Changes in abundance and composition of nitrifying communities in barley (Hordeum vulgare L.) rhizosphere and bulk soils over the growth period following combined biochar and urea amendment

Li, Yu; Homyak, Peter M.; Kang, Xiaoxi; Brookes, Philip C.; Ye, Yikai; Lin, Yeneng; Afzal, Muhammad; Xu, Jianming

doi:10.1007/s00374-019-01410-6

Cited by 23 publications

(11 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To better understand the complex interaction of factors, it is necessary to explore how soil chemical properties influenced nitrite oxidizers community structures under biochar and/or NPK addition. The RDA analysis suggested that the variability of nitrite oxidizers was remarkably affected by soil pH and multiple nutrients (Figure 4), in agreement with recent studies (Han et al, 2018;Wang et al, 2019a;Yu et al, 2019). Wang et al (2019a) found that pH, OM, and available N are key factors affecting Comammox community structure.…”

Section: Effects Of Biochar And/or Npk Amendment On Potential Nitrification Rate In Rhizosphere Soilsupporting

confidence: 89%

“…Effects of Biochar and/or NPK Amendment on Abundances and Community Structures of Canonical Ammonia Oxidizers in Rhizosphere Soil Canonical ammonia oxidizers (AOA and AOB) carry out the first step of nitrification, in which ammonia is converted to nitrite by encoding the subunit of ammonia monooxygenase, the limiting factor in soil nitrification (Shi et al, 2019). In agreement with previous studies (Leininger et al, 2006;He et al, 2018;Yu et al, 2019), AOA dominated in abundance compared to AOB in the tested soil. Single biochar amendment had little impact on AOA abundance, which was contrary to a previous study (Xiao et al, 2019).…”

Section: Effects Of Biochar And/or Npk Amendment On Potential Nitrification Rate In Rhizosphere Soilsupporting

confidence: 89%

“…Previous studies found that Nitrobacter-like NOB was more responsive than Nitrospira-like NOB to high urea additions (Ke et al, 2013;Han et al, 2018). In addition, the rhizosphere and associated root exudates could be the hotspots of nutrient cycling in agricultural soil (Liu et al, 2018;Yu et al, 2019), but there is limited information available regarding the impacts of N fertilizer on nitrification and related nitrifiers in rhizosphere soil.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Ammonia- and Nitrite-Oxidizing Bacteria are Dominant in Nitrification of Maize Rhizosphere Soil Following Combined Application of Biochar and Chemical Fertilizer

et al. 2021

View full text Add to dashboard Cite

Autotrophic nitrification is regulated by canonical ammonia-oxidizing archaea (AOA) and bacteria (AOB) and nitrite-oxidizing bacteria (NOB). To date, most studies have focused on the role of canonical ammonia oxidizers in nitrification while neglecting the NOB. In order to understand the impacts of combined biochar and chemical fertilizer addition on nitrification and associated nitrifiers in plant rhizosphere soil, we collected rhizosphere soil from a maize field under four different treatments: no fertilization (CK), biochar (B), chemical nitrogen (N) + phosphorus (P) + potassium (K) fertilizers (NPK), and biochar + NPK fertilizers (B + NPK). The potential nitrification rate (PNR), community abundances, and structures of AOA, AOB, complete ammonia-oxidizing bacteria (Comammox Nitrospira clade A), and Nitrobacter- and Nitrospira-like NOB were measured. Biochar and/or NPK additions increased soil pH and nutrient contents in rhizosphere soil. B, NPK, and B + NPK treatments significantly stimulated PNR and abundances of AOB, Comammox, and Nitrobacter- and Nitrospira-like NOB, with the highest values observed in the B + NPK treatment. Pearson correlation and random forest analyses predicted more importance of AOB, Comammox Nitrospira clade A, and Nitrobacter- and Nitrospira-like NOB abundances over AOA on PNR. Biochar and/or NPK additions strongly altered whole nitrifying community structures. Redundancy analysis (RDA) showed that nitrifying community structures were significantly affected by pH and nutrient contents. This research shows that combined application of biochar and NPK fertilizer has a positive effect on improving soil nitrification by affecting communities of AOB and NOB in rhizosphere soil. These new revelations, especially as they related to understudied NOB, can be used to increase efficiency of agricultural land and resource management.

show abstract

Section: Effects Of Biochar And/or Npk Amendment On Potential Nitrification Rate In Rhizosphere Soilsupporting

confidence: 89%

Section: Effects Of Biochar And/or Npk Amendment On Potential Nitrification Rate In Rhizosphere Soilsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ammonia- and Nitrite-Oxidizing Bacteria are Dominant in Nitrification of Maize Rhizosphere Soil Following Combined Application of Biochar and Chemical Fertilizer

