osmotic pressure resulted from water scarcity and stomata closure, which reduces the availability of Co2.

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Changes in antioxidant levels in Oryza sativa L. roots subjected to NaCl-salinity stress

Cited by 89 publications

References 22 publications

Ultrastructural and antioxidative changes in lupine embryo axes in response to salt stress

Ultrastructural and antioxidative changes in lupine embryo axes in response to salt stress

Effect of Cr+6 and Chelating Agents on Growth, Pigment Status, Proline Content and Chromium Bioavailability in Rice Seedlings

Canola Seedling Response to NaCl Stress – a Proteomic Approach

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