Changes in antioxidant activity in sub-cellular fractions of tolerant and susceptible wheat genotypes in response to long term salt stress

Sairam, R. K.; Srivastava, G. C.

doi:10.1016/s0168-9452(02)00037-7

Cited by 439 publications

(226 citation statements)

References 42 publications

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“…However, compared to control, lipid peroxidation rate remain at basal level at lower concentration of elicitor treatments. Interestingly, very high amount of MDA content was measured in the leaves treated with K 2 HPO 4 (50 mM) which coincide with the earlier reports, where increased amount of MDA production was observed in wheat, cotton, rice and alfalfa (Sairam and Srivastava 2002;Diego et al 2003;Tijen and Ìsmail 2005;Wang and Han 2007) under higher concentrations of salts. Although other treatments including CaCl 2 (0.5 %) and INA (2.5 mM) did not show significant change of MDA content compared to control.…”

Section: Discussionsupporting

confidence: 89%

Abiotic elicitors mediated elicitation of innate immunity in tomato: an ex vivo comparison

Chakraborty

Ghosh²,

Chandra

et al. 2016

Physiol Mol Biol Plants

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Improvement of the host resistance by using hazard free chemical elicitors is emerging as an alternative approach in the field of plant disease management. In our present work, we have screened the efficacy and possible mechanism of abiogenic elicitors like Dipotassium hydrogen orthophosphate (K 2 HPO 4 ), Oxalic acid (OA), Isonicotinic acid (INA), Salicylic acid (SA), Acetylsalicylate (AS), Arachidonic acid (AA) and Calcium chloride (CaCl 2 ) to stimulate innate immune responses in Lycopersicum esculentum Mill. Excised tomato leaves, treated with elicitors at three different concentrations, were found to stimulate defense and antioxidative enzymes, total phenol and flavonoid content after 24 h of incubation. CaCl 2 (0.5 %) followed by INA (2.5 mM) were found most effective in activation of all such defense molecules in tomato leaves. Furthermore, nitric oxide (NO), a key gaseous mediator in plant defense signaling, was also measured after subsequent elicitor application. Higher doses of elicitors showed an elevated level of reactive oxygen species (ROS) generation, enhanced lipid peroxidation rate and proline content, which indicates the extent of abiotic stress generation on the leaves. However, ROS production, lipid peroxidation rate and proline concentration remain significantly reduced as a result of CaCl 2 (0.5 %) and INA (2.5 mM) application. A sharp increase of total chlorophyll content was also recorded due to treatment of CaCl 2 (0.5 %). These results demonstrate the effects of different abiogenic elicitors to regulate the production of defense molecules. Results also suggest that among all such chemicals, CaCl 2 (0.5 %) and INA (2.5 mM) can be used as a potential elicitor in organic farming of tomato.

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Section: Discussionsupporting

confidence: 89%

Abiotic elicitors mediated elicitation of innate immunity in tomato: an ex vivo comparison

Chakraborty

Ghosh²,

Chandra

et al. 2016

Physiol Mol Biol Plants

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“…An increase in the activity of superoxide dismutase, ascorbate peroxidase, glutathione reductase, catalase, and peroxidases in response to salinity stress, as well as higher antioxidant activity in tolerant species/varieties, have been reported (Abreu et al, 2008;Meloni et al, 2003;Sairam and Srivastava, 2002;Smirnoff, 2005). Our results suggest that the reduction of lipid peroxidation in salinized GB-treated plants could be explained in part by the enhanced SOD activity induced by GB.…”

Section: Discussionmentioning

confidence: 99%

Glycinebetaine improves salt tolerance in vinal (Prosopis ruscifolia Griesbach) seedlings

Meloni

Martínez

2009

Braz. J. Plant Physiol.

