Changes in adenine nucleotides and lipid hydroperoxides during normothermic cardiopulmonary bypass in a porcine model of type II non-heart-beating donor

Arias-Dı́az, Javier; Alvarez, J.; Gómez, Miguel Ángel Sánchez; Barrio, Roberto; Garcia-Carreras, C.; González, Pablo; Jl, Balibrea

doi:10.1016/s0041-1345(97)01117-2

Cited by 26 publications

(18 citation statements)

References 7 publications

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“…Recently, the use of cardiopulmonary bypass as a period of time of warm perfusion before graft procurement (the so-called 'normothermic recirculation') has been demonstrated to improve the quality of these grafts, decreasing the I-R associated to the procedure of transplantation (8,12). The first ideas of NR technique were introduced by Hoshino et al (23) and then its usefulness was further demonstrated by our group and others (24,25). Thus, in NHBD procurement, NR seems to have a similar effect to that of ischemic preconditioning.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Normothermic Recirculation is Mediated by Ischemic Preconditioning in NHBD Liver Transplantation

Net¹,

Valero²,

Almenara³

et al. 2005

American Journal of Transplantation

112

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We have evaluated the involvement of hepatic preconditioning mediators (adenosine, adenosine A1 and A2 receptors) during normothermic recirculation (NR) in a model of liver transplantation from non-heart-beating donor (NHBD) pigs.Application of NR after 20 min of warm ischemia (WI) reversed the lethal injury associated with transplantation of NHBD livers (achieving 5-day survival and diminishing glutathione S-transferase (GST), aspartate aminotransferase (AST) and hyaluronic acid (HA)).Adenosine administration prior to WI simulated the effect of NR. Measuring adenosine, we found that during NR, hepatic adenosine levels increased and xanthine levels decreased. Then when we blocked A2 receptors the effect of NR was abolished, whereas the blocking of A1 receptors further protected the liver. Furthermore, A2 blocking improved hepatic perfusion during NR whereas A1 blocking reduced it.The study suggests that NR has a preconditioning effect by maintaining adequate adenosine and xanthine levels. During NR, adenosine protects the liver through A2 activation and damages it through A1 activation although simultaneous stimulation of both receptors exerts a clear beneficial effect. The possible relation of NR mechanism with other preconditioning mediators such as cAMP and nitric oxide synthesis are discussed.

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Section: Discussionmentioning

confidence: 99%

The Effect of Normothermic Recirculation is Mediated by Ischemic Preconditioning in NHBD Liver Transplantation

Net¹,

Valero²,

Almenara³

et al. 2005

American Journal of Transplantation

112

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“…Since cell metabolism decreases rapidly at temperatures below 37 "C, Schon et al, in an experimental study with ex-vivo perfused pig livers, postulate that normothermic oxygenated perfusion after extraction could offer the possibility of restoring metabolic processes and repairing damaged cells, thus resuscitating ischemically injured livers [47]. Some other experimental studies in liver and kidney transplantation have demonstrated the utility of normothermic recirculation to recover adenine nucleotide levels after warm ischemia [4,21,25,401, to increase reduced glutathione levels in both the liver and the kidney before cold storage [4] and to improve post transplant viability [19,25,511. In this way, Rijkmans et a1 state that it is possible to prevent irreversible damage through normothermic blood perfusion [43].…”

Section: Discussionmentioning

confidence: 99%

“…More recently, "normothermic recirculation" has been described in experimental studies [4,19,25,39,40,511. This method consist in the normothermic perfusion of the organs at 37 "C with hyperoxygenated blood by means of a cardiopulmonary bypass, before TBC.…”

Section: Introductionmentioning

confidence: 99%

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Normothermic recirculation reduces primary graft dysfunction of kidneys obtained from non-heart-beating donors

Valero¹,

Cabrer²,

Oppenheimer³

et al. 2000

Transplant Int

144

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Our aim was to analyze the short-and long-term function of kidneys procured from non-heartbeating donors (NHBD) by means of three techniques: in situ perfusion (ISP), total body cooling (TBC) and normothermic recirculation (NR). (52 Yo) showed delayed graft function (DGF) and 9 (16 %) showed primary non function (PNF). The actuarial graft survival rate was 76.4 Yo at 1 year and 56 YO at 5 years. The patient survival rate was 89.3 YO at 5 years. Incidence of DGF and PNF was significantly lower in kidneys perfused with NR than those with ISP or TBC ( P < 0.01). Duration of DGF was shorter in kidneys obtained through TBC than in kidneys obtained with ISP (P < 0.05).In conclusion, NR reduces the incidence of DGF and may be considered the method of choice for kidney procurement from NHBD.

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“…Warm ischemic damage in DCD can be reduced by lowering the temperature as quickly as possible or by perfusing the organs at body temperature to partly correct warm ischaemic injury, before the organs are cooled down .…”

Section: Procurement Of Dcd Kidneysmentioning

confidence: 99%

Recommendations for donation after circulatory death kidney transplantation in Europe

Heurn

Talbot

Nicholson³

et al. 2015

Transpl Int

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SUMMARY Donation after circulatory death (DCD) donors provides an invaluable source for kidneys for transplantation. Over the last decade, we have observed a substantial increase in the number of DCD kidneys, particularly within Europe. We provide an overview of risk factors associated with DCD kidney function and survival and formulate recommendations from the sixth international conference on organ donation in Paris, for bestpractice guidelines. A systematic review of the literature was performed using Ovid Medline, Embase and Cochrane databases. Topics are discussed, including donor selection, organ procurement, organ preservation, recipient selection and transplant management.

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Changes in adenine nucleotides and lipid hydroperoxides during normothermic cardiopulmonary bypass in a porcine model of type II non-heart-beating donor

Cited by 26 publications

References 7 publications

The Effect of Normothermic Recirculation is Mediated by Ischemic Preconditioning in NHBD Liver Transplantation

The Effect of Normothermic Recirculation is Mediated by Ischemic Preconditioning in NHBD Liver Transplantation

Normothermic recirculation reduces primary graft dysfunction of kidneys obtained from non-heart-beating donors

Recommendations for donation after circulatory death kidney transplantation in Europe

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