Change in hepatic antioxidant defense system with liver injury development in rats with a single α-naphthylisothiocyanate intoxication

Resveratrol effectively attenuates α-naphthyl-isothiocyanate-induced acute cholestasis and liver injury through choleretic and anti-inflammatory mechanisms

“…In the present study, an enhancement in the hepatic activity of MPO, an index of tissue neutrophil infiltration, occurred after ANITinduced liver injury. These data confirm previously reported results [30,31] . Resveratrol administered intraperitoneally to ANIT-treated rats after the appearance of liver injury significantly reduced the increase in hepatic MPO activity.…”

Protective effect of Danning tablet on acute livery injury with cholestasis induced by α-naphthylisothiocyanate in rats

“…It has been suggested that hepatic antioxidant defense system disruption might be associated with ANIT-induced liver injury in rats Ohta et al, 1999;Kodali et al, 2006). Hepatic antioxidant defense system disruption is characterized by increased LPO and/or altered non-enzymatic and enzymatic antioxidants including CAT, SOD, GPx, GST and GSH.…”

Evaluation of the protective effect of Zhi-Zi-da-Huang decoction on acute liver injury with cholestasis induced by α-naphthylisothiocyanate in rats

“…Finally, the released free ANIT selectively damages bile duct epithelium and causes intrahepatic cholestasis (Dietrich et al, 2001). According to the current understandings, biochemical and pathological changes induced by ANIT toxicity include elevated levels of serum bile acids and bilirubin, increased activities of ALT, AST and other serum enzymes and the appearance of histopathological lesions (Cui et al, 2009;Ohta et al, 1999). In the present study, rats exhibited obvious liver injury with cholestasis at 48 h after treated once with ANIT (75 mg/kg B.W.).…”

“…At 12 h after the fifth administration of test drugs, all rats except the normal control group were intragastrically treated with ANIT dissolved in peanut oil at a dose of 75 mg/kg B.W., and the normal control group received peanut oil in an equal volume. The dose and time of intoxication based on the related references (Ohta et al, 2006;Kongo et al, 1999). Forty-eight hours after ANIT treatment, blood samples were collected from the fosse orbital vein for assays of serum enzymes and components.…”

“…The ANIT-induced liver injury in rats has been reported to be associated with lipid peroxidation (Kongo et al, 1999;Dietrich et al, 2001). The lipid peroxidation might occur via oxygen radicals derived from neutrophils infiltrated into the liver tissue of ANIT-intoxicated rats (Kongo et al, 1999).…”