Changes in antigenic profile during culture of Neoparamoeba sp., causative agent of amoebic gill disease in Atlantic salmon

Villavedra, M; Mccarthy, K L; To, Joyce; Morrison, Richard N.; Crosbie, Philip; Broady, Kevin W.; Raison, Robert L.

doi:10.1016/j.ijpara.2005.05.014

Cited by 4 publications

(7 citation statements)

References 21 publications

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“…1985). In fact, the large majority of the mAbs cross‐reactive between the cultured and WT parasites recognized epitopes not susceptible to this periodate oxidation (data not shown) as did the majority (80%) of the cross‐reactive mAbs obtained after immunization with non‐infective parasites (Villavedra et al. 2005).…”

Section: Discussionmentioning

confidence: 97%

“…PA027 is a non‐infectious clone of N. pemaquidensis , originally isolated from AGD‐affected salmon and established in continuous culture since 1994. These parasites were grown on malt–yeast–seawater agar plates as described in Villavedra et al. (2005).…”

Section: Methodsmentioning

confidence: 99%

“…Experimental infections of AGD can only be established by cohabitation with infected fish or by exposure to parasites freshly isolated from infected fish (Zilberg, Gross & Munday 2001). The lack of ability to infect naïve salmon was shown to correlate with changes in antigen expression, particularly at the surface of the parasite during the time in culture (Villavedra, McCarthy, To, Morrison, Crosbie, Broady & Raison 2005).…”

Section: Introductionmentioning

confidence: 99%

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Carbohydrate epitopes are immunodominant at the surface of infectious Neoparamoeba spp.

Villavedra

Lemke

et al. 2007

Journal of Fish Diseases

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Amoebic gill disease, the main disease of concern to the salmon industry in Tasmania, is caused by the amoeba, Neoparamoeba spp. Experimental infection can only be induced by exposure to wild-type (WT) parasites isolated from the gills of infected fish, as cultured amoebae are non-infective. To characterize the surface antigens of WT parasites, we produced monoclonal antibodies (mAbs) using subtractive immunization. Mice inoculated with non-infective parasites were treated with cyclophosphamide, to deplete reactive lymphocytes, and then immunized with different antigen preparations from infective parasites. When whole parasites were used for boosting, the percentage of WT unique mAbs was very high (86%) as was the percentage of mAbs specific for carbohydrate epitopes (89%). When deglycosylated membranes were used, the numbers of mAbs specific for non-carbohydrate epitopes did not increase, but the total number of WT unique mAbs was reduced (86-40%). Using an untreated membrane preparation, the total number of mAbs to surface molecules was very high, but all recognized carbohydrate epitopes. The total number of mAbs recognizing carbohydrate epitopes on the surface of the WT parasites was 97%, suggesting that the dominant epitopes on the surface molecules unique to WT parasites are carbohydrate in nature.

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Section: Discussionmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Carbohydrate epitopes are immunodominant at the surface of infectious Neoparamoeba spp.

Villavedra

Lemke

et al. 2007

Journal of Fish Diseases

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show abstract

“…Amoebae 2.1.1. Non-infectious N. pemaquidensis N. pemaquidensis, (designated PAO27) originally isolated and cloned from AGD affected salmon was grown on malt-yeastseawater agar plates as described in Villavedra et al [5]. This culture was first established in 1994 and is non-infectious.…”

Section: Methodsmentioning

confidence: 99%

“…The first and most obvious in light of the work of Young et al [2] is that neither N. pemaquidensis nor N. branchiphila are aetiological agents of AGD, and overgrow N. perurans in culture. Another explanation is that when the parasites are being cultured in vitro important changes occur in the expression of surface molecules that are crucial for the infective capability of the parasite [5].…”

Section: Introductionmentioning

confidence: 99%