Challenging zebrafish escape responses by increasing water viscosity

Danos, Nicole; Lauder, George V.

doi:10.1242/jeb.068957

Cited by 36 publications

(33 citation statements)

References 49 publications

(39 reference statements)

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“…Note that slight changes in water viscosity between the two temperatures (~1.2 and 1.0 mPa s at 11 and 20°C, respectively) were unlikely to have any effects on the fish (Danos and Lauder, 2012).…”

Section: Experimental Protocolmentioning

confidence: 97%

Rainbow trout provide the first experimental evidence for adherence to a distinct Strouhal number during animal oscillatory propulsion

Nudds

John

Keen

et al. 2014

Journal of Experimental Biology

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Section: Experimental Protocolmentioning

confidence: 97%

Rainbow trout provide the first experimental evidence for adherence to a distinct Strouhal number during animal oscillatory propulsion

Nudds

John

Keen

et al. 2014

Journal of Experimental Biology

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“…We separated the relative contribution to size on feeding rates from other ontogenetic effects by conducting a series of dynamic-scaling experiments. In these experiments, we manipulated water viscosities to alter the hydrodynamic regime independently of larval age (35). For the dynamic-scaling experiments, we used three age groups ( Fig.…”

Section: Dynamic-scaling Experimentsmentioning

confidence: 99%

“…Larvae and prey that swim under low Re may swim more slowly than their physically larger counterparts (35). This effect could lead to a reduced encounter rate, which may then result in a reduced feeding rate.…”

Section: Dynamic-scaling Experimentsmentioning

confidence: 99%

Hydrodynamic starvation in first-feeding larval fishes

China

Holzman

2014

Proc. Natl. Acad. Sci. U.S.A.

113

102

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Larval fishes suffer prodigious mortality rates, eliminating 99% of the brood within a few days after first feeding. Hjort (1914) famously attributed this "critical period" of low survival to the larvae's inability to obtain sufficient food [Hjort (1914) Rapp P-v Réun Cons Int Explor Mer 20:1-228]. However, the cause of this poor feeding success remains to be identified. Here, we show that hydrodynamic constraints on the ubiquitous suction mechanism in first-feeding larvae limit their ability to capture prey, thereby reducing their feeding rates. Dynamic-scaling experiments revealed that larval size is the primary determinant of feeding rate, independent of other ontogenetic effects. We conclude that first-feeding larvae experience "hydrodynamic starvation," in which low Reynolds numbers mechanistically limit their feeding performance even under high prey densities. Our results provide a hydrodynamic perspective on feeding of larval fishes that focuses on the physical properties of the larvae and prey, rather than on prey concentration and the rate of encounters.larval ecology | suction feeding | biomechanics | stable ocean hypothesis S tarvation is often considered a major cause of larval fish mortality (1-6). Therefore, the ability of larvae to find and capture food is critical for their survival. Newly hatched fish subsist on a limited supply of yolk and must encounter and successfully capture food before their energy resources become depleted (3, 7). In larval fishes, prey capture success is low at first feeding [occurring 2-4 d post hatching (DPH)] but increases rapidly during early development (3, 8). Correspondingly, larvae undergo a "critical period" of high mortality rates (of up to 99%) after which survival rates increase dramatically (2, 3, 7). The critical period commonly starts at first feeding and lasts 7-10 d, although the duration may vary among species and cohorts. Despite its impact on larval growth and survival, very little is known of the mechanism that underlies this mass mortality, especially during the critical period (1-6).As a larval fish matures, the concomitant increases in its body length and swimming speed alter the outcome of the interaction between the larvae's body and the fluid around it (9, 10). In general, at small body sizes and slow flow speeds organisms experience a hydrodynamic regime of low Reynolds numbers (Re), in which viscous forces (such as drag) dominate. Larger body size and faster flow facilitate a transition into a hydrodynamic regime of higher Re, where inertial forces dominate (see glossary in Table 1 for the Re equation; ref. 10). In larval fishes, this transition was shown to affect fundamental biological processes, such as respiration, metabolism, and swimming (11)(12)(13)(14). Like many adult fishes, larval fish capture their prey using "suction feeding" (Fig. 1): they swim toward it and, when in close proximity, open their mouth while expanding the mouth cavity. The expansion of the mouth generates a strong inward flow of water. This flow exerts a force on ...

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“…To determine whether the RyR channels contributed to normal swimming capabilities, electrically evoked escape responses were recorded and quantified using the DanioVision automated behavioral analysis system (Budick and O'Malley, 2000;Danos and Lauder, 2012) (Fig. 3B).…”

Section: Ryr Channel Function Is Needed For Muscle Contraction Contrimentioning

confidence: 99%

Interactions among ryanodine receptor isotypes contribute to muscle fiber type development and function

Chagovetz

Shaw

Ritchie

et al. 2019

Disease Models &Amp; Mechanisms

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Mutations affecting ryanodine receptor (RyR) calcium release channels commonly underlie congenital myopathies. Although these channels are known principally for their essential roles in muscle contractility, mutations in the human RYR1 gene result in a broad spectrum of phenotypes, including muscle weakness, altered proportions of fiber types, anomalous muscle fibers with cores or centrally placed nuclei, and dysmorphic craniofacial features. Currently, it is unknown which phenotypes directly reflect requirements for RyRs and which result secondarily to aberrant muscle function. To identify biological processes requiring RyR function, skeletal muscle development was analyzed in zebrafish embryos harboring protein-null mutations. RyR channels contribute to both muscle fiber development and function. Loss of some RyRs had modest effects, altering muscle fiber-type specification in the embryo without compromising viability. In addition, each RyR-encoding gene contributed to normal swimming behavior and muscle function. The RyR channels do not function in a simple additive manner. For example, although isoform RyR1a is sufficient for muscle contraction in the absence of RyR1b, RyR1a normally attenuates the activity of the co-expressed RyR1b channel in slow muscle. RyR3 also acts to modify the functions of other RyR channels. Furthermore, diminished RyR-dependent contractility affects both muscle fiber maturation and craniofacial development. These findings help to explain some of the heterogeneity of phenotypes that accompany RyR1 mutations in humans.

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Challenging zebrafish escape responses by increasing water viscosity

Cited by 36 publications

References 49 publications

Rainbow trout provide the first experimental evidence for adherence to a distinct Strouhal number during animal oscillatory propulsion

Rainbow trout provide the first experimental evidence for adherence to a distinct Strouhal number during animal oscillatory propulsion

Hydrodynamic starvation in first-feeding larval fishes

Interactions among ryanodine receptor isotypes contribute to muscle fiber type development and function

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