Change of Recipient Corneal Endothelial Cells After Non-Descemet's Stripping Automated Endothelial Keratoplasty in a Rabbit Model

Abstract

After non-Descemet's stripping automated endothelial keratoplasty, the CEEB showed decreased density, loss of pump function, apoptosis and changed morphology. However, the interface opacity was not significantly greater compared with DSAEK eyes.

Graft rejection episodes after keratoplasty in Japanese eyes

“…Several reports support the advantages of nDSAEK, particularly for failed PK, as stripping Descemet's membrane of failed PK may cause PK wound separation or weakness [27][28][29] . The histology and in vivo observation of the residual host endothelial cells after nDSAEK have already been thoroughly studied, including decreased density, loss of pump function, apoptosis, and altered morphology in a rabbit model by in vivo confocal microscopy 34 . Additionally, in vivo laser confocal microscopy in human studies after nDSAEK can detect subclinical corneal abnormalities, including subepithelial haze, host-recipient interface haze, host stromal needle-shaped materials, www.nature.com/scientificreports/ and host-recipient interface particles with characteristic giant particles 35 .…”

Graft rejection episodes after keratoplasty in Asian eyes

“…[23][24][25] The histology and in vivo observation of the residual host endothelial cells after nDSAEK have already been thoroughly studied, including decreased density, loss of pump function, apoptosis, and altered morphology in a rabbit model by in vivo confocal microscopy. [30] Additionally, in vivo laser confocal microscopy in human studies after nDASEK can detect subclinical corneal abnormalities, including subepithelial haze, host-recipient interface haze, host stromal needle-shaped materials, and host-recipient interface particles with characteristic giant particles. [31] Using immunohistochemistry and transmission electron microscopy, 2 weeks after nDSAEK in a rabbit model, host endothelial cells appeared to be morphologically altered, occasionally detached from the adjacent Descemet membrane, extending into the graft stroma or engul ng strands of the grafted stroma at the interface.…”