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Glycinebetaine (GB) is a very important organic osmolyte that accumulates in a number of diverse groups of plants in response to environmental stress. In some plants, increased resistance to drought, salinity and low temperature has been achieved through exogenous application of GB. In this study, the effect of exogenously applied GB (8 mM) on the ability of vinal (Prosopis ruscifolia G.) plants to withstand NaCl stress was investigated. The dry biomass of vinal showed a decrease under salt stress, but in GBtreated plants exposed to the same stress, this reduction was mitigated. Sodium accumulated in the leaves of plants grown under saline conditions, but the addition of GB to salt-grown plants reduced Na + content by 40%. Salinity significantly reduced the K + concentration in leaves to 65% that of non-salinized controls. In the presence of GB, leaf K + was comparatively higher, reaching as much as 90% of the control concentration. The sodium: potassium ratio in leaves was significantly higher in salt-stressed plants, but this ratio was lowered significantly by the addition of GB. When compared to control plants, NaCl stress increased malondialdehyde (MDA) concentrations by 95%, but GB application reduced the MDA concentration in these same NaCl-treated plants. In comparison to control plants, the activity of superoxide dismutase (SOD) increased by 52% in salt-stressed plants. The addition of GB to salttreated plants stimulated SOD activity twice that of the non-salizined control. These results suggest that, in addition to protecting membranes, GB-enhanced salinity tolerance in vinal may involve an antioxidant mechanism involving enhanced SOD activity and improving the ion homeostasis under conditions of high salinity.Key words: lipid peroxidation, salt stress, superoxide dismutase. resUMo glycinabetaine incrementa a tolerância a estresse salino em plântulas de vinal (Prosopis ruscifolia griesbach): Glycinabetaína (GB) é um dos osmólitos orgânicos mais importantes que se acumulam em diversas plantas em resposta a estresses ambientais. Em algumas espécies, o aumento a resistência à seca, salinidade ou baixa temperatura foram conseguidos pela aplicação exógena da GB. Neste estudo foi investigado o efeito de GB aplicado exogenamente (8 mM) na habilidade do vinal (Prosopis ruscifolia Griesbach) para suportar o estresse salino induzido por NaCl. A biomassa seca das plantas diminuiu por efeito do tratamento salino, mas aumentou em plantas tratadas com GB. O Sódio (Na + ) acumulou-se nas folhas das plantas crescidas sob estresse salino, mas a adição do GB às plantas tratadas com NaCl provocou uma diminuição de 40% no conteúdo de Na + . Em comparação às plantas controle, nas plantas tratadas com NaCl observou-se uma redução de 65% no conteúdo de potássio (K + ). Na presença do GB, a concentração de K + nas plantas sob estresse salino foi maior que nas plantas não tratadas com GB. A relação sódio: potássio aumentou significativamente Braz. J. Plant Physiol., 21(3): 233-241, 2009 234 DIEGO A. MELONI and CARLOS A....

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“…A coloração de segmentos radiculares com reagente de Shiff confi rmou este resultado, já que este reagente evidencia aldeídos, os quais são subprodutos da peroxidação de lipídios (Yamamoto et al 2001). Considerando que a concentração de TBARS tem sido usada como indicador de danos oxidativos (Dionisio-Sese & Tobita 1998;Sairam & Srivastava 2002;Bandeoglu et al 2004), é possível que a salinidade não tenha acarretado o estresse oxidativo secun-dário em raízes de feijão caupi. Assim sendo, esta espécie deve apresentar mecanismos efi cientes para a renovação dos lipídios de membrana afetados e/ou para a remoção das EROs produzidas.…”

Section: Discussionunclassified

Atividade de enzimas antioxidantes e inibição do crescimento radicular de feijão caupi sob diferentes níveis de salinidade

et al. 2012

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ABSTRACT(Activities of antioxidant enzymes and root growth inhibition in cowpea seedlings exposed to diff erent salt levels). Phenol peroxidase (POX) is a dual enzyme that is involved with hydrogen peroxide scavenging and lignin biosynthesis, contributing to growth inhibition by secondary wall thickening. In order to relate growth inhibition to salt-induced oxidative modulation, the activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and POX were evaluated in cowpea roots under salinity. Four-day-old seedlings of the Pitiúba and Pérola cultivars were exposed to 0, 25, 50, 75 and 100 mM NaCl in germination paper under controlled conditions. Aft er two days of treatment, root length was reduced under 100 mM NaCl by 56 and 26% in Pitiúba and Pérola, respectively, which was associated with enhanced electrolyte leakage and cell death in the root apex. NaCl salinity did not trigger lipid peroxidation, indicating that cell death was probably due to membrane damage instead of oxidative stress. Salt stress reduced the activity of SOD, CAT and APX and increased the POX activity, demonstrating that this enzyme plays a role in oxidative protection in cowpea roots exposed to NaCl salinity. In conclusion, salt-induced growth inhibition in cowpea roots could be attributed, at least in part, to a coordinate action involving an increase in POX activity and a drop in CAT and APX activities. Atividade de enzimas antioxidantes e inibição do crescimento radicular de feijão caupi sob diferentes níveis de salinidade

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Changes in antioxidant activity in sub-cellular fractions of tolerant and susceptible wheat genotypes in response to long term salt stress

Cited by 439 publications

References 42 publications

Abiotic elicitors mediated elicitation of innate immunity in tomato: an ex vivo comparison

Abiotic elicitors mediated elicitation of innate immunity in tomato: an ex vivo comparison

Glycinebetaine improves salt tolerance in vinal (Prosopis ruscifolia Griesbach) seedlings

Atividade de enzimas antioxidantes e inibição do crescimento radicular de feijão caupi sob diferentes níveis de salinidade

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