Change in CD34+ cell concentration during peripheral blood progenitor cell collection: effects on collection efficiency and efficacy

Ford, Clyde D.; Greenwood, Jay H.; Strupp, Annie; Lehman, Chris

doi:10.1046/j.1537-2995.2002.00131.x

Cited by 32 publications

(39 citation statements)

References 34 publications

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“…15 However, we have also suggested previously that the end point of harvest yield does add additional confounding variables not related to mobilization such as cell separator collection efficiency. 16 We also attempted to design our study in a manner that would minimize the effect of mobilization regimen variables as confounding factors. As randomized trials have demonstrated the superiority of chemotherapy þ growth factors over growth factors alone, 17 we included only patients receiving both chemotherapy and G-CSF.…”

Section: Discussionmentioning

confidence: 99%

Effect of prior chemotherapy on hematopoietic stem cell mobilization

Ford

Green

Warenski

et al. 2004

Bone Marrow Transplant

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Summary:A number of studies have suggested that prior chemotherapy correlates negatively with the efficiency of hematopoietic stem cell mobilization. However, little data exist with regard to the relative effects of the specific chemotherapeutic drug classes. We retrospectively reviewed the records of 201 consecutive patients with nonmyeloid malignancies undergoing CD34 þ cell mobilization with chemotherapy þ granulocyte colony-stimulating factor (G-CSF). The number of prior chemotherapy courses correlated negatively with the peripheral CD34 þ cell concentration (pCD34) on the first day of collection (Po0.001). No significant correlation was found for age, gender, tumor primary, mobilization chemotherapy regimen, disease status, marrow involvement, prior radiation therapy, or dose and timing of G-CSF administration. When the number of courses of individual classes of chemotherapeutic agents was correlated with pCD34, only exposures to platinum compounds (P ¼ 0.001) and alkylating agents (P ¼ 0.01) were found to be independent negative predictive factors for pCD34. Within classes, DNA crosslinking agents and etoposide appeared possibly more damaging than DNA methylating agents and doxorubicin, respectively. None of the drug classes showed evidence of recovery. We conclude that exposure to chemotherapy, especially platinum compounds and alkylating agents, should be minimized prior to mobilization.

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Section: Discussionmentioning

confidence: 99%

Effect of prior chemotherapy on hematopoietic stem cell mobilization

Ford

Green

Warenski

et al. 2004

Bone Marrow Transplant

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“…Lemmens‐Bernstein‐Brodsky formula was used for patients >50 kg and BMI > =85th percentile . Collection efficiency (CE2) of CD34+ cells was calculated assuming that level of CD34+ in the circulation during the procedure time is stable . The CE2 was calculated using the formula CE2 (%) = product CD34+ cells/kg × BW/ [precollection CD34 + × (TBV ‐ ACD)].…”

Section: Methodsmentioning

confidence: 99%

Comparison between intermittent and continuous leukapheresis protocols for autologous hematopoietic stem cell collections in children

Ali

Chiang

Even‐Or

et al. 2019

J of Clinical Apheresis

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Background Peripheral hematopoietic stem cell (HSC) collections are needed for autologous hematopoietic stem cell transplantation (HSCT). Since 2015, our institution has utilized a secondary chamber mononuclear cell (MNC) protocol on the Spectra Optia apheresis system. Recently, a new continuous mononuclear collection protocol (CMNC) was developed for the same device. As there is limited data available regarding the use of the CMNC protocol in children, we compared collection efficiency (CE2), side effects, and clinical feasibility between the two protocols in patients <18 years old. Study Design and Methods We prospectively collected clinical, laboratory, and technical collection data from HSC collection procedures performed with the Spectra Optia apheresis system utilizing the CMNC protocol. Data were compared to retrospectively collected data utilizing the MNC protocol. Data collection included donor demographics, precollection peripheral CD34+ cell counts, total CD34+ cells collected, collection efficiency, side effects, and collection product characteristics. Results A total of 96 HSC collection procedures were performed on 79 pediatric patients utilizing either the MNC (61 patients) or CMNC (18 patients) protocol. The collection efficiencies were comparable between MNC and CMNC cohorts (52.9% vs 54.9%, P = 0.711). Platelet loss was significantly lower in the CMNC cohort (P = 0.002), especially in children weighing <15 kg. Product volumes were higher with CMNC. No significant collection‐related side effects were noted with either protocol. Conclusions MNC and CMNC protocols have comparable collection efficiencies and are both feasible and safe for the use in children. Centers may choose between the methods depending on clinical needs.

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“…20,22,25 For the present analysis, we embraced the concept of relative efficiency (RE), the ratio between the absolute yield of CD34+ cells in the leukapheresis product (yCD34+) and the initial circulating pool of CD34+ cells (ipCD34+):…”

Section: Collection Efficiencymentioning

confidence: 99%

“…[18][19][20][21] The higher yCD34+ with LVL may result from better extraction of the initial circulating pool of CD34+ but also from intraapheresis recruitment of CD34+ from the marrow compartment. 20,22 Even though LVL is a safe approach, 19 little is known about potential loss of collection efficiency when higher blood volumes are processed.…”

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confidence: 99%