Challenges in Developing a Biochip for Intact Histamine Using Commercial Antibodies

Mattsson, Leena; Doppler, Sara; Preininger, Claudia

doi:10.3390/chemosensors5040033

Cited by 7 publications

(5 citation statements)

References 47 publications

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“…More recently a histamine amperometric biosensor that is based on the use of antibody-antigen interaction (immunosensor) was developed to provide higher specificity (Dong et al, 2017). Nevertheless, due to the histamine low molecular weight, simple structure and thus low immunogenicity production of high specificity and affinity antibodies is difficult, significant interference with putrescine were reported and only two of the six commercial antibodies tested in a study by Mattsson et al (2017) bound the histamine free in the solution. Therefore, due to the possible “interferences,” biosensors cannot be used as methods for histamine detection in official controls.…”

Section: Discussionmentioning

confidence: 99%

Biosensing the Histamine Producing Potential of Bacteria in Tuna

et al. 2019

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Histamine poisoning is the most common cause of human foodborne illness due to the consumption of fish products. An enzyme-based amperometric biosensor was developed to be used as a screening tool to detect histamine and histamine-producing bacteria (HPB) in tuna. It was developed by immobilizing histidine decarboxylase and horseradish peroxidase on the surface of screen-printed electrodes through a cross-linking procedure employing glutaraldehyde and bovine serum albumin. The signal generated in presence of histamine at the surface of the electrode was measured by chronoamperometry at in presence of a soluble redox mediator. The sensitivity of the electrode was 1.31–1.59 μA/mM, with a linear range from 2 to 20 μg/ml and detection limit of 0.11 μg/ml. In this study fresh tuna filets purchased in supermarkets in different days ( n = 8) were analyzed to detect HPB. Samples with different concentration of histamine were analyzed with culture-based counting methods, biosensor and HPLC and also a challenge test was made. Recovery of histamine from cultures and tuna samples was also assessed. The presence of Morganella psychrotolerans , Photobacterium phosphoreum , P. damselae and Hafnia alvei was detected using culture- and PCR-based methods. At the time of purchase these tuna samples had histamine concentrations from below the limit of detection (LOD) to 60 μg/g. HPLC and biosensor methods provided similar results in the range from zero to 432 μg/g (correlation coefficient, R 2 = 0.990) and the recovery of histamine from cultures and tuna samples was very high (mean bias −12.69 to 1.63%, with root-mean-square error <12%). These results clearly show that fresh tuna is commonly contaminated with strong HPB. The histamine biosensor can be used by the Food Business Operators as a screening tool to detect their presence and to determine whether their process controls are adequate or not.

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Section: Discussionmentioning

confidence: 99%

Biosensing the Histamine Producing Potential of Bacteria in Tuna

et al. 2019

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“…The assay has a certain complexity and takes several hours, but the measured LoD is remarkably low. Using a conceptually similar approach, Mattson and coworkers developed a histamine assay with colorimetric readout [ 243 , 244 ]: First, histamine was immobilized on sensor chips using a carrier protein. Then, the chips were exposed to samples with spiked histamine concentrations and known amounts of antihistamine antibodies, competing for histamine molecules in solution and immobilized on the chip.…”

Section: Biosensors For Biogenic Amines Using Antibodies and Enzymesmentioning

confidence: 99%

A Review on Bio- and Chemosensors for the Detection of Biogenic Amines in Food Safety Applications: The Status in 2022

Givanoudi

Heyndrickx

Depuydt

et al. 2023

Sensors

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This article provides an overview on the broad topic of biogenic amines (BAs) that are a persistent concern in the context of food quality and safety. They emerge mainly from the decomposition of amino acids in protein-rich food due to enzymes excreted by pathogenic bacteria that infect food under inappropriate storage conditions. While there are food authority regulations on the maximum allowed amounts of, e.g., histamine in fish, sensitive individuals can still suffer from medical conditions triggered by biogenic amines, and mass outbreaks of scombroid poisoning are reported regularly. We review first the classical techniques used for selective BA detection and quantification in analytical laboratories and focus then on sensor-based solutions aiming at on-site BA detection throughout the food chain. There are receptor-free chemosensors for BA detection and a vastly growing range of bio- and biomimetic sensors that employ receptors to enable selective molecular recognition. Regarding the receptors, we address enzymes, antibodies, molecularly imprinted polymers (MIPs), and aptamers as the most recent class of BA receptors. Furthermore, we address the underlying transducer technologies, including optical, electrochemical, mass-sensitive, and thermal-based sensing principles. The review concludes with an assessment on the persistent limitations of BA sensors, a technological forecast, and thoughts on short-term solutions.

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“…Traditional techniques for histamine detection include thin layer chromatography; HPLC and GC; fluorometry; capillary zone electrophoresis; and ELISA (Mattsson et al, 2017). However, these techniques are expensive and time-consuming and require complex sample pretreatment, making them impractical for real-time monitoring in the rumen environment.…”

Section: Sensors For Ruminal Biomarker Concentrationsmentioning

confidence: 99%

Invited review: Sensor technologies for real-time monitoring of the rumen environment

Han

Kaur

Bai

et al. 2022

Journal of Dairy Science

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Quantifying digestive and fermentative processes within the rumen environment has been the subject of decades of research; however, our existing research methodologies preclude time-sensitive and spatially explicit investigation of this system. To better understand the temporal and spatial dynamics of the rumen environment, real-time and in situ monitoring of various chemical and physical parameters in the rumen through implantable microsensor technologies is a practical solution. Moreover, such sensors could contribute to the next generation of precision livestock farming, provided sufficient wireless data networking and computing systems are incorporated. In this review, various microsensor technologies applicable to real-time metabolic monitoring for ruminants are introduced, including the detection of parameters for rumen metabolism, such as pH, temperature, histamine concentrations, and volatile fatty acid concentrations. The working mechanisms and requirements of the sensors are summarized with respect to the selected target parameters. Lastly, future challenges and perspectives of this research field are discussed.

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Challenges in Developing a Biochip for Intact Histamine Using Commercial Antibodies

Cited by 7 publications

References 47 publications

Biosensing the Histamine Producing Potential of Bacteria in Tuna

Biosensing the Histamine Producing Potential of Bacteria in Tuna

A Review on Bio- and Chemosensors for the Detection of Biogenic Amines in Food Safety Applications: The Status in 2022

Invited review: Sensor technologies for real-time monitoring of the rumen environment

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