Chandler-Loop surveyed blood compatibility and dynamic blood triggered degradation behavior of Zn-4Cu alloy and Zn

Zhang, Wentai; Li, Ping; Neumann, Beate; Haag, Hanna; Li, Ming; Xu, Zeqian; Zhou, Chao; Scheideler, Lutz; Wendel, Hans-Peter; Zhang, Haijun; Geis‐Gerstorfer, Jürgen; Wan, Guojiang

doi:10.1016/j.msec.2020.111594

Cited by 11 publications

(11 citation statements)

References 61 publications

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“…( O’Connor et al, 2020 ; Wang et al, 2021 ). Thus, Zn-based BMs have attracted ever-increasing attention for over one decade, mainly due to their superior biocompatibility and moderate degradation behavior ( Katarivas et al, 2017 ; Zhang et al, 2021a ; Li et al, 2021 ). Interestingly, Zinc-based nanoscale metal-organic frameworks have attracted a raising attention due to the toxicity of Zn 2+ ( Zhang et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Biodegradable Zn-Cu-Fe Alloy as a Promising Material for Craniomaxillofacial Implants: An in vitro Investigation into Degradation Behavior, Cytotoxicity, and Hemocompatibility

Xu¹,

Xu²,

Zhang³

et al. 2022

Front. Chem.

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Zinc-based nanoparticles, nanoscale metal frameworks and metals have been considered as biocompatible materials for bone tissue engineering. Among them, zinc-based metals are recognized as promising biodegradable materials thanks to their moderate degradation rate ranging between magnesium and iron. Nonetheless, materials’ biodegradability and the related biological response depend on the specific implant site. The present study evaluated the biodegradability, cytocompatibility, and hemocompatibility of a hot-extruded zinc-copper-iron (Zn-Cu-Fe) alloy as a potential biomaterial for craniomaxillofacial implants. Firstly, the effect of fetal bovine serum (FBS) on in vitro degradation behavior was evaluated. Furthermore, an extract test was used to evaluate the cytotoxicity of the alloy. Also, the hemocompatibility evaluation was carried out by a modified Chandler-Loop model. The results showed decreased degradation rates of the Zn-Cu-Fe alloy after incorporating FBS into the medium. Also, the alloy exhibited acceptable toxicity towards RAW264.7, HUVEC, and MC3T3-E1 cells. Regarding hemocompatibility, the alloy did not significantly alter erythrocyte, platelet, and leukocyte counts, while the coagulation and complement systems were activated. This study demonstrated the predictable in vitro degradation behavior, acceptable cytotoxicity, and appropriate hemocompatibility of Zn-Cu-Fe alloy; therefore, it might be a candidate biomaterial for craniomaxillofacial implants.

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Section: Introductionmentioning

confidence: 99%

Biodegradable Zn-Cu-Fe Alloy as a Promising Material for Craniomaxillofacial Implants: An in vitro Investigation into Degradation Behavior, Cytotoxicity, and Hemocompatibility

Xu¹,

Xu²,

Zhang³

et al. 2022

Front. Chem.

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“…This study considered aspects of coagulation and complement activation. Further, the alloy also showed sufficient performance in vivo [ 58 ]. It should be noted, however, that a potential implant is not introduced into a healthy organism.…”

Section: Complement-coagulation Interplaymentioning

confidence: 99%

Predicting the In Vivo Performance of Cardiovascular Biomaterials: Current Approaches In Vitro Evaluation of Blood-Biomaterial Interactions

Strohbach

Busch

2021

IJMS

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The therapeutic efficacy of a cardiovascular device after implantation is highly dependent on the host-initiated complement and coagulation cascade. Both can eventually trigger thrombosis and inflammation. Therefore, understanding these initial responses of the body is of great importance for newly developed biomaterials. Subtle modulation of the associated biological processes could optimize clinical outcomes. However, our failure to produce truly blood compatible materials may reflect our inability to properly understand the mechanisms of thrombosis and inflammation associated with biomaterials. In vitro models mimicking these processes provide valuable insights into the mechanisms of biomaterial-induced complement activation and coagulation. Here, we review (i) the influence of biomaterials on complement and coagulation cascades, (ii) the significance of complement-coagulation interactions for the clinical success of cardiovascular implants, (iii) the modulation of complement activation by surface modifications, and (iv) in vitro testing strategies.

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“…To date, Zn-based BMs have attracted increasing attention and investigation thanks to their superior biocompatibility and moderate degradation behavior [ 8 , 9 , 10 ]. Undoubtedly, ionic Zn, as the main degradation product of Zn-based metals, is an essential mineral in the human body, dominating functional roles in metabolism, immune system and nervous system [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

“…Nonetheless, pure Zn is not without its issues for clinical applications. The main disadvantage of pure Zn is its relatively poor strength and ductility, making it insufficient for load-bearing applications [ 9 , 17 ]. To overcome this shortcoming, mechanical strength can be enhanced by adding alloying elements, mainly including magnesium, silver, copper (Cu) and manganese (Mn), etc.…”

Section: Introductionmentioning

confidence: 99%

“…Regarding in vitro biocompatibility of biodegradable metallic implants, biological safety can mainly be determined by degradation-related factors, mainly including chemical composition, production residues, degradation products, wear debris, and surface effects [ 21 , 22 ]. Thereby, various biological testing methods in the ISO 10993, such as “genotoxicity” (Part 3), “hemocompatibility” (Part 4), “cytotoxicity” (Part 5), and “systemic toxicity” (Part 11), are required to evaluate the novel Zn-based materials [ 9 , 23 ]. The cytotoxicity assessment method is a relatively simple testing method with the advantages of high replicability, accurate results, and standardized assessment.…”

Section: Introductionmentioning

confidence: 99%

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Limitation of Water-Soluble Tetrazolium Salt for the Cytocompatibility Evaluation of Zinc-Based Metals

Zhu

2021

Materials

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Zinc (Zn) and its alloys have been regarded as promising biodegradable metals. The standardized cytotoxicity evaluation is a mandatory step to screen the biocompatibility of novel Zn and its alloys. Nevertheless, the suitability of the tetrazolium-based assay in the direct contact test for some metallic biomaterials (i.e., magnesium and manganese) is questionable. In this study, our results demonstrate an obvious inconsistency between qualitative observation via fluorescence staining and quantitative assessment using water-soluble tetrazolium salt (CCK-8). Subsequent experiments revealed that Zn and pre-treated Zn can directly convert tetrazolium salts to formazan, falsifying the cytotoxicity results. Therefore, we conclude that the CCK-8 assay is not suitable for evaluating the cytotoxicity of biodegradable Zn-based metals in the direct contact test.

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Chandler-Loop surveyed blood compatibility and dynamic blood triggered degradation behavior of Zn-4Cu alloy and Zn

Cited by 11 publications

References 61 publications

Biodegradable Zn-Cu-Fe Alloy as a Promising Material for Craniomaxillofacial Implants: An in vitro Investigation into Degradation Behavior, Cytotoxicity, and Hemocompatibility

Biodegradable Zn-Cu-Fe Alloy as a Promising Material for Craniomaxillofacial Implants: An in vitro Investigation into Degradation Behavior, Cytotoxicity, and Hemocompatibility

Predicting the In Vivo Performance of Cardiovascular Biomaterials: Current Approaches In Vitro Evaluation of Blood-Biomaterial Interactions

Limitation of Water-Soluble Tetrazolium Salt for the Cytocompatibility Evaluation of Zinc-Based Metals

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