Change in Free Radical and Antioxidant Enzyme Levels in the Patients Undergoing Open Heart Surgery with Cardiopulmonary Bypass

Abstract

Objective. The purpose of this study is to determine the changes in oxidative damage and antioxidant parameters in open heart surgeries with cardiopulmonary bypass (CPB) in preoperative and early postoperative periods. Methods. A total of three consecutive arterial blood samples were obtained from the patients in the study group, in preoperative, early postoperative, and postoperative periods, respectively. Oxidative damage indicator (MDA) and antioxidant indicators (GPx, GSH, CAT, and SOD) were examined. Results. A statistically significant increase was observed in MDA level in postoperative period compared to preoperative and early postoperative periods. GSH levels and CAT activities increased significantly in early postoperative and postoperative periods. Analyses revealed an increase in GPx and SOD enzyme activities only in the postoperative period. Conclusion. Even though the increase in MDA level was suppressed by the increased GSH level and CAT activity like in early postoperative period, efficiency can be brought for the increases in insufficient significant antioxidant parameters in postoperative period by administering antioxidant supplements to the patients and thus the increase in MDA in postoperative period can be significantly suppressed.

Glutathione Blood Concentrations: A Biomarker of Oxidative Damage Protection during Cardiopulmonary Bypass in Children

“…Furthermore, GSH levels measured at the end of the CPB procedure positively correlated with CPB, cross-clamp duration, and PaO 2 , whereas a negative correlation was observed with body core temperature. These results are, in part, in agreement with previous observations in adults and in children [15,27], showing increased GSH levels in the postoperative period and in the different CPB phases. Discrepancies are in relation to the different monitoring time points, CPB procedure, and patient recruitment [15].…”

SIRT1 exhibits antioxidative effects in HT22 cells induced by tert‐butyl alcohol

“…These findings indicate that TBA has oxidative activities through direct ROS generating and suppressing the activities of endogenous antioxidant enzymes. Similar changes in free radical and endogenous antioxidant enzyme levels were found in alcoholic liver disease, reperfusion injury of rat retina and the patients undergoing open heart surgery with cardiopulmonary bypass …”

“…The balance between oxidants and antioxidants is important to the physiological activity and function of the cell. Excessive ROS, triggered by exogenous substance itself or its metabolites, may destroy virtually any molecule they encounter, and then endogenous antioxidant genes, such as SOD and GSH peroxidase, were activated to scavenge intracellular redundant ROS . SOD, the first defense against free radicals, can distract surplus electrons from free radicals, resulting in the formation of hydrogen peroxide (H 2 O 2 ), which will be decomposed to water and oxygen by GSH peroxidase with itself oxidized to GSSG .…”

Whole Blood Redox Potential Correlates With Progressive Accumulation of Oxygen Debt and Acts as A Marker of Resuscitation in A Swine Hemorrhagic Shock Model

“…Animals were then hemorrhaged by removing 45–60% of their blood volume while maintaining a mean arterial pressure (MAP) of 30–35 mmHg, ultimately targeting an oxygen debt of 80mL/kg. Eight (8) central venous blood samples from the proximal port of the pulmonary artery catheter were taken at regular intervals of oxygen debt and separated into whole blood and plasma aliquots, with measurements of central venous oxygen saturation (ScvO 2 ) recorded as well (ABL800, Radiometer America). These intervals of oxygen debt were: baseline (0), 20, 40, 60, and 80 mL/kg.…”

“…As such, little is known regarding the changes in redox potential during dynamic states of critical illness and injury. While various redox pairs such as lactate-pyruvate or oxidative stress indicators such a thiobarbituric acid reactive substances have been studied in an attempt to measure oxidative stress as an indicator of redox potential (7, 8), such individual markers cannot reflect the system as a whole since there are a large numbers of redox pairs contributing to overall ambient redox potential similar to the many acid-base pairs contributing to pH. In addition, these markers also have their limitations with regard to fully assessing systemic redox state, as they can be affected by the origin of the species measured (e.g.…”