Challenges in Bone Tissue Regeneration: Stem Cell Therapy, Biofunctionality and Antimicrobial Properties of Novel Materials and Its Evolution

Riester, Oliver; Borgolte, Max; Csuk, René; Deigner, Hans Peter

doi:10.3390/ijms22010192

Cited by 32 publications

(23 citation statements)

References 206 publications

(340 reference statements)

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“…However, it lacks osteogenesis and osteoinductive properties and is prone to collapse, also the absorption and degradation process in the body is very long [50]. Therefore, it is of great significance to develop new bone repair materials with good biocompatibility, suitable speed of biodegradation and antibacterial activity [51][52][53].…”

Section: Discussionmentioning

confidence: 99%

3D printed scaffold for repairing bone defects in apical periodontitis

Liu

Gao

et al. 2022

BMC Oral Health

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Objectives To investigate the feasibility of the 3D printed scaffold for periapical bone defects. Methods In this study, antimicrobial peptide KSL-W-loaded PLGA sustainable-release microspheres (KSL-W@PLGA) were firstly prepared followed by assessing the drug release behavior and bacteriostatic ability against Enterococcus faecalis and Porphyromonas gingivalis. After that, we demonstrated that KSL-W@PLGA/collagen (COL)/silk fibroin (SF)/nano-hydroxyapatite (nHA) (COL/SF/nHA) scaffold via 3D-printing technique exhibited significantly good biocompatibility and osteoconductive property. The scaffold was characterized as to pore size, porosity, water absorption expansion rate and mechanical properties. Moreover, MC3T3-E1 cells were seeded into sterile scaffold materials and investigated by CCK-8, SEM and HE staining. In the animal experiment section, we constructed bone defect models of the mandible and evaluated its effect on bone formation. The Japanese white rabbits were killed at 1 and 2 months after surgery, the cone beam computerized tomography (CBCT) and micro-CT scanning, as well as HE and Masson staining analysis were performed on the samples of the operation area, respectively. Data analysis was done using ANOVA and LSD tests. (α = 0.05). Results We observed that the KSL-W@PLGA sustainable-release microspheres prepared in the experiment were uniform in morphology and could gradually release the antimicrobial peptide (KSL-W), which had a long-term antibacterial effect for at least up to 10 days. HE staining and SEM showed that the scaffold had good biocompatibility, which was conducive to the adhesion and proliferation of MC3T3-E1 cells. The porosity and water absorption of the scaffold were (81.96 ± 1.83)% and (458.29 ± 29.79)%, respectively. Histological and radiographic studies showed that the bone healing efficacy of the scaffold was satisfactory. Conclusions The KSL-W@PLGA/COL/SF/nHA scaffold possessed good biocompatibility and bone repairing ability, and had potential applications in repairing infected bone defects. Clinical significance The 3D printed scaffold not only has an antibacterial effect, but can also promote bone tissue formation, which provides an alternative therapy option in apical periodontitis.

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Section: Discussionmentioning

confidence: 99%

3D printed scaffold for repairing bone defects in apical periodontitis

Liu

Gao

et al. 2022

BMC Oral Health

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“…There is a large focus on emerging preclinical strategies for biomaterials, antimicrobials, and incorporation of new technologies to address fracture-related infections as reviewed in recent literature. [64][65][66][67] Antibiotics for treatment of infections remains the standard of care, but with multiple limitations regarding side effects and limited efficacy. One major limitation to antibiotic usage is inability to prevent formation and penetration of biofilms created by bacteria, thus imparts a large need for problem, including antimicrobial peptides (AMPs), which are small chains of peptides used by the immune system to disrupt cellular membranes or inhibit protein synthesis within bacterial cells (Figure 4).…”

Section: Antimicrobial Agentsmentioning

confidence: 99%

The intersection of fracture healing and infection: Orthopaedics research society workshop 2021

Hellwinkel

Working

Certain

et al. 2022

Journal Orthopaedic Research

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Infection is a common cause of impaired fracture healing. In the clinical setting, definitive fracture treatment and infection are often treated separately and sequentially, by different clinical specialties. The ability to treat infection while promoting fracture healing will greatly reduce the cost, number of procedures, and patient morbidity associated with infected fractures. In order to develop new therapies, scientists and engineers must understand the clinical need, current standards of care, pathologic effects of infection on fractures, available preclinical models, and novel technologies. One of the main causes of poor fracture healing is infection; unfortunately, bone regeneration and infection research are typically approached independently and viewed as two separate disciplines. Here, we aim to bring these two groups together in an educational workshop to promote research into the basic and translational science that will address the clinical challenge of delayed fracture healing due to infection. Statement of clinical significance: Infection and nonunion are each feared outcomes in fracture care, and infection is a significant driver of nonunion. The impact of nonunions on patie[Q2]nt well-being is substantial. Outcome data suggests a long bone nonunion is as impactful on healthrelated quality of life measures as a diagnosis of type 1 diabetes and fracture-related infection has been shown to significantly l[Q3]ower a patient's quality of life for over 4 years. Although they frequently are associated with one another, the treatment approaches for infections and nonunions are not always complimentary and cannot be performed simultaneously without accepting tradeoffs. Furthermore, different

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“…The use of such a model thus reduces the time it takes to translate findings from studies in rodents to humans. There are much interest in the field to develop polymer-or hydrogel-based microfluidic devices for tissue engineering that mimic ion channels in human cells/ tissues, bone tissue regeneration for transplantation, and perhaps tubules found in kidneys, prostate and others (104)(105)(106)(107)(108). A major obstacle of developing an in vitro human testis model is that the polymer-or hydrogel-based microfluidic device, unlike the seminiferous tubules in the testis, is not a dynamic structure.…”

Section: Concluding Remarks and Future Perspectivesmentioning

confidence: 99%

Signaling Proteins That Regulate Spermatogenesis Are the Emerging Target of Toxicant-Induced Male Reproductive Dysfunction

Gao

Wang

et al. 2021

Front. Endocrinol.

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There is emerging evidence that environmental toxicants, in particular endocrine disrupting chemicals (EDCs) such as cadmium and perfluorooctanesulfonate (PFOS), induce Sertoli cell and testis injury, thereby perturbing spermatogenesis in humans, rodents and also widelife. Recent studies have shown that cadmium (e.g., cadmium chloride, CdCl2) and PFOS exert their disruptive effects through putative signaling proteins and signaling cascade similar to other pharmaceuticals, such as the non-hormonal male contraceptive drug adjudin. More important, these signaling proteins were also shown to be involved in modulating testis function based on studies in rodents. Collectively, these findings suggest that toxicants are using similar mechanisms that used to support spermatogenesis under physiological conditions to perturb Sertoli and testis function. These observations are physiologically significant, since a manipulation on the expression of these signaling proteins can possibly be used to manage the toxicant-induced male reproductive dysfunction. In this review, we highlight some of these findings and critically evaluate the possibility of using this approach to manage toxicant-induced defects in spermatrogenesis based on recent studies in animal models.

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Challenges in Bone Tissue Regeneration: Stem Cell Therapy, Biofunctionality and Antimicrobial Properties of Novel Materials and Its Evolution

Cited by 32 publications

References 206 publications

3D printed scaffold for repairing bone defects in apical periodontitis

3D printed scaffold for repairing bone defects in apical periodontitis

The intersection of fracture healing and infection: Orthopaedics research society workshop 2021

Signaling Proteins That Regulate Spermatogenesis Are the Emerging Target of Toxicant-Induced Male Reproductive Dysfunction

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