Changes in Abscisic Acid Biosynthesis and Catabolism during Dormancy Breaking in Fagus sylvatica Embryo

Page-Degivry, M.Th. Le; Garello, G.; Barthe, Philippe

doi:10.1007/pl00006978

Cited by 40 publications

(22 citation statements)

References 15 publications

(14 reference statements)

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“…However, when chilled seeds were returned to standard germination conditions we then observed a new and strong decrease in the ABA level to 70 pmol g À1 DW, just before chilled seeds germinated. These results were in accordance with previous observations of Le Page-Degivry et al (1997), which established that the main effect of chilling on Fagus sylvatica seeds was a strong reduction in their ABA content following their transfer from chilling conditions to standard conditions for germination. D seeds sown on nitrate presented an accelerated catabolism of ABA during the 2 days following sowing, in comparison with control D seeds.…”

Section: Release From Dormancysupporting

confidence: 95%

“…The predominant pathway by which ABA is catabolized in plants is through oxidative degradation to 8¢-hydroxy-ABA with subsequent conversion to phaseic acid then reduction to dihydrophaseic acid (Walton and Li 1995). This pathway operates in the seeds of various species, for instance Fraxinus americana (Sondheimer et al 1974), Fagus sylvatica (Le Page-Degivry et al 1997) and barley (Jacobsen et al 2002), where it has been shown to be more intense in ND seeds versus D seeds. The same mechanism could operate in Cvi seeds.…”

Section: Release From Dormancymentioning

confidence: 97%

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Changes in endogenous abscisic acid levels during dormancy release and maintenance of mature seeds: studies with the Cape Verde Islands ecotype, the dormant model of Arabidopsis thaliana

Ali-Rachedi¹,

Bouinot²,

Wagner

et al. 2004

Planta

353

303

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Mature seeds of the Cape Verde Islands (Cvi) ecotype of Arabidopsis thaliana (L.) Heynh. show a very marked dormancy. Dormant (D) seeds completely fail to germinate in conditions that are favourable for germination whereas non-dormant (ND) seeds germinate easily. Cvi seed dormancy is alleviated by after-ripening, stratification, and also by nitrate or fluridone treatment. Addition of gibberellins to D seeds does not suppress dormancy efficiently, suggesting that gibberellins are not directly involved in the breaking of dormancy. Dormancy expression of Cvi seeds is strongly dependent on temperature: D seeds do not germinate at warm temperatures (20-27 degrees C) but do so easily at a low temperature (13 degrees C) or when a fluridone treatment is given to D seeds sown at high temperature. To investigate the role of abscisic acid (ABA) in dormancy release and maintenance, we measured the ABA content in both ND and D seeds imbibed using various dormancy-breaking conditions. It was found that dry D seeds contained higher amounts of ABA than dry ND after-ripened seeds. During early imbibition in standard conditions, there was a decrease in ABA content in both seeds, the rate of which was slower in D seeds. Three days after sowing, the ABA content in D seeds increased specifically and then remained at a high level. When imbibed with fluridone, nitrate or stratified, the ABA content of D seeds decreased and reached a level very near to that of ND seeds. In contrast, gibberellic acid (GA3) treatment caused a transient increase in ABA content. When D seeds were sown at low optimal temperature their ABA content also decreased to the level observed in ND seeds. The present study indicates that Cvi D and ND seeds can be easily distinguished by their ability to synthesize ABA following imbibition. Treatments used here to break dormancy reduced the ABA level in imbibed D seeds to the level observed in ND seeds, with the exception of GA3 treatment, which was active in promoting germination only when ABA synthesis was inhibited.

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Section: Release From Dormancysupporting

confidence: 95%

Section: Release From Dormancymentioning

confidence: 97%

Changes in endogenous abscisic acid levels during dormancy release and maintenance of mature seeds: studies with the Cape Verde Islands ecotype, the dormant model of Arabidopsis thaliana

Ali-Rachedi¹,

Bouinot²,

Wagner

et al. 2004

Planta

353

303

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“…Moreover, ABA is necessary for dormancy maintenance during postimbibition (Le Page-Degivry and Garello 1992; Wang et al 1995;Bianco et al 1997;Le Page-Degivry et al 1997;Yoshioka et al 1998). For instance, when tobacco (Nicotiana plumbaginifolia) seeds are imbibed, there is an accumulation of ABA in dormant seeds but not in seeds that have been allowed to after-ripen.…”

