Challenges for controlling bovine tuberculosis in South Africa

Arnot, L.F.; Michel, Anita Luise

doi:10.4102/ojvr.v87i1.1690

Cited by 35 publications

(43 citation statements)

References 53 publications

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“…African buffaloes ( Syncerus caffer ) are important wildlife maintenance hosts of M. bovis and serve as a source of infection for other susceptible mammals sharing the same habitat 2 . This highlights the potential disease risk and importance of its control 1 , 2 . Diagnosis of M. bovis infection in African buffaloes typically relies on the measurement of cell-mediated immune (CMI) responses to M. bovis antigens 3 .…”

Section: Introductionmentioning

confidence: 98%

“…Bovine tuberculosis (bTB) is an important zoonosis caused by Mycobacterium bovis ( M. bovis ) and is responsible for severe economic losses and disruption of conservation programs when livestock and wildlife are affected 1 , 2 . African buffaloes ( Syncerus caffer ) are important wildlife maintenance hosts of M. bovis and serve as a source of infection for other susceptible mammals sharing the same habitat 2 .…”

Section: Introductionmentioning

confidence: 99%

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Novel molecular transport medium used in combination with Xpert MTB/RIF ultra provides rapid detection of Mycobacterium bovis in African buffaloes

Clarke

Smith

Goldswain

et al. 2021

Sci Rep

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Mycobacterium bovis is the causative agent of bovine tuberculosis (bTB) in wildlife. Confirmation of M. bovis infection relies on mycobacterial culture, which is time-consuming. Collection and transportation of infectious material also pose a human health risk. PrimeStore Molecular Transport Medium (MTM) has been shown to effectively inactivate infectious organisms, making it a safe method for handling infectious samples. This study investigated an in-field sampling technique for rapid, safe detection of M. bovis in buffalo tissues. Potentially infected tissues from bTB test-positive buffaloes were swabbed at post-mortem examination and stored in PrimeStore MTM at ambient temperature until Xpert MTB/RIF Ultra testing was performed. Additionally, tissue samples were frozen and transported before homogenisation for culture and Ultra testing. Oral swabs were collected from M. bovis-unexposed buffaloes as a negative control cohort. Mycobacterium tuberculosis complex (MTBC) DNA was detected by Ultra in 13/16 tissue swabs and 9/16 matched tissue homogenates from culture-confirmed M. bovis-positive buffalo tissues. MTBC DNA was not detected in swabs from M. bovis-unexposed animals, showing the potentially high specificity of Ultra with PrimeStore swabs. PrimeStore MTM sample processing, in combination with the Ultra assay, has the potential to provide a safe, rapid post-mortem screening test for M. bovis in buffaloes.

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Novel molecular transport medium used in combination with Xpert MTB/RIF ultra provides rapid detection of Mycobacterium bovis in African buffaloes

Clarke

Smith

Goldswain

et al. 2021

Sci Rep

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“…The eradication of M. bovis from domestic herds can be achieved through whole herd depopulation (stamping-out). However, the emergence of financial and animal welfare constraints, as well as the emotional impact made by the farmers and opinion of public community made this strategy be indicated only on rare occasions and under specific circumstances [87].…”

Section: Control and Preventionmentioning

confidence: 99%

Bovine Tuberculosis: A Review of Molecular Diagnostic Methods and Impact on Public Health

Berhanu¹,

Feyisa²,

Mideksa³

et al. 2021

AJMR

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“…The first confirmed case in an African buffalo (Syncerus caffer) in this country occurred within the Hluhluwe-iMfolozi Park in 1986. Afterwards, several cases have been reported in National Parks, game Reserves or private farms, in which buffalos have been implicated in cattle infection [170,171]. Several proposals have been put forward to prevent TB from spreading, including fencing, intensive culling and vaccination.…”

Section: African Buffalomentioning

confidence: 99%

“…Different factors such as age of animals, route of vaccination or challenge dose, among others, were considered. Despite those results, vaccination is considered as a promising strategy and an integral part of TB control in South Africa pending future experiments [ 171 ].…”

Section: Trials In Wildlifementioning

confidence: 99%

Development and Challenges in Animal Tuberculosis Vaccination

et al. 2020

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Vaccination with Bacillus Calmette–Guérin (BCG) constituted a major advance in the prevention of human tuberculosis (TB) in the beginning of the past century. BCG has also a clear potential for use in animals and, in particular, in the main domestic species subjected to TB control programs, cattle. Nowadays, the use of BCG vaccination against TB in cattle is not permitted by European Union legislation because BCG can induce a cellular immune response producing diagnostic interference in the eradication programs based on tuberculin single and comparative intradermal tests imposed worldwide. In this review, we recall the history of TB vaccination as well as different vaccine trials and the response to vaccination in both domestic and wild animals. Promising potential inactivated vaccines are also reviewed. Research studies are mainly focused to improve vaccine efficacy, and at the same time to ensure its easy administration, safety and stability in the environment. Great challenges remain, particularly in terms of vaccine candidates and also in the acceptance of vaccination. Vaccination should be included in a strategic plan for integrated control of TB under a “one health” perspective, which also includes other measures such as improved biosafety on farms to avoid or decrease contact between domestic and wild animals or control of wildlife reservoirs to avoid overabundance that may favor infection maintenance.

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Challenges for controlling bovine tuberculosis in South Africa

Cited by 35 publications

References 53 publications

Novel molecular transport medium used in combination with Xpert MTB/RIF ultra provides rapid detection of Mycobacterium bovis in African buffaloes

Novel molecular transport medium used in combination with Xpert MTB/RIF ultra provides rapid detection of Mycobacterium bovis in African buffaloes

Bovine Tuberculosis: A Review of Molecular Diagnostic Methods and Impact on Public Health

Development and Challenges in Animal Tuberculosis Vaccination

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