2020
DOI: 10.3390/bioengineering7020031
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Challenges and Solutions for Commercial Scale Manufacturing of Allogeneic Pluripotent Stem Cell Products

Abstract: Allogeneic cell therapy products, such as therapeutic cells derived from pluripotent stem cells (PSCs), have amazing potential to treat a wide variety of diseases and vast numbers of patients globally. However, there are various challenges related to the manufacturing of PSCs in large enough quantities to meet commercial needs. This manuscript addresses the challenges for the process development of PSCs production in a bioreactor, and also presents a scalable bioreactor technology that can be a possible soluti… Show more

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Cited by 15 publications
(14 citation statements)
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“…The combination of cell expansion technologies and diverse differentiation protocols has opened the door for creating human-scale, organ-specific cell sources. While these systems have established cell expansion technologies to generate cell quantities sufficient for repopulating humansized organs, the increase in cell numbers begets a more complex microenvironment, which poses a risk of spontaneous differentiation, hindering expansion and directed differentiation capacity [Lee et al, 2020].…”
Section: Bioreactors For Scaling Up Cell Expansion Systemsmentioning
confidence: 99%
“…The combination of cell expansion technologies and diverse differentiation protocols has opened the door for creating human-scale, organ-specific cell sources. While these systems have established cell expansion technologies to generate cell quantities sufficient for repopulating humansized organs, the increase in cell numbers begets a more complex microenvironment, which poses a risk of spontaneous differentiation, hindering expansion and directed differentiation capacity [Lee et al, 2020].…”
Section: Bioreactors For Scaling Up Cell Expansion Systemsmentioning
confidence: 99%
“…Based on the established suspension culture system in 48-well plates, we intended to develop the method to differentiate hESCs into β-like cells in 48-well plates. The diameter of cell aggregates affects mass transfer efficiency and therefore influences differentiation efficiency [26]. Hence, H9 cells at three different densities were initially adopted in this study, including 9 × 10 4 cells/mL, 1.8 × 10 5 cells/mL and 5.4 × 10 5 cells/mL, respectively.…”
Section: Effect Of Cell Density On the Pancreatic Differentiation Efficiency From H9 Cells In 48-well Platesmentioning
confidence: 99%
“…The latter has shown a promising effect when injected in a burn model, in terms of healing and new vessel formation [ 54 ]. However, allogenic cells are less likely to reach the clinic due to several technical challenges, including production in enough numbers, standardization of production and characterization and unexpected immunological events [ 55 ].…”
Section: Reviewmentioning
confidence: 99%