Changed pectinase synthesis by aggregated mycelium of some Aspergillus niger mutants

Leuchtenberger, A.; Mayer, Georg

doi:10.1016/0141-0229(92)90020-o

Cited by 11 publications

(2 citation statements)

References 14 publications

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“…Concerning genetic improvement, Leuchtenberger and Mayer (16) reported two and threefold increases of PG and PL production in mutants of Aspergillus niger. Fernandes-Salomão et al (13) obtained mutants of Penicillium expansum with a two-fold increase in PL and PG production, as compared with the original strain.…”

Section: Introductionmentioning

confidence: 99%

Screening and genetic improvement of pectinolytic fungi for degumming of textile fibers

Molina

Pelissari

Vitorello

2001

Braz. J. Microbiol.

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Aiming at contributing to technological improvements in plant fiber processing methods, this paper reports research work on the obtainment of more efficient pectinase-producing fungi strains. More specifically, this work reports the analysis of 18 strains of filamentous fungi, with the purpose of obtaining enzymes for textile fibers degumming. The strains were evaluated for production of pectinolytic enzymes under several growth conditions (culture medium and growth temperature). Production of pectinases was measured by an enzymatic index (EI) in solid pectin medium. Among the tested strains, Penicillium chrysogenum IFO 4626 (Q 176) showed the best performance. Genetic improvement of this strain was carried out to increase its pectinase production, while keeping cellulase activity down to a negligible level, since cellulases are known to decrease the resistance of the fiber. Variability was induced through several cycles of mutation and selection by exposing conidea to ultraviolet light (UV). We selected 39 out of 390 isolated colonies. Resulting mutants produced nine times more pectin lyase (PL) than the original strain in terms of PL specific activity, and five times more in terms of PL activity (i.e. mmoles liberated per minute of reaction per mL of medium). Periodically, mutant performance was evaluated in solid pectin medium. Genetic stability was maintained for four years after isolation.

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Section: Introductionmentioning

confidence: 99%

Screening and genetic improvement of pectinolytic fungi for degumming of textile fibers

Molina

Pelissari

Vitorello

2001

Braz. J. Microbiol.

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“…Polygalacturonase production has been optimized using submerged culture by Aguilar and Huitron (1987), Leuchtenberger and Mayer (1992) and Friedrich et al (1989). However, industrial production of polygalacturonase is generally carried out by semi-solid fermentation, because this fermentation gives higher activity of the enzyme than submerged fermentation (Pereira et al 1993).…”

Section: Introductionmentioning

confidence: 99%

Production and optimization of polygalacturonase from mango (Mangifera indica L.) peel using Fusarium moniliforme in solid state fermentation

Kumar

Varakumar

Obulam

2010

World J Microbiol Biotechnol

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Mango peel is one of the major wastes from fruit processing industries, which poses considerable disposal problems and ultimately leads to environmental pollution. The objective of the current research was to determine the significant parameters on the production of polygalacturonase from mango peel which is a major industrial waste. Solid state culture conditions for polygalacturonase production by Fusarium moniliforme from dried mango peel powder were optimized by Taguchi's L-18 orthogonal array experimental design methodology. Eight fungal metabolic influencing variables, viz. temperature, mango peel, inoculum, peptone, ammonium nitrate (NH 4 NO 3) , magnesium sulphate (MgSO 4 ), zinc sulphate (ZnSO 4 ) and potassium dihydrogen phosphate (KH 2 PO 4 ) were selected to optimize polygalacturonase production. The optimized parameters composed of temperature (30°C), mango peel (6.5%, g, w/v), inoculum (8%, ml, v/ v), peptone (1%, g, w/v), NH 4 NO 3 (0.60%, g, w/v), MgSO 4 (0.05%, g, w/v), ZnSO 4 (0.06%, g, w/v) and KH 2 PO 4 (0.4%, g, w/v). Based on the influence of interaction of fermentation components of fermentation, these could be classified as the least significant and the most significant at individual and interaction levels. The temperature, inoculum level, mango peel substrate and KH 2 PO 4 showed maximum production impact at optimized conditions. From the optimized conditions the polygalacturonase activity was maximized to 43.2 U g -1 .

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Ausgewählte Methoden und Verfahren der Biotechnologie

Ruttloff

Proll

Leuchtenberger³

1997

Lebensmittel-Biotechnologie Und Ernährung

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Changed pectinase synthesis by aggregated mycelium of some Aspergillus niger mutants

Cited by 11 publications

References 14 publications

Screening and genetic improvement of pectinolytic fungi for degumming of textile fibers

Screening and genetic improvement of pectinolytic fungi for degumming of textile fibers

Production and optimization of polygalacturonase from mango (Mangifera indica L.) peel using Fusarium moniliforme in solid state fermentation

Ausgewählte Methoden und Verfahren der Biotechnologie

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