Chang’an II Decoction (肠安 II 号方)-Containing Serum Ameliorates Tumor Necrosis Factor-α-Induced Intestinal Epithelial Barrier Dysfunction via MLCK-MLC Signaling Pathway in Rats

Chen, Ting; Yin, Xiaolan; Kang, Nan; Wang, Xiaoge; Li, Bao-Shuang; Ji, Haijie; Zhang, Yinqiang; Bian, Li-Qun; Zhang, Bei-hua; Wang, Fengyun; Tang, Xudong

doi:10.1007/s11655-019-3034-6

Cited by 5 publications

(2 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…NF-κB mediates the upregulation of MLCK, and subsequently, MLCK promotes the phosphorylation of MLC. Then, pMLC disrupts barrier integrity through disassembling the TJ proteins ( Gu et al, 2016 ; Chen et al, 2019 ). It was noted that W. cibaria MW01 reduced the LPS-dependent translocation of NF-κB into the nucleus, indicating the downregulation of the NF-κB signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

Weissella cibaria Attenuated LPS-Induced Dysfunction of Intestinal Epithelial Barrier in a Caco-2 Cell Monolayer Model

et al. 2020

View full text Add to dashboard Cite

The dysfunction of the intestinal epithelial barrier contributes to local or systemic infection and inflammation. Some lactic acid bacteria (LAB) strains had been shown to improve the conditions of barrier function and, for this reason, are recognized as probiotics. Weissella cibaria, a species belonging to the LAB group, is known to promote several health benefits. However, the role of W. cibaria in regulating the integrity of the intestinal epithelial barrier has not yet been investigated. In this study, W. cibaria MW01 was isolated from Chinese sauerkraut and was selected based on its functional features, such as gastric juice and bile salt tolerance, besides antagonistic activity against pathogenic bacteria. In a cellular model of the intestinal barrier, it was observed that W. cibaria was able to adhere more efficiently than Lactobacillus rhamnosus GG in Caco-2 cells. Moreover, the LPS-induced inflammation in Caco-2 cells was attenuated by the treatment with W. cibaria MW01, which reduced the synthesis of TNF-α, IL-6, and IL-8. In addition, it was noted that the treatment with W. cibaria MW01 recovered the integrity of the Caco-2 cell monolayer exposed to LPS. Furthermore, W. cibaria MW01 significantly alleviated LPS-induced downregulation of tight junction proteins (TJP) (claudin, occludin, and tight junction protein-1). Mechanistically, W. cibaria MW01 inhibited the translocation of NF-κB to the nucleus and deactivated the MLCK-pMLC pathway during LPS exposure. Thus, W. cibaria MW01, as a potential probiotic, can protect intestinal epithelial barrier function by regulating inflammation and expression of TJP via the NF-κB-mediated MLCK-pMLC pathway.

show abstract

Section: Discussionmentioning

confidence: 99%

Weissella cibaria Attenuated LPS-Induced Dysfunction of Intestinal Epithelial Barrier in a Caco-2 Cell Monolayer Model

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The permeability of fully differentiated Caco-2 cells was measured by fluorescein isothiocyanate-dextran-4 diffusion assay. 19 The ultrastructure of fully differentiated Caco-2 cells was observed under a transmission electron microscope (Hitachi, Tokyo, Japan) according to the method of Chen et al 20 The concentrations of TNF-α and IL-8 in fully differentiated Caco-2 cell culture supernatants were determined using ELISA kits, according to the manufacturer's instructions.…”

Section: Intestinal Epithelial Barrier Function Determinationmentioning

confidence: 99%

Postbiotics derived from Lactobacillus plantarum 1.0386 ameliorate lipopolysaccharide-induced tight junction injury via MicroRNA-200c-3p mediated activation of the MLCK-MLC pathway in Caco-2 cells

et al. 2022

View full text Add to dashboard Cite

show abstract

The mechanism of action of Fangji Huangqi Decoction on epithelial-mesenchymal transition in breast cancer using high-throughput next-generation sequencing and network pharmacology

Guo

Pei

Chu

et al. 2022

Journal of Ethnopharmacology

View full text Add to dashboard Cite

Chang’an II Decoction (肠安 II 号方)-Containing Serum Ameliorates Tumor Necrosis Factor-α-Induced Intestinal Epithelial Barrier Dysfunction via MLCK-MLC Signaling Pathway in Rats

Cited by 5 publications

References 38 publications

Weissella cibaria Attenuated LPS-Induced Dysfunction of Intestinal Epithelial Barrier in a Caco-2 Cell Monolayer Model

Weissella cibaria Attenuated LPS-Induced Dysfunction of Intestinal Epithelial Barrier in a Caco-2 Cell Monolayer Model

Postbiotics derived from Lactobacillus plantarum 1.0386 ameliorate lipopolysaccharide-induced tight junction injury via MicroRNA-200c-3p mediated activation of the MLCK-MLC pathway in Caco-2 cells

The mechanism of action of Fangji Huangqi Decoction on epithelial-mesenchymal transition in breast cancer using high-throughput next-generation sequencing and network pharmacology

Contact Info

Product

Resources

About