Change involving a viral membrane glycoprotein during morphogenesis of group B arboviruses

Fusion activity of flaviviruses: comparison of mature and immature (prM-containing) tick-borne encephalitis virions

“…The assembly of ftaviviruses involves the intracellular generation of 'immature' virions which contain prM rather than M (Shapiro et al, 1972;Rice et al, 1986). The proteolytic cleavage of prM as a last step in virus maturation seems to be necessary to yield fully infectious virus (Wengler & Wengler, 1989).…”

“…Immature virions found in infected cells contain an additional glycosylated protein (prM) which is the precursor of the M protein (reviewed by Rice et al, 1986). The proteolytic processing of prM to yield M apparently represents a late event in the maturation of flaviviruses and is required to obtain fully infectious virions (Shapiro et al, 1972;Wengler & Wengler, 1989). Cross-linking experiments indicate that the cleavage of prM may be associated with a rearrangement of the oligomeric structures on the surface of the virus (Wengler 0001-0016 © 1991 SGM F. Guirakhoo and others & Wengler, 1989).…”

Envelope Protein of the Flavivirus Kunjin is Apparently Not Glycosylated

“…The glycoprotein GP 19 has been labelled with radioactive carbohydrate or amino acids and detected in slowly sedimenting haemagglutinating particles and in some preparations of virions (Stollar, 1969;Shapiro et al, 1971;Trent & Qureshi, 1971;Shapiro et al, 1972;Westaway, 1973Westaway, , 1975Boulton & Westaway, 1976). Since the analyses presented in this (Wright et al, 1980).…”

“…The E proteins of several flaviviruses, e.g. dengue type 2 (Stollar, 1969;Shapiro et al, 1972), Japanese encephalitis (JE) (Shapiro et al, 1973), Saint Louis encephalitis (SLE) (Qureshi & Trent, 1973) and Kunjin (Boulton & Westaway, 1976;Westaway & Shew, 1977) have been reported to be glycosylated, usually on the basis of their incorporation of radioactive carbohydrates into the envelope protein. However, the possibility that the radioactive label in the carbohydrate may have been incorporated into proteins and 0022-1317/82/0000-4838 $02.00 © 1982 SGM Purification of radioactively labelled viruses.…”

“…Envelope protein (E) and a membrane-like protein (M) are found in both virions and slowly sedimenting haemagglutinating particles, core protein (C) is found only in virions, and a fourth polypeptide, a glycoprotein (GP 19), is detected both in slowly sedimenting haemagglutinating particles and under some conditions in virions (Stollar, 1969;Shapiro et al, 1971;Trent & Qureshi, 1971;Shapiro et al, 1972;Westaway, 1973Westaway, , 1975Boulton & Westaway, 1976). We are currently investigating the synthesis of Kunjin virus-specified glycoproteins in infected cells, and comparing the properties of E and GPI9 from virions and slowly sedimenting haemagglutinating particles with those of their likely precursors in infected cells (Wright et al, 1980(Wright et al, , 1981.…”