Changes in activities of superoxide dismutase, nitric oxide synthase, glutathione-dependent enzymes and the incidence of apoptosis in sheep corpus luteum during the estrous cycle

Al-Gubory, Kaïs Hussain; Ceballos-Picot, Irène; Nicole, Annie; Bolifraud, Philippe; Germain, Guy; Michaud, Marie; Mayeur, Camille; Blachier, François

doi:10.1016/j.bbagen.2005.06.018

Cited by 33 publications

(28 citation statements)

References 70 publications

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“…Gap junctions transfer regulatory molecules between adjacent cells and the response to chemical mediators in vitro is different between luteal slices and dissociated luteal cells [30]. Thus, NO may have different actions on the CL according to the culture system used, the estrous stage, or the species [5,8,[11][12][13][14]27]. However, overall findings suggest that although NO supports CL development and maintenance during the early luteal phase [14,24], it mediates luteolytic signals at the end of luteal phase [8,[12][13][14][15]31].…”

Section: Discussioncontrasting

confidence: 40%

“…Thus, NO may regulate genes responsible for induction of apoptosis directly or indirectly through cessation of P4 production and action in the CL. On the other hand, NO has also emerged as a potent inhibitor of apoptosis in luteal cells [5,27,[40][41][42]. It has been shown to NO inhibit caspase activity as a mechanism to block apoptosis [40].…”

Section: Discussionmentioning

confidence: 99%

“…However, it is not possible to apply this generalize those data to a wide range of animal species. Although NO has been shown to induce luteolysis in cattle [10,12,13,16,24,26], it plays luteotropic roles in different species, such as in the sheep CL [27]. Differences in NO action among species could be due to the methods used for examination and cell-tissue culture, differences in cytokines and ET-1 actions within bovine and ovine CL, and/or also differences in the ratio of PGE2 to PGF2α during the estrous cycle [10-14, 27, 28].…”

Section: Discussionmentioning

confidence: 99%

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Nitric Oxide Induces Apoptosis in Bovine Luteal Cells

Korzekwa

Okuda

Wocławek-Potocka

et al. 2006

Journal of Reproduction and Development

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Abstract. We previously showed in in vivo and in vitro studies that nitric oxide (NO) is engaged in luteolysis in cattle. Nitric oxide produced locally in the bovine corpus luteum (CL) inhibits progesterone (P4) synthesis and is suggested to be a component of the luteolytic cascade induced by uterine prostaglandin (PG) F2α. In the present study, the molecular mechanisms of NO action during structural luteolysis were studied in cultured bovine luteal cells (Days 15-17 of the estrous cycle). The effects of the NO donor (NONOate; 10 -4 M) on DNA fragmentation, cell viability, P4 production and caspase-3 activity were compared with those of PGF2α (10 -6 M). Moreover, mobilization of intracellular calcium [Ca 2+ ]i and gene expressions of Fas-L, Fas, bcl-2, bax, and caspase-3 in the cells were determined by semi-quantitative RT-PCR after NONOate treatment. Caspase-3 activity was examined calorimetrically. Contrary to PGF2α NONOate decreased cell viability. DNA fragmentation after NONOate treatment increased by more than with PGF22α. NONOate increased mobilization of [Ca 2+ ]i in the cells. Although the NO donor did not affect Fas-L and bcl-2 gene expression, it stimulated Fas and bax mRNA and caspase-3 expression. The ratio of bcl-2 to bax mRNA level decreased in the cells treated with NONOate. Moreover, NONOate stimulated caspase-3 activity more effectively than PGF2α. The overall results suggest that NO is a luteolytic factor that plays a crucial role in regulation of the estrous cycle in structural luteolysis by inducing apoptosis of luteal cells in cattle.

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Section: Discussioncontrasting

confidence: 40%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Nitric Oxide Induces Apoptosis in Bovine Luteal Cells

Korzekwa

Okuda

Wocławek-Potocka

et al. 2006

Journal of Reproduction and Development

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“…Determining the profile of angiogenic factors responsible for vascular changes may prove to be important in determining how the composition of the CL is altered during regression. While there are several factors which have been shown to influence vascularity of different tissues, some of the primary regulators of ovarian angiogenesis and vascular function include: the vascular endothelial growth factor (VEGF) system (including the VEGF receptors and the neuropilins; Ferrara et al 1992, Shalaby et al 1995, Neufeld et al 1999, Stacker & Achen 1999, fibroblastic growth factors (FGF) and receptors (Klagsbrun & D'Amore 1991, Neuvians et al 2004a, the angiopoietins (ANGPT) and their receptor Tie-2 (Sato et al 1993, Davis & Yancopoulos 1999, Hazzard et al 2000, Wulff et al 2000, Tanaka et al 2004, the nitric oxide (NO) system (Motta et al 2001, Klipper et al 2004, Shi et al 2004, Al-Gubory et al 2005, and the renin-angiotensin system (Hansel & Blair 1996, Hayashi & Miyamoto 1999, Kobayashi et al 2001. While it is most certain that there are several other angiogenic factors playing a role in the regulation of vascular function, selected members from these families were investigated in the current experiment.…”

