Change in Microbiological Conditions of Lawn Grass Root Zones as a Result of Fertilization with Sewage Sludge

Kurowski, T.P.; Przemieniecki, Sebastian Wojciech; Grabowski, Kazimierz; Damszel, Marta; Kwiatkowska, Edyta

doi:10.15244/pjoes/62643

Cited by 5 publications

(4 citation statements)

References 19 publications

(23 reference statements)

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“…The taxonomic diversities of the analysed OTUs were determined with the use of Simpson dominance (λ), Shannon diversity index (H’), and Pielou’s evenness index (J′) 67 – 69 . Domination classes were determined according to previous work Przemieniecki et al 70 for bacteria and Kurowski et al 71 for fungi.…”

Section: Methodsmentioning

confidence: 99%

“…The taxonomic diversities of the analysed OTUs were determined with the use of Simpson dominance (λ), Shannon diversity index (H'), and Pielou's evenness index (J′) [67][68][69] . Domination classes were determined according to previous work Przemieniecki et al 70 for bacteria and Kurowski et al 71 for fungi. The normality of the distribution of the obtained results was tested (Shapiro-Wilk test) and equality of variances was tested Levene's Test.…”

Section: Statistical Calculationsmentioning

confidence: 99%

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Interspecies and temporal dynamics of bacterial and fungal microbiomes of pistil stigmas in flowers in holoparasitic plants of the Orobanche series Alsaticae (Orobanchaceae)

Ruraż

Przemieniecki

Piwowarczyk

2023

Sci Rep

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Little is known about the microbiomes of flower parts, and even less information is available regarding these microorganisms’ colonization of specific niches in parasitic plants. We investigate the temporal interspecies dynamics of the parasitic plants microbiome of flower stigmas in two stages of development: immature stigmas in flower buds and mature stigmas in opened flowers. We compared two related holoparasitic Orobanche species from localities approximately 90 km apart and characterize their bacterial and fungal communities using 16S rRNA gene and ITS sequences, respectively. We identified from 127 to over 228 OTUs per sample for fungi, sequences belonging to genera: Aureobasidium, Cladosporium, Malassezia, Mycosphaerella, and Pleosporales, constituting approximately 53% of the community in total. In the bacterial profile, we recorded 40 to over 68 OTUs per sample consisting of Enterobacteriaceae, and genera Cellulosimicrobium, Pantoea, and Pseudomonas spp., with an approximately 75% frequency. In microbial communities, higher numbers of OTUs colonizing mature stigmas were recorded than in immature. This implies that the dynamics and concurrence of microbial communities were different between O. alsatica and O. bartlingii and underwent significant changes during flower development. To the best of our knowledge, is the first study of the interspecies and temporal dynamics of the bacterial and fungal microbiomes of pistil stigmas in flowers.

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Section: Methodsmentioning

confidence: 99%

Section: Statistical Calculationsmentioning

confidence: 99%

Interspecies and temporal dynamics of bacterial and fungal microbiomes of pistil stigmas in flowers in holoparasitic plants of the Orobanche series Alsaticae (Orobanchaceae)

Ruraż

Przemieniecki

Piwowarczyk

2023

Sci Rep

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“…In ensiled grasses, the most negative effects are exerted by Penicillium species, which produce patulin, ochratoxin A, and roquefortine . Epiphytic and endophitic fungi of the genera Fusarium and Penicillium constitute the predominant microbiota during the growing season therefore grass used as green fodder and a substrate converted to silage is highly susceptible to contamination with toxin‐producing fungi and mycotoxins …”

Section: Introductionmentioning

confidence: 99%

“…Obligate or facultative heterofermentative bacteria can therefore be used to ensile herbage with high moisture content and relatively rich microbiota. Heterofermentative bacteria ferment pentoses and hexoses, which prevents silage deterioration caused by undesirable microorganisms …”

Section: Introductionmentioning

confidence: 99%

The effect of an additive containing three Lactobacillus species on the fermentation pattern and microbiological status of silage

et al. 2019

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BACKGROUND: Appropriate combinations of lactic acid bacteria (LAB) strains should be selected to optimize the ensiling process, and the additives should be adjusted to the ensiled forage crops. The aim of this study was to determine the effect of inoculation with three Lactobacillus species on the chemical parameters and microbiological quality (beneficial and harmful microbiota) of grass silage. RESULTS: Three species: L. paracasei (LPa), L. brevis (LB) and L. plantarum (LPl), isolated from sugar beet silage and characterized based on 16S rDNA sequences and biochemical parameters, were analyzed in the study. Single strains and their combinations were used as silage inoculants. The basic chemical and microbiological (qPCR) parameters of silages were determined. Based on the results of agglomerative hierarchical clustering (AHC) and principal component analysis (PCA) it was determined that silages inoculated with single LAB strains and LPa + LB and commercial additive (0+) were the best quality ones, particularly with regard to microbiological parameters and they effectively lowered the pH value. A consortium of three Lactobacillus species had no influence on silage quality, whereas LPa + LB and LPl + LB combinations as well as a commercial additive exerted positive effects. Inoculation inhibited the growth of toxin-producing fungi.CONCLUSION: Only the appropriate LAB composition can improve the quality of the ensiled material (antagonistic relationship). Only the LPa + LB combination was able to improve the value of low dry silage; nevertheless, almost all combinations were able to reduced concentrations of toxin-producing fungi. DISCUSSIONIn this experiment, grass herbage was obtained from the fourth cut due to higher expected fungal loads (in particular Fusarium spp.). A short regrowth period resulted in high crude protein content.

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Long term growth of crop plants on experimental plots created among slag heaps

Halecki

Klatka

2018

Ecotoxicology and Environmental Safety

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Change in Microbiological Conditions of Lawn Grass Root Zones as a Result of Fertilization with Sewage Sludge

Cited by 5 publications

References 19 publications

Interspecies and temporal dynamics of bacterial and fungal microbiomes of pistil stigmas in flowers in holoparasitic plants of the Orobanche series Alsaticae (Orobanchaceae)

Interspecies and temporal dynamics of bacterial and fungal microbiomes of pistil stigmas in flowers in holoparasitic plants of the Orobanche series Alsaticae (Orobanchaceae)

The effect of an additive containing three Lactobacillus species on the fermentation pattern and microbiological status of silage

Long term growth of crop plants on experimental plots created among slag heaps

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