Change in the Bacterial Community of Natural River Biofilm during Biodegradation of Aniline-Derived Compounds Determined by Denaturing Gradient Gel Electrophoresis

Araya, Rubén; Yamaguchi, Naohito; Tani, Katsuji; Nasu, Masao

doi:10.1248/jhs.49.379

Cited by 15 publications

(16 citation statements)

References 28 publications

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“…According to the investigation by Araya et al [22] the bacterial community structure of biofilm formed on unglazed ceramic slides submerged in natural river water was similar to those formed on natural stones in river beds. This study thus submerged the unglazed ceramic disks to simulate natural stones for biofilm colonization in order to obtain fixed surface area for diazinon degradation tests.…”

Section: Growth Of River Biofilmsmentioning

confidence: 92%

“…Wolfaardt et al [20] investigated the degradation of the herbicide diclofop methyl by an algal-bacterial consortium and found the microalga had a beneficial effect by producing extracellular matter permitting the co-metabolism of the herbicide by the bacteria. River biofilm communities were found to be able to sorb and metabolize aniline and two herbicides, atrazine and diclofop methyl [21,22]. Schorer and Eisele [23] found river biofilms were able to sorb large amounts of heavy metals and hydrophobic organic pollutants.…”

Section: Introductionmentioning

confidence: 99%

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The Role of Naturally Occurring River Biofilms on the Degradation Kinetics of Diazinon

Tien¹,

Chuang²,

Chen³

2011

CLEAN Soil Air Water

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River biofilms are dominant riverine biota with diverse microorganisms. They have been found to contribute greatly to river self-purification for removal of nutrients and organic matter. This study intended to investigate the ability of naturally occurring river biofilms with changing seasons for the removal of the organophosphorus pesticide diazinon. Natural river biofilms from spring showed higher ability to remove diazinon (99.9% removal) than those from winter (77%) with light exposure. In contrast to control sets without biofilms under irradiation, 27% of diazinon removal in spring and 22% in winter may result from microbial activity within biofilms. Removal of diazinon by river biofilms could be attributed mostly to degradation due to low sorption capacity of biofilms. Spring biofilms had higher dissipation rates (0.265 and 0.486 d À1 for biofilms with different growth periods) than winter ones (0.099 and 0.119 d À1 ) according to first order model. Higher ability of diazinon removal by spring biofilms may be explained by their higher bacterial and algal biomass comparing to winter biofilms. Naturally occurring river biofilms played a significant role in degradation of diazinon, particularly for those grown in spring. Their potential for use in the treatment of diazinon-contaminated water has been demonstrated.

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Section: Growth Of River Biofilmsmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

The Role of Naturally Occurring River Biofilms on the Degradation Kinetics of Diazinon

Tien¹,

Chuang²,

Chen³

2011

CLEAN Soil Air Water

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“…Sorption of large amounts of nickel by natural river biofilms was observed by Hitchcock et al (2009). Sorption and metabolism of aniline, atrazine, diclofop methyl, diazinon, carbofuran, carbaryl and methomyl by river biofilm communities has also been found (Lawrence et al 2001;Araya et al 2003;Tien et al 2011Tien et al , 2013. Thus, river biofilms might contain microorganisms with the ability to degrade organic contaminants such as pesticides.…”

Section: Introductionmentioning

confidence: 92%

“…The reasons to use unglazed ceramic discs for colonization of river biofilms were not only to obtain a fixed surface area for the tests, but also to have the bacterial community structure similar to those formed on natural stones in river beds (Araya et al 2003). Fifty ceramic discs were hung on a paint-covered steel rack with plastic strings and submerged in river water to a 1 m depth, parallel to the current flow, for 21 days under the Se-Kung Bridge (23°6 0 26 00 N, 120°12 0 18 00 E) of the Tsen-Wen River, which has intensive agriculture activity nearby.…”

Section: Acclimatizing Natural River Biofilmsmentioning

confidence: 99%

The ability of immobilized bacterial consortia and strains from river biofilms to degrade the carbamate pesticide methomyl

Chen

Wang

et al. 2014

Int. J. Environ. Sci. Technol.

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Complex microbial communities from river biofilms might contain microorganisms capable of degrading xenobiotic pollutants such as pesticides (e.g. methomyl, which is commonly detected in rivers). Therefore, this study was used to determine the methomyl degradation potential of bacteria consortia and single bacterial strains acclimatized and isolated from natural river biofilms to provide biomaterials for bioremediation of water that is contaminated with methomyl. Natural river biofilms were culture enriched with methomyl as the sole carbon source to obtain acclimatized bacterial consortia and single bacterial strains. The microbial consortium on the ceramic discs was able to remove 91 % of added methomyl (50 mg l -1 ) in 7 days. The longer-acclimatized bacterial consortium on loofah sponges removed methomyl more quickly than the shorter-acclimatized consortium, but both had similar removal capabilities (i.e. 92.4 and 92.2 %). This finding suggested that the former might contain more methomyl degraders than the latter. However, after preservation at 25, 4 and -20°C for 1 or 3 months, the methomyl degradation ability of the bacterial consortia decreased significantly, indicating loss of methomyl degraders during preservation. Three bacterial species were isolated from acclimatized river biofilms, and only one species, identified as Sphingomonas sp., was able to remove methomyl, with a 7-day removal rate of 44.7 % when sugar was added and of 32.5 % when no sugar was added. These results suggested that an additional carbon source might slightly improve the ability of Sphingomonas sp. to degrade methomyl. Acclimatized bacterial consortia have a higher potential for treating methomyl-contaminated water than isolated bacterial species.

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“…Rate of growth of biofilm depends on the underlying substrate (Taylor et al 1997). Tests in the Kanzaki River in Osaka, (Araya et al 2003) showed that glass was the best substrate, stone and ceramic were second equal, and polycarbonate was third. Silica tubes are the usual sample containers in ESR measurements, and could be an approximation of glass.…”

Section: Methodsmentioning

confidence: 99%

Preliminary results of using ESR to examine biofilms

Whitehead¹,

Atushi²,

Tazaki³

et al. 2004

Electro Journal of Biotech

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This preliminary work shows ESR (Electron SpinResonance) can be used to detect biofilms, particularly from Fe-metabolising bacteria. A film was detected by ESR as early as 1 day, hence possibly more sensitively than by fluorescent methods. Films can probably be detected as early as one hour. Spectra contain a very broad peak at g=2.13, probably due to ferrihydrite. Results of field experiments from streams and ponds in New Zealand and Japan, particularly the Minoh River, showed a general increase of ferrihydrite with time. Loss by exfoliation was later than 20 days. The rate of accumulation was faster in a nutrient-rich stagnant pond. Hematite (g=4.3) was often observed, magnetite (g=9) once, and usually small amounts of a common bacterial decay product. The latter was detected for at least 18 months film storage. ESR is a particularly good tool for observing the growth of oxic biofilms containing *Corresponding author Fe-metabolising bacteria, and should be just as sensitive for observing Mn-metabolising bacteria in reducing conditions.

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Change in the Bacterial Community of Natural River Biofilm during Biodegradation of Aniline-Derived Compounds Determined by Denaturing Gradient Gel Electrophoresis

Cited by 15 publications

References 28 publications

The Role of Naturally Occurring River Biofilms on the Degradation Kinetics of Diazinon

The Role of Naturally Occurring River Biofilms on the Degradation Kinetics of Diazinon

The ability of immobilized bacterial consortia and strains from river biofilms to degrade the carbamate pesticide methomyl

Preliminary results of using ESR to examine biofilms

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