Changes in antifungal defence systems during the intermoult period in the Colorado potato beetle

Tomilova, Oksana G.; Yaroslavtseva, O. N.; Ganina, Mariya D.; Tyurin, M. V.; Chernyak, E. I.; Senderskiy, Igor V.; Noskov, Yury A.; Polenogova, Olga; Akhanaev, Yuriy; Kryukov, V. Yu.; Глупов, В. В.; Морозов, С. В.

doi:10.1016/j.jinsphys.2019.05.003

Cited by 21 publications

(20 citation statements)

References 80 publications

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“…The enhancement of PO is observed under development of various bacterioses and caused by damages of insect’s tissues as well as by recognition of bacterial cell wall compounds, formation of hemocyte nodules and their melanization (Bidla et al, 2009; Tokura et al, 2014; Dubovskiy et al, 2016). An increase in GST under mycoses usually correlates with the severity of the infectious process (Dubovskiy et al, 2012; Tomilova et al, 2019) and confirms the results obtained by Butt et al (2013) when studying the pathogenesis of M. brunneum in Ae. aegypti larvae.…”

Section: Discussionsupporting

confidence: 82%

Combined effect of the entomopathogenic fungusMetarhizium robertsiiand avermectins on the survival and immune response ofAedes aegyptilarvae

Noskov

Polenogova

Yaroslavtseva

et al. 2019

PeerJ

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Combination of insect pathogenic fungi and microbial metabolites is a prospective method for mosquito control. The effect of the entomopathogenic fungus Metarhizium robertsii J.F. Bischoff, S.A. Rehner & Humber and avermectins on the survival and physiological parameters of Aedes aegypti (Linnaeus, 1762) larvae (dopamine concentration, glutathione S-transferase (GST), nonspecific esterases (EST), acid proteases, lysozyme-like, phenoloxidase (PO) activities) was studied. It is shown that the combination of these agents leads to a synergistic effect on mosquito mortality. Colonization of Ae. aegypti larvae by hyphal bodies following water inoculation with conidia is shown for the first time. The larvae affected by fungi are characterized by a decrease in PO and dopamine levels. In the initial stages of toxicosis and/or fungal infection (12 h posttreatment), increases in the activity of insect detoxifying enzymes (GST and EST) and acid proteases are observed after monotreatments, and these increases are suppressed after combined treatment with the fungus and avermectins. Lysozyme-like activity is also most strongly suppressed under combined treatment with the fungus and avermectins in the early stages posttreatment (12 h). Forty-eight hours posttreatment, we observe increases in GST, EST, acid proteases, and lysozyme-like activities under the influence of the fungus and/or avermectins. The larvae affected by avermectins accumulate lower levels of conidia than avermectin-free larvae. On the other hand, a burst of bacterial CFUs is observed under treatment with both the fungus and avermectins. We suggest that disturbance of the responses of the immune and detoxifying systems under the combined treatment and the development of opportunistic bacteria may be among the causes of the synergistic effect.

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Section: Discussionsupporting

confidence: 82%

Combined effect of the entomopathogenic fungusMetarhizium robertsiiand avermectins on the survival and immune response ofAedes aegyptilarvae

Noskov

Polenogova

Yaroslavtseva

et al. 2019

PeerJ

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“…Moreover, the inhibition of esterase activity may increase insect susceptibility to bacterial toxins 35 . Thus, blocking detoxification enzyme activation in Colorado potato beetle gut by the fungus may lead to increased toxic effects of avermectins, which may lead to general physiological consequences, such as a delay in development, weakening of cellular immunity and changes in the thickness and biochemical properties of integuments 39,40,69 . These disruptions are crucial to the susceptibility of Colorado potato beetle to Metarhizium and Beauveria 69,70 .…”

Section: Discussionmentioning

confidence: 99%

“…Thus, blocking detoxification enzyme activation in Colorado potato beetle gut by the fungus may lead to increased toxic effects of avermectins, which may lead to general physiological consequences, such as a delay in development, weakening of cellular immunity and changes in the thickness and biochemical properties of integuments 39,40,69 . These disruptions are crucial to the susceptibility of Colorado potato beetle to Metarhizium and Beauveria 69,70 . It is likely that both direct and inverse effects between fungal infection and avermectins may occur in the investigated system and may lead to the formation of a "vicious circle" in which avermectins promote fungal infection (in the cuticle and hemolymph), and this infection increases the toxic effect of avermectins (in the gut).…”

