Challenges in the development of bioanalytical liquid chromatography–mass spectrometry method with emphasis on fast analysis

Nováková, Lucie

doi:10.1016/j.chroma.2012.08.087

Cited by 110 publications

(68 citation statements)

References 123 publications

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“…However, it was pointed out that differences in ionization efficiency can affect the analyte and its IS as it leads to a small difference in retention times, resulting in inconsistent precision and accuracy [21,28]. Therefore, 13 Clabeled compounds are preferred because they are more similar to the corresponding analyte than deuterium-labeled ISs.…”

Section: Effect Of Ismentioning

confidence: 99%

“…Therefore, the reported LC-MS/MS methods suffer from either inadequate sensitivity and/or the need for labor-intensive sample cleanup. This has therefore become a limiting step in the fast analysis of 3-NT [21].…”

Section: Introductionmentioning

confidence: 99%

“…Based on the low endogenous level of free 3-NT in urine and the complexity of the urine matrix, effective sample preparation to specifically remove the matrix and pre-concentrate 3-NT is critical. Solid-phase extraction (SPE) has emerged as a powerful technique for sample purification due to the ample choice of available cartridges with different chemical properties and formats [21]. The traditional reversed-phase cartridge (C18 type) has predominantly been applied for the SPE of 3-NT from biological samples [3,10,16].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Tailored 96-well μElution solid-phase extraction combined with UFLC-MS/MS: a significantly improved approach for determination of free 3-nitrotyrosine in human urine

Li¹,

Shu²,

Kellermann³

2015

Anal Bioanal Chem

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We developed and validated a simple and fast UFLC-MS/MS method for the accurate determination of 3-nitrotyrosine (3-NT) in human urine as a noninvasive biomarker for oxidative stress. The method, involving tailored 96-well μElution solid-phase extraction (SPE) combined with UFLC-MS/MS, allows 3-NT to be determined in biological samples without the need for hydrolysis, derivatization, evaporation, and two-dimensional LC for the first time. Using ammonium acetate (pH 9, 25 mM) as an elution buffer was found to improve SPE selectivity. Fast chromatographic elution of 3-NT with a total run time of 7 min was achieved on a PFPP column (150 mm × 2.1 mm, 3 μm). This fine-tuned integrated method delivered significantly improved throughput, specificity, and sensitivity while reducing the matrix effect, solvent usage, and waste disposal. Using this simple and rapid method, two plates of urine samples (n = 192) can be processed within 24 h. The lower limit of quantification for 3-NT is 10 pg/mL, which represents a notable sensitivity enhancement over reported methods. Less than 6.0 % variations for intraday and interday assay precisions and 97.7-106.3% for accuracies in terms of recovery were obtained. The applicability and reliability of the method were demonstrated by determining the reference range in human urine for 82 healthy people. Considering the noninvasive and inexpensive nature of urine sampling, this novel method could be used to re-evaluate the role of 3-NT as an oxidative stress biomarker in pre-clinical and clinical studies.

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Section: Effect Of Ismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Tailored 96-well μElution solid-phase extraction combined with UFLC-MS/MS: a significantly improved approach for determination of free 3-nitrotyrosine in human urine

Li¹,

Shu²,

Kellermann³

2015

Anal Bioanal Chem

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“…Hydrophilic interaction chromatography (HILIC) has recently become a major separation mode in LC-MS because it can retain and separate hydrophilic compounds that cannot be retained on ODS columns. 4,[9][10][11] In particular, zwitterionic HILIC is a widely accepted separation mode due to its separation capacity. However, it requires millimolar order of bu ering salts that can interfere with analyte ionization.…”

Section: Introductionmentioning

confidence: 99%

“…[1][2][3] Mass spectrometry (MS) is a highly selective and sensitive analytical technique that is important for LC analysis, 4,5) thus the importance of LC-MS instrument is increasing. However, almost all ionization methods (e.g., electrospray ionization (ESI), matrix-assisted laser desorption/ionization (MALDI), and atmospheric pressure chemical ionization (APCI)) used for MS are incompatible with some bu ering salts commonly used for LC (e.g., phosphate, citrate, and Tris), because they o en contaminate the ionization source of mass spectrometer and decrease the ion signal due to bu er-induced ionization suppression.…”

Section: Introductionmentioning

confidence: 99%

Salt Tolerance Enhancement of Liquid Chromatography-Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Using Matrix Additive Methylenediphosphonic Acid

et al. 2014

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Mass spectrometry (MS) is a highly sensitive analytical technique that is o en coupled with liquid chromatography (LC). However, some bu ering salts used in LC (e.g., phosphate and tris(hydroxymethyl) aminomethane (Tris)) are incompatible with MS since they cause ion-source contamination and signal suppression. In this study, we examined salt tolerance of MALDI and applied a matrix additive methylenediphosphonic acid (MDPNA) to reduce salt-induced signal suppression. MDPNA signi cantly improved the salt tolerance of MALDI-MS. Using ammonium formate bu er at pH 5.0, the e ective range of bu ering salt concentration in MALDI-MS using MDPNA was estimated up to 250 mM. MDPNA reduced signal suppression caused by bu ering salts at pH 4.0 to 8.0. We observed that MDPNA e ectively worked over a wide range of bu er conditions. MDPNA was further applied to hydrophilic interaction chromatography (HILIC) and chromatofocusing-MALDI-MS. As a result, the analytes in the eluent containing high-concentration salts were detected with high sensitivity. us, our study provides simple and fast LC-MALDI-MS analysis technique not having strict limitation of bu ering condition in LC by using matrix additive MDPNA.

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Chemometrics in Bioanalytical Chemistry

Yin

Xie

et al. 2016

Encyclopedia of Analytical Chemistry

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Chemometrics, as a young science in chemical discipline, has been widely applied in many fields, such as environmental, clinical and forensic, food, industrial, biological, theoretical, and physical sciences. With the advent of postgenomic era, a powerful tool was urgently needed to extract useful chemical information from huge biological data, which results in the development and application of chemometrics in bioanalytical chemistry. At present, the application of chemometrics in bioanalytical chemistry mainly involves in quantitative bioanalysis and ‘‐omics’. This article focuses on multivariate and multiway calibration in quantitative bioanalysis, which covers fundamentals, current situations, innovative applications, and research trends of these chemometric methods.

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Challenges in the development of bioanalytical liquid chromatography–mass spectrometry method with emphasis on fast analysis

Cited by 110 publications

References 123 publications

Tailored 96-well μElution solid-phase extraction combined with UFLC-MS/MS: a significantly improved approach for determination of free 3-nitrotyrosine in human urine

Tailored 96-well μElution solid-phase extraction combined with UFLC-MS/MS: a significantly improved approach for determination of free 3-nitrotyrosine in human urine

Salt Tolerance Enhancement of Liquid Chromatography-Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Using Matrix Additive Methylenediphosphonic Acid

Chemometrics in Bioanalytical Chemistry

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