Changes in Activated T Cells in the Blood Correlate With Disease Activity in Multiple Sclerosis

Abstract

There is a linkage between peripheral T-lymphocyte activation as measured by cell surface markers and disease activity in patients with multiple sclerosis. Arch Neurol. 2000;57:1183-1189

Expression of ectonucleotidase CD39 by Foxp3+ Treg cells: hydrolysis of extracellular ATP and immune suppression

“…In particular, patients affected by MS show a significant decrease in the suppressive capacity of CD4 ϩ CD25 high cells, 31 although most studies did not reveal any significant changes in their total number. 31,36,37 In line with these reports, we observed only a slight reduction of the fraction of CD4 ϩ CD25 high T cells in the blood of patients affected by the remitting/relapsing form of MS ( Figure 7C). The striking fluctuations in the amount of CD39 expressing Treg cells among individuals, however, suggested that CD39 in combination with CD25 may be a more appropriate marker than CD25 alone.…”

Expression of ectonucleotidase CD39 by Foxp3+ Treg cells: hydrolysis of extracellular ATP and immune suppression

“…In particular, patients affected by MS show a significant decrease in the suppressive capacity of CD4 ϩ CD25 high cells, 31 although most studies did not reveal any significant changes in their total number. 31,36,37 In line with these reports, we observed only a slight reduction of the fraction of CD4 ϩ CD25 high T cells in the blood of patients affected by the remitting/relapsing form of MS ( Figure 7C). The striking fluctuations in the amount of CD39 expressing Treg cells among individuals, however, suggested that CD39 in combination with CD25 may be a more appropriate marker than CD25 alone.…”

Normal bone marrow hematopoietic stem cell reserves and normal stromal cell function support the use of autologous stem cell transplantation in patients with multiple sclerosis

“…It has been previously reported that the presence of activated T cells in the BM may affect the growth and survival characteristics of BM hematopoietic progenitor cells or even the hematopoiesis supporting capacity of BM stromal cells by inducing intricate cell-tocell interactions and proinflammatory cytokine production. 26,[29][30][31][32][33][34][35] In agreement with previous reports, we found increased numbers of activated T cells in PB of our MS patients, 15,[36][37][38] and also increased proportions of activated T cells in patients' BM. Interestingly, the proportion of HLA-DR þ and CD38 þ cells in patient BM CD3 þ cells inversely correlated with the number of CFCs in the BMMC fraction, suggesting that activated T cells may be implicated in the defective clonogenic potential of hematopoietic progenitor cells in MS.…”

Modulation of the central memory and Tr1-like regulatory T cells in multiple sclerosis patients responsive to interferon-beta therapy

“…25 RTEs were defined as naive CD4 + cells expressing the CD31 molecule (Figure 1B); 26 Tregs were CD4 + CD25 + CD127 low/- lymphocytes (Figure 1C) 27 and RTE-Tregs were RTEs with the phenotypic characteristics of Tregs (Figure 1E). 14 As clearly shown in the representative example, and accordingly to Venken et al, 28 RTE-Tregs (Figure 1E) did not contain the cells with high expression of CD25 that are present in the total Treg population (Figure 1C). Then, we phenotyped the CD4 + CD25 + CD127 low/- Treg population for CD45RA and CCR7.…”

Expression of ectonucleotidase CD39 by Foxp3+ Treg cells: hydrolysis of extracellular ATP and immune suppression

“…In particular, patients affected by MS show a significant decrease in the suppressive capacity of CD4 ϩ CD25 high cells, 31 although most studies did not reveal any significant changes in their total number. 31,36,37 In line with these reports, we observed only a slight reduction of the fraction of CD4 ϩ CD25 high T cells in the blood of patients affected by the remitting/relapsing form of MS ( Figure 7C). The striking fluctuations in the amount of CD39 expressing Treg cells among individuals, however, suggested that CD39 in combination with CD25 may be a more appropriate marker than CD25 alone.…”

