Changes in antioxidant enzymes, germination capacity and vigour of tomato seeds in response of priming with polyamines

Afzal, Irfan; Munir, Faisal; Ayub, C. M.; Basra, S. M. A.; Hameed, Amjad; Nawaz, Aamir

doi:10.15258/sst.2009.37.3.23

Cited by 41 publications

(22 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The activity of catalase (AC) increased with the duration of priming [7,9]. The higher activity of catalase in seeds is supposed to be connected with the reinforcement of the defense against stress caused by free radicals [6,20].…”

Section: Resultsmentioning

confidence: 99%

“…However, the published data did not provide information that would indicate a correlation between the changing parameters of seed germinability and the selected physiological activities, which could be proposed as useful markers of seedsowing quality. This information is important in practice, among other things, to evaluate the results of seed priming, which is used for improving their germination, vigor, and seedling emergence -especially in unfavorable environmental conditions [7][8][9].…”

mentioning

confidence: 99%

See 1 more Smart Citation

Physiological Markers for Assessing Germinability of Lycopersicon esculentum Seeds Primed by Environment-Friendly Methods

Badek¹,

Romanowska-Duda²,

Grzesik³

et al. 2016

Pol. J. Environ. Stud.

View full text Add to dashboard Cite

Germinability of seeds is established during their development, maturation, and pre-sowing processing.Evaluating the germination capacity and identifying high-performance seeds are important incentives toward successful crop production. A significant problem during their quality evaluation is the lack of a single, rapid, and universally used marker for assessing the physiological potential of seeds of a given species. A reliable physiological marker would be a faster alternative than Pol. J. Environ. Stud. Vol. 25, No. 5 (2016), 1831-1838 AbstractCorrelations between the sowing value of primed tomato seeds (mean germination time -MGT, germination uniformity -T 75-25 ) and selected physiological events (total activity of dehydrogenases -TAD, activity of catalase -AC, activity of the cell cycle -ACC, and leakage of electrolytes -LE) were analyzed in order to find useful physiological markers of seed quality. To accomplish this purpose, we analyzed the effects of different environment-friendly priming methods, the levels of seed moisture content (MC), and incubation times during priming on germination properties (MGT, ) in relation to TAD, AC, ACC, and LE. In addition, we evaluated the effects of used seed conditioning methods on the growth and development of plants obtained from these seeds.The results showed positive effects of seed conditioning on the growth and development of plants obtained from these seeds, and that were significantly correlated with TAD, AC, ACC, and LE from the seeds. The correlations between MGT and the physiological parameters were the strongest (r 2 = 77.0 -97.2%). Somewhat lower values of the determination coefficient (r 2 = 62.8 -92.7%) were noted between T 75-25 and the physiological events. Therefore, the investigated physiological parameters of seed quality can be used as markers of germinability and sowing value reached by primed tomato seeds.

show abstract

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Physiological Markers for Assessing Germinability of Lycopersicon esculentum Seeds Primed by Environment-Friendly Methods

Badek¹,

Romanowska-Duda²,

Grzesik³

et al. 2016

Pol. J. Environ. Stud.

View full text Add to dashboard Cite

show abstract

“…Although there was slight increase in less vigor seeds due to priming process but no clear trend was noticed. Afzal et al (2009) reported that germination and seedling vigour can be enhanced by priming with Spermidine and Spermine in tomato cultivars through maintaining higher level of antioxidant enzymes like superoxide dismutase and catalase for eliminating excessive total peroxide. Saha et al (1990) reported that moisture equilibration soaking-drying treatment of soybean seeds increased the total dehydrogenase activity and amylase activity (0.48 to 0.53 and 33 to 35 µg starch hydrolyzed hour-1seed-1).…”

Section: Catalase Activitymentioning

confidence: 99%

“…It is a useful technique to exploit seed potential in arid and desert ecosystem. Seed priming is a low cost technology in which controlled hydration of seeds followed by re-drying is done to break dormancy, improve germination and stand establishment (Afzal et al, 2009). Another benefit of seed priming is that damaged and poor quality seeds float when they are soaked during priming, making it easier for farmers to discard low quality seeds (Farooq and Basra, 2006).…”

