Changes in anthocyanin concentration and composition in ‘Jaguar’ rose flowers due to transient high-temperature conditions

Dela, G.D. Gal; Or, Etti; Ovadia, Rinat; Nissim-Levi, Ada; Weiss, David; Oren-Shamir, Michal

doi:10.1016/s0168-9452(02)00417-x

Cited by 164 publications

(120 citation statements)

References 27 publications

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“…'Kardinal') was developmentally regulated and paralleled anthocyanin production in petals (Tanaka et al 1995). Also, in the rose cultivar 'Jaguar', the decreased anthocyanin concentration in flower buds under transient high-temperature conditions was due in part to reduced transcription of DFR (Dela et al 2003). According to these reports and our results, DFR seems to be a key enzyme in anthocyanin biosynthesis pathway of rose plants.…”

Section: Expression Analysis Of Anthocyanin Biosynthesis Related Genessupporting

confidence: 79%

Differential expression of anthocyanin biosynthesis genes in suspension culture cells of Rosa hybrida cv. Charleston

Hennayake

Takagi

Nishimura

et al. 2006

Plant Biotechnology

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A suspension cultured cell line was established from the cultivar of Rosa hybrida 'Charleston' as a study model to understand the response of the anthocyanin biosynthesis pathway to environmental cues. The major identified anthocyanin in cell cultures was cyanidin 3-glucoside (chrysanthemin). The anthocyanin yield was enhanced by culturing cells in the EM medium with added sucrose at high concentration under additional UV-B radiation to white light. Three cDNA fragments were cloned with degenerate primers by RT-PCR and the obtained sequences shared high homology with putative key enzymes (DFR, ANS, and UF3GT) of other species. The expression levels of these three genes were promoted under optimum conditions for anthocyanin accumulation. These results suggest that expression levels of these genes were closely correlated with a temporal buildup of anthocyanins in response to environmental factors.Key words: Anthocyanin, chrysanthemin, cyanin, Rosa hybrida, suspension cell culture.Plant Biotechnology 23, 379-385 (2006) Original PaperThis article can be found at http://www.jspcmb.jp/ pelargonidin, cyanidin and peonidin type anthocyanins (Biolley et al. 1994). Recently, tissue culturing has become an important biotechnological technique to produce various secondary metabolites including anthocyanins on a mass scale for industrial and academic interest (Hirner et al. 2001;Gantet and Memelink 2002). Despite the fact that some plant species have been shown to produce anthocyanins in cell cultures, little information of the cell culture techniques that are capable of producing in vitro anthocyanins has been reported in rose.The biosynthesis of anthocyanins in plant cell culture is stimulated by not only genetic factors but also environmental factors such as light, temperature, growth regulators and nutrition (Sato et al. 1996;Zhang et al. 1997;Zhang et al. 2002). The identity of the environmental stimuli that lead to the accumulation of anthocyanins in rose is of considerable interest. To date, Sato et al. (1996) has examined the intensity of white light that induces anthocyanin production in strawberry cell culture lines; however, no information was obtained about the effect of light quality on the production of anthocyanins in cultural cells. Therefore, the originality of this research is to investigate anthocyanin biosynthesis in cultural cells exposed to UV-B (290-320 nm) radiation.In this study, we used Rosa hybrida cultivar 'Charleston', a climbing type, originated in Australia. The open flowers of 'Charleston' undergo a striking color change from yellow to red over 10-12 days under natural daylight. We established a suspension cultured cell line from this cultivar of rose. Anthocyanin content was quantified by HPLC to determine the most efficient conditions, such as light including UV-B radiation and media, for continuous production of the pigments in the rose cell suspension. The expression levels of three genes encoding putative key enzymes (DFR, ANS, and UF3GT) for anthocyanin biosynthesis were ana...

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Section: Expression Analysis Of Anthocyanin Biosynthesis Related Genessupporting

confidence: 79%

Differential expression of anthocyanin biosynthesis genes in suspension culture cells of Rosa hybrida cv. Charleston

