Challenges in accurate quantitation of lysophosphatidic acids in human biofluids

Onorato, Joelle M.; Shipkova, Petia; Minnich, Anne; Aubry, Anne‐Françoise; Easter, John A.; Tymiak, Adrienne A.

doi:10.1194/jlr.d050070

Cited by 37 publications

(35 citation statements)

References 36 publications

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“…We observed that lysoPtdOH sn ‐1‐18:2 was the most highly abundant molecule from this family (0.56 ± 0.13 μmol/L; Fig. ), which is in agreement with previous literature (Baker et al, ; Onorato et al, ; Shan et al, ). From highest to lowest, the concentrations of the species identified herein were as follows: 18:2 > 22:6 > 20:4 > 16:0 > 18:0 > 18:1.…”

Section: Resultssupporting

confidence: 93%

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Development of a Rapid Ultra High‐Performance Liquid Chromatography/Tandem Mass Spectrometry Method for the Analysis of sn‐1 and sn‐2 Lysophosphatidic Acid Regioisomers in Mouse Plasma

Aristizabal‐Henao

Fernandes

Duncan

2019

Lipids

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Lysophosphatidic acids (lysoPtdOH) are involved in several physiological processes including cell proliferation, inflammation, and glucose metabolism. However, measuring lysoPtdOH is challenging due to inadequate extraction techniques, poor chromatographic resolution, or the inability to discriminate between sn‐1 and sn‐2 regioisomers. In the present work, we developed a high‐throughput (10 min run times) ultra‐high‐performance liquid chromatography–tandem mass spectrometry method capable of discriminating lysoPtdOH species by their fatty acyl composition and sn‐localization on glycerol backbones. We quantitated sn‐1/sn‐2 regioisomeric pairs of lysoPtdOH with 16:0, 18:0, 18:1, 18:2, 20:4, and 22:6 fatty acyl chains using 50 μL of mouse plasma. The method presented here can be expanded to profile more lysoPtdOH species, and has the potential to be used in clinical settings to quickly screen lysoPtdOH profiles. Finally, the ability to discriminate between sn‐1 and sn‐2 isomers can provide insights regarding the metabolic origins and fates of specific lysoPtdOH molecules.

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Section: Resultssupporting

confidence: 93%

“…Spike‐Extract samples, p < 0.05; Fig. ), which is in agreement with previous literature (Jesionowska et al, ; Murph et al, ; Onorato et al, ). Furthermore, losses of approximately 31% were observed in the Spike+Plasma‐Extract samples (0.34 nmol of 0.5 nmol; p < 0.05).…”

Section: Resultssupporting

confidence: 92%

Development of a Rapid Ultra High‐Performance Liquid Chromatography/Tandem Mass Spectrometry Method for the Analysis of sn‐1 and sn‐2 Lysophosphatidic Acid Regioisomers in Mouse Plasma

Aristizabal‐Henao

Fernandes

Duncan

2019

Lipids

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“…Several studies have shown that the concentrations of most molecular lipids are stable after at least one freeze-thaw cycle [9,10,13], but repeated freeze-thawing resulted in a reduced level of most lipid metabolites in both plasma and serum [4]. Some lipids have shown a significant decrease after two freeze-thaw cycles, primarily the PCs [3].…”

Section: Analytical Factors Affecting Lipidomic Analysismentioning

confidence: 95%

Optimizing the lipidomics workflow for clinical studies—practical considerations

Hyötyläinen

Orešič

2015

Anal Bioanal Chem

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Lipidomics is increasingly being used in clinical research, offering new opportunities for disease prediction and detection. One of the key challenges of clinical applications of lipidomics is the high sensitivity of measured lipid levels to many analytical, physiological, and environmental factors, which therefore must be taken into account when designing the studies. Here we critically discuss the complete clinical lipidomics workflow, including selection of the subjects, the sample type, the sample preprocessing conditions, and the analytical method and methods for data processing. We also review the lipidomics applications which investigate the confounding factors such as age, gender, fasting time, and handling procedures for measuring blood lipid metabolites.

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“…Although the basic approach used in our laboratory for these assays has been consistent we have continued to improve these methods to address a number of issues that have arisen in the literature that have been suggested to confound these measurements. This includes suggestions that LPA can be formed by hydrolysis of phospholipids during acidic extractions[25], that LPA can be formed by ion source fragmentation of anionic lysophospholipids during electrospray ionization[26]. The most persistent limitation of these measurements remains sample to sample carry over which can be significant for these lipids and has a major impact on assay precision and robustness.…”

Section: Introductionmentioning

confidence: 99%

Measurement of Lysophosphatidic Acid and Sphingosine-1-Phosphate by Liquid Chromatography-Coupled Electrospray Ionization Tandem Mass Spectrometry

Kræmer

Halder

Smyth

et al. 2017

Methods in Molecular Biology

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Lysophosphatidic acids and sphingosine-1-phosphate are bioactive lipids that regulate diverse cellular and physiological processes through actions that are largely mediated by cell surface receptors. The roles played by these lipids in multiple disease processes make the enzymes and receptors involved in their synthesis, inactivation, and signaling attractive targets for pharmacological therapies. In this chapter we describe methods for sensitive accurate quantitation of LPA and S1P levels in biological fluids using liquid chromatography-coupled electrospray ionization tandem mass spectrometry.

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Challenges in accurate quantitation of lysophosphatidic acids in human biofluids

Cited by 37 publications

References 36 publications

Development of a Rapid Ultra High‐Performance Liquid Chromatography/Tandem Mass Spectrometry Method for the Analysis of sn‐1 and sn‐2 Lysophosphatidic Acid Regioisomers in Mouse Plasma

Development of a Rapid Ultra High‐Performance Liquid Chromatography/Tandem Mass Spectrometry Method for the Analysis of sn‐1 and sn‐2 Lysophosphatidic Acid Regioisomers in Mouse Plasma

Optimizing the lipidomics workflow for clinical studies—practical considerations

Measurement of Lysophosphatidic Acid and Sphingosine-1-Phosphate by Liquid Chromatography-Coupled Electrospray Ionization Tandem Mass Spectrometry

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