Section: Introductionmentioning

confidence: 99%

Effect of Seed Priming on Biochemical Changes in Fresh and Aged Seeds of Cucumber

Pandey

Umesha²

2017

JAS

View full text Add to dashboard Cite

The present investigation was conducted at the laboratory of Seed Science and Technology, Indian Institute of Horticultural Research, Hessaraghatta, and Bangalore, India during March 2014 to September 2014. The objective of the study was to identify effect of seed priming on biochemical changes in cucumber seeds. This study showed that the enhancement in seed viability and vigor in primed seeds was due to low membrane injury coupled with high enzyme activities (dehydrogenase and amylase). respectively in cucumber. Similarly, higher amylase activity was noticed upon priming in low vigor seeds These are to the extent of 2.1, 1.94 folds higher due to priming with K 2 HPO 4 10 -3 M and KH 2 PO 4 10 -3 M, respectively, in cucumber and 1.4 & 1.4 folds higher due to priming with K 2 HPO 4 10 -3 M .Alteration in protein profiles in low vigor seeds compared to high vigor seeds was noticed in the crops. The disappearance of some low molecular weight proteins in low vigor seeds might be the reason for lesser performance of these seeds. Seed priming although not restored all the lost proteins, but induced synthesis of some other proteins which probably might helped in restoration of germination ability and vigor. Esterase Isozyme profiles also showed variation in their number and intensity in less vigor seeds. ISSN 2166-0379 2017 http://jas.macrothink.org Journal of Agricultural Studies 63Primed seeds further exhibited tolerance to abiotic stress condition (high temperature and water stress) as evident from higher germination compared to unprimed. Besides these studies, some advanced studies were initiated in the present investigation to identify changes in proteome profiles and isocitratelyase gene expression in high vigor and low vigor seeds during germination.

show abstract

“…wheat (Iqbal and Ashraf, 2005;Farooq et al, 2011), sunflower (Farooq et al, 2007), rice (Farooq et al, 2008) and tomato (Afzal et al, 2009). Nonetheless, no study has been reported to evaluate the effects of polyamine seed priming on hot pepper.…”

Section: Scientific Notementioning

confidence: 99%

Exogenous Application of Polyamines Improves Germination and Early Seedling Growth of Hot Pepper

Khan

Ziaf

Amjad

et al. 2012

Chilean J. Agric. Res.

View full text Add to dashboard Cite

Polyamines are low molecular weight organic compounds involved in diverse range of biological processes in all living organisms. Seed priming is a technique that improves seed performance by rapid and uniform germination with normal and vigorous seedlings. A laboratory study was performed to explore the benefits of seed priming with polyamines on seed germination and seedling growth of hot pepper (Capsicum annuum L.) Hot pepper seeds were primed in aerated solution of putrescine, spermine, or spermidine (25, 50, 75, and 100 mM) for 48 h at 25 ± 2 °C. Significant results (P < 0.05) were observed for different attributes of seed germination and early seedling growth. Polyamines priming resulted in earlier and synchronized germination via improving final germination percentage, time to 50% germination, mean germination time, germination energy, germination speed and germination index compared with control. Improvement in shoot and root length, seedling fresh and dry weight, seedling growth rate and seedling vigor index was clearly indicative of positive effects of seed priming with polyamines. Out of all polyamines, putrescine proved to be better at low concentrations (i.e. 25 and 50 mM) for most of the traits related to seed emergence and seedling growth.

show abstract

Changes in antioxidant enzymes, germination capacity and vigour of tomato seeds in response of priming with polyamines

Cited by 41 publications

References 10 publications

Physiological Markers for Assessing Germinability of Lycopersicon esculentum Seeds Primed by Environment-Friendly Methods

Physiological Markers for Assessing Germinability of Lycopersicon esculentum Seeds Primed by Environment-Friendly Methods

Effect of Seed Priming on Biochemical Changes in Fresh and Aged Seeds of Cucumber

Exogenous Application of Polyamines Improves Germination and Early Seedling Growth of Hot Pepper

Contact Info

Product

Resources

About