Changes in amino-acid composition of developing rat incisor enamel

Robinson, C.; Lowe, Norman R.; Weatherell, J.A.

doi:10.1007/bf02012762

Cited by 95 publications

(56 citation statements)

References 16 publications

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“…Previous studies suggested that the first product secreted by ameloblasts is enriched with enamelins (Farge et al 1987;Deutsch et al 1984;Robinson et al 1977). According to this suggestion, enamelins would be expected at the interface between dentine and enamel, as we report in the present study.…”

Section: Discussionsupporting

confidence: 83%

Immunohistochemical Similarities and Differences between Amelogenin and Tuftelin Gene Products During Tooth Development

Diekwisch¹,

Ware²,

Fincham

et al. 1997

J Histochem Cytochem.

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SUMMARYAmelogenins and tuftelins are highly specialized proteins secreted into the developing enamel matrix during mammalian enamel formation. Both tuftelins and amelogenins have been associated with various functions during nucleation and maturation of the developing enamel matrix. In this study we conducted experiments to investigate whether tuftelins and portions of the amelogenin molecule were deposited and processed in spatially distinguished portions of the developing enamel matrix, using antibodies specific against tuftelin or amelogenins. The amelogenin antibodies were raised against recombinant and native amelogenins and also included an antibody against a polypeptide encoded by amelogenin exon 4. To compare spatial expression patterns of enamel protein epitopes, 3-day postnatal mouse molar tooth organs were processed for paraffin histology and cut into serial sections. Adjacent sections were exposed to antibodies against either tuftelin or various amelogenin epitopes. To investigate age-related changes of enamel protein expression, amelogenin and tuftelin antibodies were applied to tooth organs of developmental stages E19 and 1, 3, 5, 7, 9 and 11 postnatal days. Tuftelin was detected within the odontoblast processes during earlier stages of development (E19 and 1 day postnatal), whereas during later stages (3-11 days) it was recognized in a portion of the enamel layer adjacent to the dentine-enamel junction. In contrast, all four antibodies against amelogenins reacted with parts of the ameloblast cytoplasm and the entire enamel layer. Using immunohistochemistry, we were not able to detect any differences in the spatial distribution of the four amelogenin epitopes investigated. The spatial differences in the distribution of amelogenin and tuftelin as observed in this study may be intepreted as an indication of functional differences between both proteins during early enamel biomineralization.

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Section: Discussionsupporting

confidence: 83%

Immunohistochemical Similarities and Differences between Amelogenin and Tuftelin Gene Products During Tooth Development

Diekwisch¹,

Ware²,

Fincham

et al. 1997

J Histochem Cytochem.

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“…Biochemical studies have shown the beginning of a decrease of protein and a change of the amino acid composition of the enamel matrix during the formative stage of amelogenesis in human and rat teeth [Fukae and Shimizu, 1974;Robinson et al, 1977Robinson et al, , 1981, A close relation with ameloblast cell morphology, however, remains uncertain. Secretory ameloblasts may, therefore, func tion in the resorption of the enamel matrix as well as in matrix formation.…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural and Cytochemical Studies of Resorptive and Digestive Functions of Secretory Ameloblasts in Kitten Tooth Germs

Sasaki¹

1983

Cells Tissues Organs

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Ultrastructural and cytochemical studies of kitten secretory ameloblasts were made in order to clarify their functions in the resorption and digestion of extracellular organic materials. The secretory ameloblast had triangular Tomes’ processes whose profile was divided into type 1 and type 2 faces. Type 1 face was associated with tubular structures, coated pits, coated vesicles, and irregularly shaped vesicles presumably representing phago-somes. Freeze-fracture replicas clearly showed the presence of large, particle-rich depressions and small depressions on the cell membrane P face in the type 1 face of the Tomes’ process. Exocytosis of secretory granules was seldom observed. In both thin sections and replicas, the type 2 face possessed cell membrane microinvaginations. From the supranuclear region to a zone near the Tomes’ process, many dense bodies, multivesicular bodies, and vacuoles were present; and many of them showed intense acid phosphatase reactions. Reaction products of acid phosphatase were demonstrated in the Golgi apparatus, GERL, and the lateral cell membrane. These results suggest that kitten secretory ameloblasts resorb and digest extracellular organic materials.

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“…the relatively larger amounts of protein in the enamel beneath the fissure and sometimes in cuspal regions [Robinson et al, 1971], could reflect the difficulty of withdrawing protein from morphologically complex regions where enamel prisms tend to intertwine. The high concentrations of tuft material at the ADJ (amelo-dentinal junction), on the other hand [Wheatherell et al, 1968;Robinson et al, 1971], could be due either to the production of greater amounts of 'mature' enamel protein which apparently occurs at an early stage of amelogenesis Robinson et al, 1977], or to the tendency for the material to migrate to the more porous interior enamel.…”

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confidence: 99%

Distribution of Magnesium in Mature Human Enamel

Robinson

Weatherell

Hallsworth³

1981

Caries Res

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The distribution of magnesium has been determined in the enamel of permanent human teeth. The concentrations increased from the surface towards the interior. The gradients were not smooth, pockets of relatively high magnesium concentration frequently occurred around the dentinal cornua and in the region of fissures. The distribution patterns obtained suggested a correlation between magnesium concentration and low density enamel and possibly between magnesium and protein concentration.

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Changes in amino-acid composition of developing rat incisor enamel

Cited by 95 publications

References 16 publications

Immunohistochemical Similarities and Differences between Amelogenin and Tuftelin Gene Products During Tooth Development

Immunohistochemical Similarities and Differences between Amelogenin and Tuftelin Gene Products During Tooth Development

Ultrastructural and Cytochemical Studies of Resorptive and Digestive Functions of Secretory Ameloblasts in Kitten Tooth Germs

Distribution of Magnesium in Mature Human Enamel

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