Section: Introductionmentioning

confidence: 96%

“…germinate). For example, endogenous ABA levels decline in beechnut (Fagus sylvatica) embryos to the same extent regardless of dormancy status, yet [ 3 H]ABA turnover is greater in embryos subjected to a dormancy-breaking cold pretreatment than in embryos that remain dormant because of their maintenance at 23°C (Le Page-Degivry et al 1997). Increased ABA catabolism is associated with dormancy termination of seeds of yellow-cedar (Chamaecyparis nootkatensis), beechnut, Douglas fir (Pseudotsuga menziesii), and barley (Hordeum vulgare) (Le Page-Degivry et al 1997;Schmitz et al 2000Schmitz et al , 2002Corbineau et al 2002;Jacobsen et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Dormancy termination of western white pine ( Pinus monticola Dougl. Ex D. Don) seeds is associated with changes in abscisic acid metabolism

Feurtado

Ambrose

Cutler

et al. 2004

Planta

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Western white pine (Pinus monticola) seeds exhibit deep dormancy at maturity and seed populations require several months of moist chilling to reach their uppermost germination capacities. Abscisic acid (ABA) and its metabolites, phaseic acid (PA), dihydrophaseic acid (DPA), 7'-hydroxy ABA (7'OH ABA) and ABA-glucose ester (ABA-GE), were quantified in western white pine seeds during dormancy breakage (moist chilling) and germination using an HPLC-tandem mass spectrometry method with multiple reaction monitoring and internal standards incorporating deuterium-labeled analogs. In the seed coat, ABA and metabolite levels were high in dry seeds, but declined precipitously during the pre-moist-chilling water soak to relatively low levels thereafter. In the embryo and megagametophyte, ABA levels decreased significantly during moist chilling, coincident with an increase in the germination capacity of seeds. ABA catabolism occurred via several routes, depending on the stage and the seed tissue. Moist chilling of seeds led to increases in PA and DPA levels in both the embryo and megagametophyte. Within the embryo, 7'OH ABA and ABA-GE also accumulated during moist chilling; however, 7'OH ABA peaked early in germination. Changes in ABA flux, i.e. shifts in the ratio between biosynthesis and catabolism, occurred at three distinct stages during the transition from dormant seed to seedling. During moist chilling, the relative rate of ABA catabolism exceeded ABA biosynthesis. This trend became even more pronounced during germination, and germination was also accompanied by a decrease in the ABA catabolites DPA and PA, presumably as a result of their further metabolism and/or leaching/transport. The transition from germination to post-germinative growth was accompanied by a shift toward ABA biosynthesis. Dormant imbibed seeds, kept in warm moist conditions for 30 days (after an initial 13 days of soaking), maintained high ABA levels, while the amounts of PA, 7'OH ABA, and DPA decreased or remained at steady-state levels. Thus, in the absence of conditions required to break dormancy there were no net changes in ABA biosynthesis and catabolism.

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“…According to our results ABA level decreased continually in cotyledons as well as in embryonic axes during stratification and the drop of more than 50% is linked with the successful breaking the dormancy. In beech seeds the decrease in the level of ABA correlated with ABA degradation during the dormancy breaking and with the inability to synthesize ABA (Le Page-Degivry et al 1997). High ABA level in embryos inhibits the transition to germination (Rodríguez-Gacio et al 2009).…”

Section: Discussionmentioning

confidence: 99%

Storage compounds, ABA and fumarase in Fagus sylvatica embryos during stratification

Eliášová¹,

Pešek²,

Vondráková³

2015

Dendrobiology

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Understanding the mechanisms of seed dormancy is essential for planning optimal seed storage and for breaking the dormancy of stored seeds. Our experiments focused on three aspects of characterization of breaking the dormancy of Fagus sylvatica seeds during moist low temperature stratification. 1) We used the changes of ABA content in embryos during the whole process of stratification as the marker of the depth of dormancy: the decrease in the depth of dormancy (according standard germination tests) was directly proportional to the drop in endogenous ABA content in both cotyledons and embryonic axes. 2) Fumarase activity (the second marker of dormancy) increased gradually during stratification.3) The histochemical visualisation of storage compounds as the third marker of dormancy documented the changes in storage protein deposition during stratification. The proteinaceous content of storage vacuoles disappeared; the vacuoles diminished, petered out or fused, creating central vacuoles. This process commenced with imbibition of the seeds and it was more pronounced in the cells in the external parts of the cotyledons. No changes in the size and location of starch grains or calcium oxalate crystals linked with stratification were detected.

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Changes in Abscisic Acid Biosynthesis and Catabolism during Dormancy Breaking in Fagus sylvatica Embryo

Cited by 40 publications

References 15 publications

Changes in endogenous abscisic acid levels during dormancy release and maintenance of mature seeds: studies with the Cape Verde Islands ecotype, the dormant model of Arabidopsis thaliana

Changes in endogenous abscisic acid levels during dormancy release and maintenance of mature seeds: studies with the Cape Verde Islands ecotype, the dormant model of Arabidopsis thaliana

Dormancy termination of western white pine ( Pinus monticola Dougl. Ex D. Don) seeds is associated with changes in abscisic acid metabolism

Storage compounds, ABA and fumarase in Fagus sylvatica embryos during stratification

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