Change of Cu,Zn-superoxide Dismutase Activity of Guinea Pig Lung in Experimental Asthma

Nishida, Shozo; Teramoto, Kazuhiro; Kimoto-Kinoshita, Saori; Tohda, Yuji; Nakajima, Shigenori; Tomura, Takanori; Irimajiri, Kiyohiro

doi:10.1080/10715760210872

Cited by 29 publications

(16 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is well known that MDA production is a suitable index of membrane lipid peroxidation and cell damage [7,8] and that consumption of endogenous antioxidant enzymes, like SOD, is a typical effect of oxidative stress [5]. In our experiments, antigen challenge signifi cantly increased MDA production (Fig.…”

Section: Resultssupporting

confidence: 48%

Activation of cannabinoid receptors reduces allergen-induced oxidative stress damage during asthma-like reaction in sensitised guinea-pigs

et al. 2007

View full text Add to dashboard Cite

Section: Resultssupporting

confidence: 48%

Activation of cannabinoid receptors reduces allergen-induced oxidative stress damage during asthma-like reaction in sensitised guinea-pigs

et al. 2007

View full text Add to dashboard Cite

“…Level of ROS has been shown to be increased in the airways of OVA-challenged animals (Nishida et al, 2002;Zhang et al, 2002;Lee et al, 2004dLee et al, , 2005Lee et al, , 2006a. ROS released by inflammatory cells infiltrating the airways play an important role in the airway tissue injury observed in asthma (Dworski, 2000).…”

Section: Discussionmentioning

confidence: 99%

A novel thiol compound, N-acetylcysteine amide, attenuates allergic airway disease by regulating activation of NF-κB and hypoxia-inducible factor-1α

Lee

Kim²,

Park³

et al. 2007

Exp Mol Med

View full text Add to dashboard Cite

Reactive oxygen species (ROS) play an important role in the pathogenesis of airway inflammation and hyperresponsiveness. Recent studies have demonstrated that antioxidants are able to reduce airway inflammation and hyperreactivity in animal models of allergic airway disease. A newly developed antioxidant, small molecular weight thiol compound, N-acetylcysteine amide (AD4) has been shown to increase cellular levels of glutathione and to attenuate oxidative stress related disorders such as Alzheimer's disease, Parkinson's disease, and multiple sclerosis. However, the effects of AD4 on allergic airway disease such as asthma are unknown. We used ovalbumin (OVA)-inhaled mice to evaluate the role of AD4 in allergic airway disease. In this study with OVA-inhaled mice, the increased ROS generation, the increased levels of Th2 cytokines and VEGF, the increased vascular permeability, the increased mucus production, and the increased airway resistance in the lungs were significantly reduced by the administration of AD4. We also found that the administration of AD4 decreased the increases of the NF-κB and hypoxia-inducible factor-1α (HIF-1α ) levels in nuclear protein extracts of lung tissues after OVA inhalation. These results suggest that AD4 attenuates airway inflammation and hyperresponsiveness by regulating activation of NF-κB and HIF-1α as well as reducing ROS generation in allergic airway disease.

show abstract

“…The sonicated samples were subsequently centrifuged at 1100g for 10 min. SOD activity was measured in the supernatants as previously described (Beauchamp and Fridovich, 1971;Nishida et al, 2002), with some modifications. Briefly, a competitive inhibition assay was performed that used xanthine-xanthine oxidase-generated superoxide to reduce nitro blue tetrazolium (NBT) to blue tetrazolium salt.…”

Section: Methodsmentioning

confidence: 99%

A Newly Identified Role for Superoxide in Inflammatory Pain

Wang¹,

Porreca

Cuzzocrea

et al. 2004

J Pharmacol Exp Ther

350

361

View full text Add to dashboard Cite

Novel classes of pain-relieving molecules are needed to fill the void between nonsteroidal anti-inflammatory agents and narcotics. Our studies have identified superoxide as a novel mediator of hyperalgesia (clinically defined as an augmented sensitivity to painful stimuli) and have exposed potential pathways through which this radical modulates the hyperalgesic response. The role of superoxide in pain was elucidated using a superoxide dismutase mimetic, M40403 [a manganese(II) complex with a bis(cyclo-hexylpyridine-substituted) macrocyclic ligand]. Intraplantar injection of carrageenan in rats led to timedependent development of peripheral inflammation [measured parameters of inflammation included paw edema, cytokine release in the paw exudates, nitrotyrosine formation (a marker of peroxynitrite formation and oxidative stress), and poly-ADPribose-polymerase activation (the nuclear enzyme activated by superoxide/peroxynitrite)] and hyperalgesia. M40403 blocked all measured parameters of inflammation and hyperalgesia. Furthermore, when given therapeutically (2 h after the induction of hyperalgesia) either by intravenous or intrathecal administration, M40403 but not its inactive congener M40404 inhibited hyperalgesia with a rapid onset of action. Our results also show that, at the level of the spinal cord and time of peak hyperalgesia, endogenous manganese superoxide dismutase was nitrated and subsequently deactivated, losing its capacity to remove superoxide. The antihyperalgesic effects of M40403 were not reversed by naloxone excluding the potential involvement of an opiate pathway. Collectively, these studies have unraveled a critical role for superoxide in the nociceptive signaling cascade both peripherally and centrally. The discovery of this pathway opens a new therapeutic strategy for the development of novel nonnarcotic antihyperalgesic agents.

show abstract

Change of Cu,Zn-superoxide Dismutase Activity of Guinea Pig Lung in Experimental Asthma

Cited by 29 publications

References 20 publications

Activation of cannabinoid receptors reduces allergen-induced oxidative stress damage during asthma-like reaction in sensitised guinea-pigs

Activation of cannabinoid receptors reduces allergen-induced oxidative stress damage during asthma-like reaction in sensitised guinea-pigs

A novel thiol compound, N-acetylcysteine amide, attenuates allergic airway disease by regulating activation of NF-κB and hypoxia-inducible factor-1α

A Newly Identified Role for Superoxide in Inflammatory Pain

Contact Info

Product

Resources

About