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The concentration of AP was also significantly increased with the amendment of biochar and BCF. This suggested that the sorption of N and P by biochar could be a crucial process ( Yu et al, 2020 ). In other studies, these increases were regulated by the available nutrients of biochar and interaction of biochar with fertilizer ( Ibrahim et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Different Responses of Soil Bacterial and Fungal Communities to 3 Years of Biochar Amendment in an Alkaline Soybean Soil

Gao

Guo

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

Biochar as a soil amendment has been regarded as a promising way to improve soil fertility. However, the response of microbial community after biochar and biochar compound fertilizer (BCF) application has not been thoroughly elucidated. This study evaluated the changes in abundance and composition of bacterial and fungal communities using quantitative real-time PCR (qPCR) and Illumina MiSeq amplicon sequencing. The field experiment ran for 3 years and comprised five treatments: chemical fertilizer as control (CK), straw-returning combined with chemical fertilizer (CS), low biochar application combined with chemical fertilizer (LB), high biochar application combined with chemical fertilizer (HB) and BCF. The results showed that biochar amendment results no changes in the abundance and diversity of bacteria in the bulk and rhizosphere soils. However, the abundance of soil fungi was significantly increased by biochar amendment (LB and HB). LB treatment significantly increased the fungal alpha diversity, while there was no significant change under HB. Furthermore, the dominant bacterial phyla found in the samples were Proteobacteria, Actinobacteria, and Acidobacteria. Biochar addition increased the relative abundance of Actinobacteria in both bulk and rhizosphere soils. The dominant fungal phyla were Ascomycota, Mortierellomycota, and Basidiomycota. The relative abundance of Ascomycota significantly decreased, but Mortierellomycota significantly increased in LB and HB. In addition, redundancy analysis indicated that the changes in bacterial and fungal communities are associated with soil properties such as SOC and TN, which are crucial contributors in regulating the community composition. This study is expected to provide significant theoretical and practical knowledge for the application of biochar in agricultural ecosystem.

show abstract

“…The pH of soil (1:2.5 w/v) and biochar (1:20 w/v) were measured in deionized water (Yang et al, 2019). The elemental composition of soil and biochars was determined with a CNS analyzer (CNS, 2000; LECO, USA; Yu et al, 2020a). Dissolved organic C (DOC) and N (DON) concentrations were determined in water extracts (soil: 1:5 w/w; biochar: 1:20 w/w; passed through 0.45-μm filters) via a TOC analyzer (Multi N/C 3100 HT1300, Analytik Jena AG, Germany) (Dai et al, 2014).…”

Section: N 2 O and Co 2 Measurements And Chemical Analysesmentioning

confidence: 99%

Bacterial community structure and putative nitrogen-cycling functional traits along a charosphere gradient under waterlogged conditions

Huang

et al. 2021

Soil Biology and Biochemistry

Self Cite

View full text Add to dashboard Cite

Unique spatial niches created by biochar form a dynamic biogeochemical soil zone, termed the "charosphere". Charosphere soil has different properties from either soil or biochar, often acting as a hotspot for microbial activity. However, the direction and magnitude of charosphere effects on soil processes and microbial communities remain inconclusive. Herein, we designed a multi-sectional box to separate charosphere soils from biochar under waterlogged conditions, investigating millimeter-scale changes in bacterial communities and distributions of specific genera across the charosphere created by pristine biochar (produced from corn stover at 300 • C), acid-modified biochar and washed biochar. The pristine biochar did not increase bacterial α-diversity but altered bacterial composition by promoting the growth of specific bacterial taxa and suppressing other species. In comparison, washed and acid-modified biochar increased the bacterial α-diversity. The pristine biochar significantly decreased N 2 O emission and NO 3 − concentration owing to a lower soil nitrification potential. Abundances of N-cycling functional taxa were higher in the near-charosphere than outer-charosphere zone, especially for Bacillus (N 2 -fixation), MND1 (ammonia oxidation) and Rhodopseudomonas (denitrification). The spatial distribution of functional genes and genera documents the importance of heterogeneity within the charosphere gradient, providing a novel perspective into functional geometry to understand how biochar reduces N losses from agroecosystems.

show abstract

Changes in abundance and composition of nitrifying communities in barley (Hordeum vulgare L.) rhizosphere and bulk soils over the growth period following combined biochar and urea amendment

Cited by 23 publications

References 68 publications

Ammonia- and Nitrite-Oxidizing Bacteria are Dominant in Nitrification of Maize Rhizosphere Soil Following Combined Application of Biochar and Chemical Fertilizer

Ammonia- and Nitrite-Oxidizing Bacteria are Dominant in Nitrification of Maize Rhizosphere Soil Following Combined Application of Biochar and Chemical Fertilizer

Different Responses of Soil Bacterial and Fungal Communities to 3 Years of Biochar Amendment in an Alkaline Soybean Soil

Bacterial community structure and putative nitrogen-cycling functional traits along a charosphere gradient under waterlogged conditions

Contact Info

Product

Resources

About