Change of steroid receptor expression in the posterior vaginal wall after local estrogen therapy

Fuermetz, Anne; Schoenfeld, M. J.; Ennemoser, Stefanie; Muetzel, Elisabeth; Jeschke, Udo; Jundt, Katharina

doi:10.1016/j.ejogrb.2015.02.021

Cited by 19 publications

(12 citation statements)

References 16 publications

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“…Comparing pre-and postmenopausal expression of the ERs in the vaginal wall using IHC has shown lower ERa expression in postmenopausal women compared to premenopausal. The expression increased in women treated with local estrogen [19], less with systemic administration [20]. These results demonstrate the wellknown clinical effect of estradiol or estriol on the vaginal mucosa of the postmenopausal atrofic changes.…”

Section: Discussionsupporting

confidence: 59%

Sex steroid hormone receptor expression in the vaginal wall in cervical cancer survivors after radiotherapy

et al. 2019

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Background: Sex steroid hormones and their receptors are important in female sexual function. The aim of this study was to investigate the expression and distribution of estrogen receptor (ER)a, ERb, Gprotein-coupled ER-1 (GPER), androgen receptor (AR), progesterone receptor (PR)A, PRB and connective tissue growth factor (CTGF) in the vaginal wall among women who had been treated for cervical cancer with radiotherapy. Material and methods: We included cervical cancer survivors treated with radiotherapy and premenopausal control women of the same age scheduled for benign gynecological surgery. We analyzed the expression and distribution of sex steroid hormone receptors and CTGF in biopsies from the vaginal wall, by real-time PCR and immunohistochemistry (IHC). Serum samples were analyzed for hormone levels and radiation dose at biopsy site were calculated and correlated to levels of the sex steroid hormone receptors. Results: In the cervical cancer survivors (n ¼ 34), we found a lower expression of ERa at both mRNA and protein levels, compared to the control women (n ¼ 37). In the survivors with high radiation dose at biopsy site, the immunostaining of ERa and AR was lower in the epithelium and the stroma, compared to survivors with minimal radiation dose. The later group showed expression of ERa comparable to the control women. The cancer survivors were sufficiently substituted with systemic estradiol with no difference in the serum estradiol levels compared to control women. Conclusions: We found that external radiation reduces the ERa and AR protein expression in the vaginal mucosa, indicating that the vaginal changes in irradiated cervical cancer survivors and the lack of response to hormonal treatment could be due to the decreases in sex steroid hormone receptor expression.

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Section: Discussionsupporting

confidence: 59%

Sex steroid hormone receptor expression in the vaginal wall in cervical cancer survivors after radiotherapy

et al. 2019

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“…We did not do so since this had been well documented previously for the given HRT scheme [10,11]. However, we did document the biological end-organ effects (which was one of the objectives of this study), including atrophic epithelial changes, glycogen absence and changes in ER-α expression [31]. Another criticism may be that we performed the experiments only 60 days after ovariectomy, as described previously [10,11].…”

Section: Discussionmentioning

confidence: 95%

First delivery and ovariectomy affect biomechanical and structural properties of the vagina in the ovine model

et al. 2018

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In sheep, life events including delivery and ovariectomy affect the biomechanical properties of the vagina in a region-specific way. Vaginal delivery mainly affects the distal region by decreasing stiffness and contractility. HRT can reverse the increase in stiffness of the mid-vagina observed after surgical induction of menopause. These observations are in line with scanty biomechanical measurements in comparable clinical specimens.

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“…In the present study, E 2 did not significantly increase or decrease LUM mRNA expression in healthy or POP fibroblasts when the CS load was applied. This result may be explained by the fact that estrogen receptor-α and estrogen receptor-β are expressed in the vaginal wall of postmenopausal women [ 56 ] and that local vaginal ET will increase estrogen receptor-α expression, while estrogen receptor-β expression will remain largely unchanged [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of mechanical stretching on the morphology of extracellular polymers and the mRNA expression of collagens and small leucine-rich repeat proteoglycans in vaginal fibroblasts from women with pelvic organ prolapse

et al. 2018

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To determine the effect of mechanical stretching load and the efficacy of postmenopausal estrogen therapy (ET) on pelvic organ prolapse (POP), vaginal fibroblasts isolated from postmenopausal women with or without POP were subjected to 0.1-Hz uniaxial cyclic mechanical stretching (CS) with 10% elongation and 10−8 M 17-β-estradiol (E2) treatment. We investigated the morphological characteristics of extracellular polymers using scanning electron microscopy (SEM) and monitored the mRNA expression of type I collagen (COL I) and type III collagen (COL III) as well as the small leucine-rich proteoglycan (SLRP) family members decorin (DCN), biglycan (BGN), fibromodulin (FMO), and lumican (LUM), using real-time quantitative polymerase chain reaction (RT-PCR). Using SEM, certain viscoelastic polymers were found to be randomly distributed among fibroblasts, which for normal fibroblasts formed clusters of plum flower-like patterns under static-culture conditions and resembled stretched strips when stretched in culture, whereas polymers among POP fibroblasts resembled stretched strips under static-cultured conditions and presented broken networks when stretched in culture. RT-PCR revealed that COL I, DCN, BGN, FMO, and LUM mRNA expression was significantly higher in POP than in normal fibroblasts under static-culture condition. Following CS, COL I and BGN mRNA expression was significantly up-regulated in normal fibroblasts, and DCN and FMO mRNA expression was down-regulated in POP fibroblasts. Following concomitant CS and E2 treatment, significantly elevated COL I and DCN mRNA expression was observed in normal fibroblasts, and significantly elevated COL I and BGN mRNA expression was observed in POP fibroblasts. COL III mRNA expression was not significantly different between the POP and normal group, and CS did not significantly affect expression in either group, though COL III was down-regulated in normal fibroblasts concomitantly treated with E2 and CS. We conclude that the morphological distribution of extracellular polymers in POP fibroblasts exhibited higher sensitivity and lower tolerance to stretching loads than do normal fibroblasts. These mechanical properties were further reflected in the transcription of COL I. Defects in the compensatory function of BGN for DCN and LUM for FMO exist in POP fibroblasts, which further affect the structure and function of COL I in response to stretching load, ultimately resulting in abnormal reconstruction of pelvic supportive connective tissues and the occurrence of POP. ET can maintain stretching-induced elevations in COL I and DCN transcription in healthy women and improve stretching-induced COL I, DCN, BGN, and FMO transcriptional changes in POP women to prevent and improve POP. Only down-regulated COL III transcription was observed upon concomitant CS and E2 treatment in normal fibroblasts, which suggests that the tensile strength, not the elasticity, of the supportive connective tissues is damaged in POP and that the higher tensile strength induced by ET in healthy fibroblasts ...

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Change of steroid receptor expression in the posterior vaginal wall after local estrogen therapy

Cited by 19 publications

References 16 publications

Sex steroid hormone receptor expression in the vaginal wall in cervical cancer survivors after radiotherapy

Sex steroid hormone receptor expression in the vaginal wall in cervical cancer survivors after radiotherapy

First delivery and ovariectomy affect biomechanical and structural properties of the vagina in the ovine model

Effects of mechanical stretching on the morphology of extracellular polymers and the mRNA expression of collagens and small leucine-rich repeat proteoglycans in vaginal fibroblasts from women with pelvic organ prolapse

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