Intelligent transport systems are used in various transport systems, among which a special place is occupied by crewed autonomous transport systems such as space stations for deep space habitation. These objects have a complex and critical requirement for life support systems (LSSs) to maintain safe and habitable conditions for the crew in the isolated environment. This paper explores the different architectural options for life support systems (LSSs) in autonomous transport systems, specifically focusing on space stations. Three alternative LSS architectures are discussed: Open LSS (OLSS), Closed LSS (CLSS), and Mixed LSS (MLSS). Each architecture has its own advantages and disadvantages. OLSS relies on external resource delivery, reducing initial costs but increasing dependence on resupply missions. CLSS operates autonomously, generating resources onboard, but has higher initial costs and technological complexity. MLSS combines external delivery and onboard generation, providing flexibility and adaptability. The material emphasizes the importance of cost-effectiveness analysis at the early stages of design and identifies the boundary values of mission duration that determine the most effective LSS architecture choice. The material highlights the significance of striking the right balance between cost and performance to develop intelligent ecosystems of LSS for space stations and other autonomous transport systems.