Section: Discussionmentioning

confidence: 99%

Fungus Metarhizium robertsii and neurotoxic insecticide affect gut immunity and microbiota in Colorado potato beetles

Kryukov

Rotskaya

Yaroslavtseva

et al. 2021

Sci Rep

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Fungal infections and toxicoses caused by insecticides may alter microbial communities and immune responses in the insect gut. We investigated the effects of Metarhizium robertsii fungus and avermectins on the midgut physiology of Colorado potato beetle larvae. We analyzed changes in the bacterial community, immunity- and stress-related gene expression, reactive oxygen species (ROS) production, and detoxification enzyme activity in response to topical infection with the M. robertsii fungus, oral administration of avermectins, and a combination of the two treatments. Avermectin treatment led to a reduction in microbiota diversity and an enhancement in the abundance of enterobacteria, and these changes were followed by the downregulation of Stat and Hsp90, upregulation of transcription factors for the Toll and IMD pathways and activation of detoxification enzymes. Fungal infection also led to a decrease in microbiota diversity, although the changes in community structure were not significant, except for the enhancement of Serratia. Fungal infection decreased the production of ROS but did not affect the gene expression of the immune pathways. In the combined treatment, fungal infection inhibited the activation of detoxification enzymes and prevented the downregulation of the JAK-STAT pathway caused by avermectins. The results of this study suggest that fungal infection modulates physiological responses to avermectins and that fungal infection may increase avermectin toxicosis by blocking detoxification enzymes in the gut.

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“…We found that the synergy is caused by changes in cellular and humoral immunity responses, gut microbiota, morphological and biochemical properties of integuments that stipulate fungal adhesion, penetration and colonization. We also suggest that the disturbance in larval development caused by these metabolites and bacteria has a great impact on immunity, integument properties and susceptibility to fungi [14]. The investigated combinations provide a stable synergy in laboratory and field experiments.…”

Section: Multicomponent Approaches In Biological Controlmentioning

confidence: 79%

“…Interestingly, viral pathogenesis does not depend on the phenoloxidase activity that was shown on L. dispar larvae using several lines of evidence [13]. The larvae of CPB demonstrated strong differences in susceptibility to the fungus Metarhizium during the intermolt period of the last instar due to the changes in integument thickness and epicuticular hydrocarbon contents (which determine the level of conidial adhesion) as well as changes in cellular immunity [14]. The solitaria phase of migratory locust was more resistant to fungi (Metarhizium and Beauveria) than to gregaria [15].…”

Section: Physiological State Of Hostsmentioning

confidence: 91%

Insect parasites in multicomponent systems and development of new bioinsecticides

2020

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Stable and dynamic interactions among plants, herbivorous insects, parasites and associated microbes are formed in natural habitats. The study of these interactions in multicomponent models is required to develop integrated methods for the management of insect pest populations. In this work, we summarize our studies on the influence of different factors, such as hygrothermal conditions, host development, host microbiota, plant quality, and concomitant infections, on interactions between insects and their parasites, such as fungi, bacteria, viruses and parasitoids. Some approaches for developing complex products for biocontrol are also discussed. For example, the use of natural compounds with immunosuppressive effects may enhance the efficacy of microbial agents toward pest insects.

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Changes in antifungal defence systems during the intermoult period in the Colorado potato beetle

Cited by 21 publications

References 80 publications

Combined effect of the entomopathogenic fungusMetarhizium robertsiiand avermectins on the survival and immune response ofAedes aegyptilarvae

Combined effect of the entomopathogenic fungusMetarhizium robertsiiand avermectins on the survival and immune response ofAedes aegyptilarvae

Fungus Metarhizium robertsii and neurotoxic insecticide affect gut immunity and microbiota in Colorado potato beetles

Insect parasites in multicomponent systems and development of new bioinsecticides

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