Section: Introductionmentioning

confidence: 99%

Vascular composition, apoptosis, and expression of angiogenic factors in the corpus luteum during prostaglandin F2α-induced regression in sheep

Vonnahme¹,

Redmer²,

Borowczyk³

et al. 2006

Reproduction

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Corpora lutea and blood samples were collected from superovulated ewes 0, 4, 8, 12 and 24 h after prostaglandin F 2a (PGF) analog injection on day 10 of the estrous cycle. Changes in vascular cell and fibroblast composition, apoptosis and mRNA expression for several angiogenic factors in the corpus luteum (CL) were determined. While peripheral progesterone concentration decreased at 24 h after PGF injection, CL weight did not change. The area of positive BS-1 lectin staining (endothelial cell marker), smooth muscle cell actin (SMCA; pericyte and SMC marker), collagen type 1 (fibroblast marker), and the rate of cell death changed in luteal tissues after PGF treatment. In association with these cellular changes, mRNA for several angiogenic factors including vascular endothelial growth factor (VEGF) and receptors (Flt and KDR), basic fibroblast growth factor (FGF2) and receptor, angiopoietin (ANGPT) 1 and receptor Tie-2, endothelial nitric oxide synthase (NOS3), and angiotensin II receptor 1 (AT1) were altered. Changes in endothelial cell marker expression were positively correlated with changes in VEGF and NO systems. In addition, changes in mRNA expression for VEGF, Flt and KDR were positively correlated with changes in ANGPT2, Tie-2, and NOS3, indicating a functional relationship. This data demonstrates that after an initial increase, the endothelial component of the vascular bed decreases during PGF-induced luteal regression. However, SMCA expression remained high during luteal regression, potentially indicating a role of pericytes and vascular SMC in luteolysis, likely to regulate tissue remodeling and to maintain the integrity of larger blood vessels. Further, it appears that early regression may increase collagen type 1 production and/or expression by fibroblasts. Expression of angiogenic factors is influenced by PGF-induced luteolysis and may serve to maintain vascular structure in order to aid luteal regression.

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“…Protein concentrations were determined by Lowry's method (Lowry et al 1951). Enzyme activities of SOD1, SOD2, CAT and GPX in the supernatant were determined as described previously in detail (Al-Gubory et al 2005, Garrel et al 2007. Total SOD activity was measured using the pyrogallol assay based on the competition between pyrogallol oxidation by superoxide radicals and superoxide dismutation by SOD (Marklund & Marklund 1974).…”

Section: Antioxidant Enzyme Activity Assaysmentioning

confidence: 99%

Antioxidant adaptive responses of extraembryonic tissues from cloned and non-cloned bovine conceptuses to oxidative stress during early pregnancy

Al-Gubory¹,

Garrel²,

Delatouche³

et al. 2010

Reproduction

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Placental oxidative stress has been suggested as a key factor in early pregnancy failure. Abnormal placental development limits success in pregnancies obtained by somatic cell nuclear transfer (SCNT). Malondialdehyde (MDA) content, an index of oxidative stress, and superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) activities were determined in bovine extraembryonic tissues of SCNT or artificial insemination (AI) conceptuses. Chorionic tissues of SCNT and AI conceptuses show no difference in MDA content at day 32 of pregnancy. MDA content in chorionic tissues of SCNT and AI conceptuses decreased from day 32 to 62 of pregnancy. MDA content was lower in chorionic tissues of SCNT conceptuses than that in chorionic tissues of AI conceptuses at day 62 of pregnancy. SOD1, SOD2 and GPX activities in chorionic tissues of SCNT conceptuses were not different from those in chorionic tissues of AI conceptuses at both gestational ages. CAT activity in chorionic tissues of SCNT conceptuses was lower at day 32, and it was higher at day 62 of pregnancy than that in chorionic tissues of AI conceptuses. CAT and GPX activities increased in chorionic tissues of SCNT conceptuses with gestational age. SOD1 activity decreased while that of SOD2 and GPX increased in chorionic tissues of AI conceptuses with gestational age. At day 62 of pregnancy, MDA content and enzyme activities in cotyledonary tissues were not different between AI and SCNT conceptuses. Different antioxidant mechanisms may operate within the chorion of AI and SCNT conceptuses. Further experiments are required to elucidate this point.

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Changes in activities of superoxide dismutase, nitric oxide synthase, glutathione-dependent enzymes and the incidence of apoptosis in sheep corpus luteum during the estrous cycle

Cited by 33 publications

References 70 publications

Nitric Oxide Induces Apoptosis in Bovine Luteal Cells

Nitric Oxide Induces Apoptosis in Bovine Luteal Cells

Vascular composition, apoptosis, and expression of angiogenic factors in the corpus luteum during prostaglandin F2α-induced regression in sheep

Antioxidant adaptive responses of extraembryonic tissues from cloned and non-cloned bovine conceptuses to oxidative stress during early pregnancy

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