Challenges of Scale‐up of Cell Separation and Purification Techniques

Hoeve, Marieke A.; Sousa, Paul A. De; Willoughby, Nicholas

doi:10.1002/9781118743362.ch5

Cited by 3 publications

(3 citation statements)

References 79 publications

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“…8,[11][12][13] Furthermore, dielectrophoresis [6][7][8][9]14 and acoustophoresis 10,11 require active force fields, such as electric and acoustic fields, thereby increasing the system's complexity 15 and compromising its scalability. 16 In addition, for sample flow focusing for efficient cell separation, separation methods based on acoustophoresis, 10,11 deterministic lateral displacement, 12 and cell interaction with periodic ridges 13 require buffer input flow in addition to sample flow. In this study, we demonstrate a novel application of inertial microfluidics for the removal of small (<10 μm) nonviable suspended mammalian cells from a cell culture for standard bioreactors.…”

Section: Introductionmentioning

confidence: 99%

Continuous removal of small nonviable suspended mammalian cells and debris from bioreactors using inertial microfluidics

et al. 2018

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Removing nonviable cells from a cell suspension is crucial in biotechnology and biomanufacturing. Labelfree microfluidic cell separation devices based on dielectrophoresis, acoustophoresis, and deterministic lateral displacement are used to remove nonviable cells. However, their volumetric throughputs and test cell concentrations are generally too low to be useful in typical bioreactors in biomanufacturing. In this study, we demonstrate the efficient removal of small (<10 μm) nonviable cells from bioreactors while maintaining viable cells using inertial microfluidic cell sorting devices and characterize their performance. Despite the size overlap between viable and nonviable cell populations, the devices demonstrated 3.5-28.0% dead cell removal efficiency with 88.3-83.6% removal purity as well as 97.8-99.8% live cell retention efficiency at 4 million cells per mL with 80% viability. Cascaded and parallel configurations increased the cell concentration capacity (10 million cells per mL) and volumetric throughput (6-8 mL min −1). The system can be used for the removal of small nonviable cells from a cell suspension during continuous perfusion cell culture operations.

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Section: Introductionmentioning

confidence: 99%

Continuous removal of small nonviable suspended mammalian cells and debris from bioreactors using inertial microfluidics

et al. 2018

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“…1970'lerden beri özellikle beyaz kan ve kök hücrelerin toplanmasında ve plazmaferezde kullanılmaktadır. Konik santrifüj haznesindeki hücre, dışa doğru santrifügal kuvvete ve içe doğru akışkan kuvvete maruz bırakılır (16). Hücreler santrifügal bir kuvvete maruz bırakılırken, seçilen bir ortamda sürekli olarak merkezcil bir yönde akar.…”

Section: Hematopoeti̇k Kök Hücreleri̇n Kli̇ni̇k Uygula-madaki̇ Yeri̇unclassified

“…MACS'ta hücrelerin zenginleştirilmesi, pozitif veya negatif işaretleme yoluyla gerçekleştirilir. Pozitif işaretleme, hedef hücreler doğrudan manyetik boncukla konjuge antikor ile etiketlenir ve daha sonra bu hücreler toplanır; negatifte ise, manyetik boncukla etiketlenmiş hücreler atılır ve etiketlenmeyen hücreler toplanır(16). Şekil 4'de manyetik boncuklarla aktifleştirilmiş, pozitif ve negatif işaretleme ile hücre ayırma yöntemi gösterilmektedir.MACS uygulamasının başarılı örnekleri arasında hastalarda, kemik iliği transplantasyonu yerine insan KK'dan veya MPK'dan izole edilen nadir progenitör hücrelerin kullanılmasıdır(28).…”

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Hematopoeti̇k Kök Hücre İzolasyonunda Güncel Yöntemler

Okşak¹,

Erdem‐Kuruca²

2021

jmed

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Hematopoietic stem cells are the rare cells in various hematopoietic organs which are found in small numbers (1-100/ml). When these cells are transferred to patients suffering from diabetes, cancer, heart diseases, muscle and joint problems, they will help the patients' bodies with the regeneration of impaired tissues. While the most important problem in the transplantation of autologous stem cells is malignant cell contamination in cancer patients, in allogeneic transplantation, it is immune reactions and tissue rejections. Therefore, clearing of the transplant material from tumour cells and immune cells may support the long-term healthy regeneration of the tissue to which they are transplanted. Although various techniques have been developed for the purification of these cells in terms of clinical use over many years, there is still no sufficiently effective method. In recent years, researchers have shown an increased interest in microfluidic systems because they are easy to use, cheap and highly efficient. In these types of systems designed with various microcapillaries, micropillars and micropores; purification is carried out according to the properties of cells such as size, deformability, cell adhesion and electrical charges. This review aims to explain traditional and emerging hematopoietic stem cell isolation methods and their advantages and disadvantages.

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