Hennayake

Takagi

Nishimura

et al. 2006

Plant Biotechnology

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“…Previous reports indicated that light and temperature are important environmental factors that affect flavonoid biosynthesis in many plants (Bowler and Chua, 1994;Christie and Jenkins, 1996;Christie et al, 1994;Dela et al, 2003;Ubi et al, 2006). In many grape accessions, such as black grapes 'Pione' and 'Kyoho', and red grape 'Aki Queen', the accumulation of anthocyanins in berry skin begins after the onset of ripening (veraison) and is affected by light and temperature conditions (Azuma et al, 2012;Kliewer and Torres, 1972;Matus et al, 2009;Tomana et al, 1979a, b;Yamane et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Microarray Analysis for the Screening of Genes Inducible by Light or Low Temperature in Post-veraison Grape Berries

et al. 2015

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Flavonoid biosynthesis in grape (Vitis spp.) berry skin is affected by environmental factors such as light and temperature. However, the components of the light signaling and low-temperature-induced abscisic acid (ABA) signaling networks related to flavonoid accumulation in grape berry skin have not been fully elucidated, and how environmental conditions affect these components remains unclear. To clarify the details of the possible light-and ABA-related signal transduction networks, we developed a grape oligo-DNA microarray (38,549 independent probes) using the publicly available genomic sequence of grape, and performed comprehensive transcriptome analysis using detached 'Pione' grape (Vitis labruscana × V. vinifera) berries cultured under different light and temperature conditions. Using the microarray data, we explored the light-inducible and low-temperature-inducible genes in post-veraison grape berries. We identified 40 lightinducible genes, 55 low-temperature-inducible genes, and 34 genes induced by light plus low temperature. Among these, we selected elongated hypocotyl 5 (HY5), open stomata 1 (OST1), and enhanced response to ABA 1 (ERA1) as candidate light-inducible, low-temperature-inducible, and light-plus low-temperature-inducible genes, respectively. We investigated their detailed expression characteristics in grape accessions by means of quantitative real-time polymerase chain reaction analyses, and hypothesized that HY5, OST1, and ERA1 might be involved in flavonoid biosynthesis via light signaling and low-temperature signaling networks. We also established an extensive catalog of gene expression patterns to support future investigations of other candidate genes that respond to light and temperature in grape berry skin.

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“…Several cis-acting elements in the promoter of key genes regulating anthocyanin biosynthesis are activated by a series of signal transduction processes under the light and temperature receptors (Vitrac et al, 2000;Dela et al, 2003;Zhang et al, 2003). Transposon insertions and deletions in the promoter region of grape 1 This work was supported by the Doctoral Program Special Fund of the Ministry of Education in China (grant no.…”

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confidence: 99%

The Methylation of the PcMYB10 Promoter Is Associated with Green-Skinned Sport in Max Red Bartlett Pear

et al. 2013

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Varieties of the European pear (Pyrus communis) can produce trees with both red-and green-skinned fruits, such as the Max Red Bartlett (MRB) variety, although little is known about the mechanism behind this differential pigmentation. In this study, we investigated the pigmentation of MRB and its green-skinned sport (MRB-G). The results suggest that a reduction in anthocyanin concentration causes the MRB-G sport. Transcript levels of PcUFGT (for UDP-glucose:flavonoid 3-O-glucosyltransferase), the key structural gene in anthocyanin biosynthesis, paralleled the change of anthocyanin concentration in both MRB and MRB-G fruit. We cloned the PcMYB10 gene, a transcription factor associated with the promoter of PcUFGT. An investigation of the 2-kb region upstream of the ATG translation start site of PcMYB10 showed the regions 2604 to 2911 bp and 21,218 to 21,649 bp to be highly methylated. A comparison of the PcMYB10 promoter methylation level between the MRB and MRB-G forms indicated a correlation between hypermethylation and the green-skin phenotype. An Agrobacterium tumefaciens infiltration assay was conducted on young MRB fruits by using a plasmid constructed to silence endogenous PcMYB10 via DNA methylation. The infiltrated fruits showed blocked anthocyanin biosynthesis, higher methylation of the PcMYB10 promoter, and lower expression of PcMYB10 and PcUFGT. We suggest that the methylation level of PcMYB10 is associated with the formation of the greenskinned sport in the MRB pear. The potential mechanism behind the regulation of anthocyanin biosynthesis is discussed.

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Changes in anthocyanin concentration and composition in ‘Jaguar’ rose flowers due to transient high-temperature conditions

Cited by 164 publications

References 27 publications

Differential expression of anthocyanin biosynthesis genes in suspension culture cells of Rosa hybrida cv. Charleston

Differential expression of anthocyanin biosynthesis genes in suspension culture cells of Rosa hybrida cv. Charleston

Microarray Analysis for the Screening of Genes Inducible by Light or Low Temperature in Post-veraison Grape Berries

The Methylation of the PcMYB10 Promoter Is Associated with Green-Skinned Sport in Max Red Bartlett Pear

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