Normal bone marrow hematopoietic stem cell reserves and normal stromal cell function support the use of autologous stem cell transplantation in patients with multiple sclerosis

“…It has been previously reported that the presence of activated T cells in the BM may affect the growth and survival characteristics of BM hematopoietic progenitor cells or even the hematopoiesis supporting capacity of BM stromal cells by inducing intricate cell-tocell interactions and proinflammatory cytokine production. 26,[29][30][31][32][33][34][35] In agreement with previous reports, we found increased numbers of activated T cells in PB of our MS patients, 15,[36][37][38] and also increased proportions of activated T cells in patients' BM. Interestingly, the proportion of HLA-DR þ and CD38 þ cells in patient BM CD3 þ cells inversely correlated with the number of CFCs in the BMMC fraction, suggesting that activated T cells may be implicated in the defective clonogenic potential of hematopoietic progenitor cells in MS.…”

Modulation of the central memory and Tr1-like regulatory T cells in multiple sclerosis patients responsive to interferon-beta therapy

“…25 RTEs were defined as naive CD4 + cells expressing the CD31 molecule (Figure 1B); 26 Tregs were CD4 + CD25 + CD127 low/- lymphocytes (Figure 1C) 27 and RTE-Tregs were RTEs with the phenotypic characteristics of Tregs (Figure 1E). 14 As clearly shown in the representative example, and accordingly to Venken et al, 28 RTE-Tregs (Figure 1E) did not contain the cells with high expression of CD25 that are present in the total Treg population (Figure 1C). Then, we phenotyped the CD4 + CD25 + CD127 low/- Treg population for CD45RA and CCR7.…”

Expression of ectonucleotidase CD39 by Foxp3+ Treg cells: hydrolysis of extracellular ATP and immune suppression

“…In particular, patients affected by MS show a significant decrease in the suppressive capacity of CD4 ϩ CD25 high cells, 31 although most studies did not reveal any significant changes in their total number. 31,36,37 In line with these reports, we observed only a slight reduction of the fraction of CD4 ϩ CD25 high T cells in the blood of patients affected by the remitting/relapsing form of MS ( Figure 7C). The striking fluctuations in the amount of CD39 expressing Treg cells among individuals, however, suggested that CD39 in combination with CD25 may be a more appropriate marker than CD25 alone.…”

Normal bone marrow hematopoietic stem cell reserves and normal stromal cell function support the use of autologous stem cell transplantation in patients with multiple sclerosis

“…It has been previously reported that the presence of activated T cells in the BM may affect the growth and survival characteristics of BM hematopoietic progenitor cells or even the hematopoiesis supporting capacity of BM stromal cells by inducing intricate cell-tocell interactions and proinflammatory cytokine production. 26,[29][30][31][32][33][34][35] In agreement with previous reports, we found increased numbers of activated T cells in PB of our MS patients, 15,[36][37][38] and also increased proportions of activated T cells in patients' BM. Interestingly, the proportion of HLA-DR þ and CD38 þ cells in patient BM CD3 þ cells inversely correlated with the number of CFCs in the BMMC fraction, suggesting that activated T cells may be implicated in the defective clonogenic potential of hematopoietic progenitor cells in MS.…”

Modulation of the central memory and Tr1-like regulatory T cells in multiple sclerosis patients responsive to interferon-beta therapy

“…25 RTEs were defined as naive CD4 + cells expressing the CD31 molecule (Figure 1B); 26 Tregs were CD4 + CD25 + CD127 low/- lymphocytes (Figure 1C) 27 and RTE-Tregs were RTEs with the phenotypic characteristics of Tregs (Figure 1E). 14 As clearly shown in the representative example, and accordingly to Venken et al, 28 RTE-Tregs (Figure 1E) did not contain the cells with high expression of CD25 that are present in the total Treg population (Figure 1C). Then, we phenotyped the CD4 + CD25 + CD127 low/- Treg population for CD45RA and CCR7.…”