Changes in acetylation of lysine 5 on histone H4 in canine oocytes following in vitro maturation

Motheo, T. F.; Arnold, Daniel; Padilha-Nakaghi, Luciana Cristina; Pires-Buttler, E. A.; Alves, A. E.; Apparício, M.; Vicente, W. R. R.; Lopes, Flávia L.

doi:10.1111/rda.12897

Reprod Domestic Animals

2016

DOI: 10.1111/rda.12897

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Changes in acetylation of lysine 5 on histone H4 in canine oocytes following in vitro maturation

T. F. Motheo

Daniel Arnold

Luciana Cristina Padilha-Nakaghi

et al.

Abstract: ContentsPost-translational modifications of histones, such as acetylation, are involved in regu- remains to be investigated whether these changes occur during in vivo maturation.

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Cited by 2 publications

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Dynamic changes of histone acetylation and methylation in bovine oocytes, zygotes, and preimplantation embryos

Wang

et al. 2020

J Exp Zool Pt B

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Histone modifications play important roles in regulating chromatin dynamic changes. In this study, acetylated histone H3 lysine 9 and 18 (H3K9ac and H3K18ac), acetylated histone H4 lysine 5 and 8 (H4K5ac and H4K8ac), tri‐methylation histone H3 lysine 4 (H3K4me3), di‐methylation histone H3 lysine 9 (H3K9me2) are investigated in bovine oocytes, zygote, and preimplantation. During meiosis, H3K9ac and H3K18ac are erased after germinal vesicle breakdown, H4K8ac is erased after metaphase I (MI). Although H4K5ac is erased at MI, it is redetectable after this stage. However, histone methylations have no significant change during meiosis. During fertilization, intensive H4K5ac and H4K8ac are resumed on male and female chromatins at postfertilization 4 and 8 hr, respectively. H3K9ac and H3K18ac are resumed on both male and female chromatins at postfertilization 8 and 12 hr, respectively. H3K4me3 and H3K9me2 gradually increased on male chromatin after postfertilization 8 hr, while these two signals on female chromatin are detectable from postfertilization 2–18 hr. During embryo cleavage, H3K9ac, H3K18ac, and H3K4me3 are reduced at 8‐cell stage, and then start to increase. H4K5ac, H4K8ac, and H3K9me2 increase after the 4‐cell stage. At interphase, H4K5ac and H4K8ac are more intensive in nuclear periphery from 2‐ to 8‐cell stages. During mitosis, the signal of H4K8ac is intensive at chromosome periphery. In summary, during both oocyte meiosis and fertilization, the dynamic changes of both histone acetylations and methylations happen in a process of lysine residue‐specific and species‐specific. During preimplantation development, the dynamic patterns of both H3K9ac and H3K18ac are similar to that of H3K4me3, while the dynamic pattern of H4K5ac is similar to that of H4K8ac. These results will be helpful for understanding the effect of histone posttranslational modifications on bovine reproduction and development.

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Dynamic changes of histone acetylation and methylation in bovine oocytes, zygotes, and preimplantation embryos

Wang

et al. 2020

J Exp Zool Pt B

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HASPIN kinase mediates histone deacetylation to regulate oocyte meiotic maturation in pigs

Cao

et al. 2019

Reproduction

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HASPIN kinase-catalyzed phosphorylation of histone H3 on threonine 3 (H3T3p) directs the activity and localization of chromosomal passenger complex (CPC) and spindle assembly checkpoint (SAC) to regulate chromosome condensation and segregation in both mitosis and meiosis. However, the function of HASPIN kinase in the meiotic maturation of porcine oocytes is not yet known. Here, we found that HASPIN mRNA is constantly expressed in porcine oocyte maturation and subsequent early embryo development. H3T3p is highly enriched on chromosomes at germinal vesicle breakdown (GVBD) stage and thereafter maintains a low level in progression through metaphase I (MI) to metaphase II (MII). Correspondingly, H3T3p was completely abolished in oocytes treated with an inhibitor of HASPIN kinase. Functionally, inhibition of HASPIN activity led to a significant reduction in the rate of oocyte meiotic maturation and the limited cumulus expansion. Additionally, HASPIN inhibition caused both spindle disorganization and chromosome misalignment in oocytes at MI and MII stage. Importantly, HASPIN inhibition severely prevented deacetylation of several highly conserved lysine (K) residues of histone H3 and H4 including H3K9, H3K14, H4K5, H4K8, H4K12 and H4K16 on the metaphase chromosomes during oocyte meiotic maturation. Taken together, these results demonstrate that HASPIN kinase regulates porcine oocyte meiotic maturation via modulating histone deacetylation.

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Changes in acetylation of lysine 5 on histone H4 in canine oocytes following in vitro maturation

Abstract: ContentsPost-translational modifications of histones, such as acetylation, are involved in regu- remains to be investigated whether these changes occur during in vivo maturation.

Cited by 2 publications

References 41 publications

Dynamic changes of histone acetylation and methylation in bovine oocytes, zygotes, and preimplantation embryos

Dynamic changes of histone acetylation and methylation in bovine oocytes, zygotes, and preimplantation embryos

HASPIN kinase mediates histone deacetylation to regulate oocyte meiotic maturation in pigs

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